Ly6g clone 1a8

Mice, 129S2 (129SvPas), Ifnar1^−/− in 129S2 background 24 (link), and Ifnar1^−/−44 (link) in C57BL/6 background were bred and kept under specific pathogen-free conditions at the Max Planck Institute for Infection Biology in Berlin, Germany. C57BL/6 animals were purchased from Charles Rivers Laboratories. Mice were 10–12 weeks of age at the beginning of the experiments. Aerosol infection with Mtb strain H37Rv and enumeration of bacteria in lung tissue were performed as previously described 34 (link). To generate GFP-expressing Mtb, competent H37Rv was transformed with the plasmid pGFM-11 by electroporation and selected on kanamycin 45 (link). Chimeric mice were obtained by reconstituting lethally irradiated mice with BM cells. Mtb infection was performed 8–10 weeks following transplantation. PMNs were depleted by administration of 250 μg mAb Ly6G (clone 1A8, BioXCell) i.p. for 1 week at 2-day intervals, starting 7 days p.i. All experiments were performed in accordance with German Animal Protection Law, in Berlin, by the State Office of Health for Social Affairs in Berlin (Landesamt für Gesundheit and Soziales, LAGeSo; approvals G0310/06, G0221/11, T0223/11, and T0087/13).