2 amino 5 phosphonopentanoic acid ap5

Drugs were prepared as concentrated stock solutions and diluted in ACSF for bath application immediately prior to use. Gabazine, a selective GABA_A receptor antagonist; (±)-2-amino-5-phosphonopentanoic acid (AP5, a selective NMDA receptor antagonist), 6-cyano-7nitroquinoxaline 2, 3-dione (CNQX, an AMPA-KA receptors antagonist), DL-Serine 2-(2,3,4-trihydroxybenzyl)hydrazide hydrochloride (benserazide), an inhibitor of peripheral dopa decarboxylase and α-methyl-L-tyrosine (levodopa) were all purchased from Sigma Aldrich (France).

Timed-pregnant C57BL/6 mice were purchased from Charles River Laboratories. Biochemicals included 2-amino-5-phosphonopentanoic acid (AP5), D-serine, L-serine, glycine and lithium (Sigma), 7-chlorokynurenic acid (7-CK) (Tocris Bioscience). Antibodies included α-NR1 (BD Pharmagen, mouse monoclonal, Millipore, mouse monoclonal), α-NR2A, α-NR2B (Alomone Labs, rabbit polyclonal), α-PSD-95 (BD Transduction Laboratories, mouse monoclonal; NeuroMab, mouse monoclonal), α-VGLUT1 (Synaptic Systems, polyclonal; mouse monoclonal), α-GAD65 (DSHB, mouse monoclonal), α-gephyrin (Synaptic systems, mouse monoclonal), α-D-serine, α-L-serine, α-Serine Racemase (Abcam, rabbit polyclonal), α-GFAP (Sigma, rabbit polyclonal; NeuroMab, mouse monoclonal), α-GLT1 (obtained from Dr. Michael B. Robinson, a courtesy of Dr. Jeffrey Rothstein, mouse monoclonal; NeuroMab, rabbit polyclonal), α-MAP2 (Abcam, chicken polyclonal), α-GABA (Sigma, rabbit polyclonal), α-VAMP2 (Synaptic systems, mouse monoclonal).

Antibodies and chemicals used in this report are as follows: rabbit polyclonal syndecan-2 antibody (syndecan-2G, 1:1000)18 (link); mouse monoclonal PSD-95 (K28/43, MABN68, 1:1000, Millipore)43 (link); mouse monoclonal synaptophysin (S5768, 1:1000, Sigma)44 (link); mouse monoclonal Tubulin (B-5-1-2, 1:5000, Sigma)43 (link); chicken polyclonal GFP (ab13970, 1:5000, Abcam)43 (link); mouse monoclonal GFP (JL-8, 632381, 1:2000, Clontech); rabbit polyclonal Cherry (632496, 1:1000, Clontech); rat monoclonal CD8α (14-0081, 1:1000, eBioscience)45 (link); mouse monoclonal VCP (612183, 1:1000, BD Biosciences)46 (link); mouse monoclonal Myc (9B11, #2276, 1:1000, Cell Signaling Technology)43 (link); mouse monoclonal CASK (K56A, MAB5230, 1:500, Millipore)22 (link); mouse monoclonal Oyster-550-conjugated synaptotagmin (105 311C3, 1:400, Synaptic System); Alexa Fluor-488- and-555-conjugated secondary antibodies (Invitrogen); Sodium chlorate (Sigma); Ethylene glycol tetraacetic acid (EGTA, Sigma); 2-amino-5-phosphonopentanoic acid (AP5; Sigma); KN-92 (Cayman chemical); KN-93 (Sigma); CK-59 (Calbiochem).