Proteonmanager version 3

Manufactured by Bio-Rad

ProteOnManager Version 3.1.0.6 is a software application developed by Bio-Rad for data acquisition and analysis of protein interaction experiments using the ProteOn XPR36 Protein Interaction Array System. The software provides a user interface for system control, data collection, and data processing.

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Lab products found in correlation

Proteon xpr36 protein interaction array system, by Bio-Rad (2 mentions) Htg sensor chip, by Bio-Rad (1 mentions)

2 protocols using proteonmanager version 3

Quantifying PCNA-Antibody Interactions

Cited 1 time

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A ProteOn™ XPR36 Protein Interaction Array System (Bio-Rad) was
used to measure the affinity of the monoclonal antibody 14-25-9 to His-tagged
recombinant PCNA. For the assay, a HTG sensor chip (#1765031) and ProteOn
Manager Version 3.1.0.6 (Bio-Rad Laboratories) were employed. After activation
of the chip using EDC/S-NHS amine coupling procedure, the ligand immobilization
process was performed with His-tagged recombinant PCNA and His-tagged IL2 as
control, at a flow rate of 30μl/min in different flow cells. Different
analyte (14-25-9) concentrations (0–100nM) were injected at a flow rate
of 40μl/min, with regeneration of the surface using 50mM NaOH after each
analyte. Data was analyzed using the bivalent binding model (40 (link)).

Kundu K., Ghosh S., Sarkar R., Edri A., Brusilovsky M., Gershoni-Yahalom O., Yossef R., Shemesh A., Soria J.C., Lazar V., Ben-Zion J., Campbell K.S., Elkabets M, & Porgador A. (2019). Inhibition of the NKp44-PCNA Immune Checkpoint Using a mAb to PCNA. Cancer immunology research, 7(7), 1120-1134.

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Quantifying NKp44 Peptide-PCNA Interactions

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A ProteOn™ XPR36 Protein Interaction Array System (Bio-Rad) was used to measure the affinity of NKp44-derived peptides to MBP-fused recombinant hPCNA. For the assay, a GLC sensor chip (7CG21401) and ProteOn Manager Version 3.1.0.6 (Bio-Rad Laboratories) was employed. After activation of the chip using EDC/S-NHS amine coupling procedure, the ligand immobilization process was performed with NKp44-pep8, R11-NLS-pep8, R11-NLS-pep8short, and BSA as a control at a flow rate of 30 µl/min in different flow cells. Different concentrations (0–250 nM) of analyte (MBP-PCNA) were then injected at a flow rate of 25 µl/min. Regeneration of the surface was done using 50 mM NaOH. Data were analyzed using equilibrium analysis model.

Shemesh A., Kundu K., Peleg R., Yossef R., Kaplanov I., Ghosh S., Khrapunsky Y., Gershoni-Yahalom O., Rabinski T., Cerwenka A., Atlas R, & Porgador A. (2018). NKp44-Derived Peptide Binds Proliferating Cell Nuclear Antigen and Mediates Tumor Cell Death. Frontiers in Immunology, 9, 1114.

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