Lc ms 8040

All electronic abosorption spectra were obtained on either a Varian Cary 50 Bio spectrophotometer in a 2 ml quartz cuvette or a NanoDrop One^C between 200 nm and 800 nm. Fluorescence spectra was obtained on an Agilent (Santa Clara, CA, USA) Cary Eclipse Fluorescence Spectrophotometer in a 2‐ml cuvette between 565 nm and 800 nm. Mass spectrometry data were acquired on a Shimadzu (Columbia, MD, USA) LC‐MS 8040 and/or a Bruker Autoflex III Smartbeam matrix‐assisted laser desorption/ionization time of flight (MALDI‐TOF) using alpha‐cyano‐4‐hydroxycinnamic acid or dihydroxybenzoic acid as matrix. Circular dichroism was performed in a 200‐μl quartz cuvette on an Applied Photophysics Chirascan VX instrument. Cell assays were prepared and ran using a Memmert iso150 Incubator and Molecular Devices FlexStation 3. Cell assays were run using a Ready‐to‐Assay OT receptor cell kit (Millipore Sigma HTS090RTA) for Calcium Flux FLIPR Assays. Peptides were prepared on a CEM Liberty Blue automated microwave peptide synthesizer (CEM Corporation) and cleaved from resin using a CEM Razor rapid peptide cleavage system in 95% TFA containing 2.5% triisopropylsilane and 2.5% water for 40 min at 40°C. Peptides were purified using an Agilent 1200 reverse‐phase high‐performance liquid chromatography (RP‐HPLC). Products were dried on a 1 L Labconco FreeZone lyophilizer.