Log In Sign up
The largest database of trusted experimental protocols

A1 scanning confocal microscope

Manufactured by Nikon
Visit Supplier
Sourced in Japan

The Nikon A1 is a scanning confocal microscope designed for high-resolution imaging. It utilizes laser scanning technology to capture detailed, three-dimensional images of samples with improved optical resolution and contrast compared to conventional microscopes.

Automatically generated - may contain errors

Lab products found in correlation

Dic plan apochromat vc objective, by Nikon (4 mentions) Alexa fluor 594 goat anti mouse igg, by Thermo Fisher Scientific (2 mentions) Eclipse tie microscope stand, by Nikon (2 mentions) Nis element, by Nikon (2 mentions) Metamorph 7, by Molecular Devices (1 mentions) Tcs sp8 confocal microscope, by Leica (1 mentions) Plan apo oil objective, by Nikon (1 mentions) Lysotracker red dnd 99, by Thermo Fisher Scientific (1 mentions) Mouse anti nkx6, by Developmental Studies Hybridoma Bank (1 mentions) Rabbit anti somatostatin, by Agilent Technologies (1 mentions) Rabbit anti glucagon, by Agilent Technologies (1 mentions) Guinea pig anti insulin, by Agilent Technologies (1 mentions) Rabbit anti dsred, by Takara Bio (1 mentions) Insulin, by Thermo Fisher Scientific (1 mentions) Eclipse ti inverted microscope, by Nikon (1 mentions) α tubulin, by Merck Group (1 mentions) Anti cd133, by Cell Signaling Technology (1 mentions) Mitotracker green fm, by Thermo Fisher Scientific (1 mentions) Phospho histone h2a x ser139, by Merck Group (1 mentions) Dm5500b, by Leica (1 mentions)

Close spelling variants of this product

A1+ inverted confocal laser scanning microscope Nikon A1 confocal laser scanning microscope system Nikon A1+ point scanning confocal microscope A1-RS scanning confocal microscope Nikon A1R/A1 laser scanning confocal microscope A1 resonant scanning confocal microscope (A1R-SIMe). A1 resonant scanning confocal microscope TiE A1-R laser scanning confocal microscope Nikon A1 + confocal laser scanning microscope (CLSM) Ti-E scanning laser confocal inverted microscope (A1)

30 protocols using a1 scanning confocal microscope

1

Quantifying Neuronal Dendritic Morphology

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary hippocampal neurons were fixed with 4% paraformaldehyde plus 4% sucrose for 20 min and incubated with primary antibodies overnight at 4°C, followed by secondary antibodies at room temperature for 1 h. Images were taken under a Nikon A1 scanning confocal microscope with a 40× or 60× oil immersion objective. Mouse brain sections were stained with anti-GFP antibody in Tris-buffered saline containing 0.1% Triton X-100 and 3% bovine serum albumin. Images of the hippocampal region were captured using a Leica TCS SP8 confocal microscope with a 40× oil immersion objective. The number, length, and complexity of neuronal dendrites were quantified using ImageJ (National Institute of Health, Bethesda, MA, USA) with the NeuronJ [17 (link)] and Sholl analysis plugins (Anirvan Ghosh). Dendritic spines were quantified using Metamorph 7.0 (Molecular Devices). All data are presented as mean ± SEM. Statistical comparisons were performed using Student’s t-test and one-way ANOVA.
Chen Y., Liang Z., Fei E., Chen Y., Zhou X., Fang W., Fu W.Y., Fu A.K, & Ip N.Y. (2015). Axin Regulates Dendritic Spine Morphogenesis through Cdc42-Dependent Signaling. PLoS ONE, 10(7), e0133115.
+ Open protocol
+ Expand
2

Confocal Microscopy for High-Resolution Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
Confocal microscopy images were obtained on a Nikon A1 scanning confocal microscope with 100x magnification. The excitation wavelength was 561 nm and detection was set for Cy3 fluorescence using a GaAsP detector. The diffraction-limited spatial resolution is 260 nm. Images were processed with ImageJ and modified only for contrast and brightness. Images were cropped and enlarged to aid observation of the smallest features.
Dutagaci B., Nawrocki G., Goodluck J., Ashkarran A.A., Hoogstraten C.G., Lapidus L.J, & Feig M. (2021). Charge-driven condensation of RNA and proteins suggests broad role of phase separation in cytoplasmic environments. eLife, 10, e64004.
+ Open protocol
+ Expand
3

Multicolor Confocal Imaging Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Imaging was performed on a Nikon A1 scanning confocal microscope, using either a 60x or 40x/1.3 NA Plan-Apo oil objective. Z stacks (500 – 1000 nm steps) were acquired in using the 405nm diode laser, 561nm diode-pumped-solid-state (DPSS) laser, a 638nm diode laser, as well as a 488nm Argon-gas laser line.
Alsous J.I., Villoutreix P., Berezhkovskii A.M, & Shvartsman S.Y. (2017). Collective Growth in a Small Cell Network. Current biology : CB, 27(17), 2670-2676.e4.
+ Open protocol
+ Expand
4

Lysosomal Activity Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
The lysosomal activity of the cells was measured using LysoTracker® Red DND‐99 (Life Technologies, USA), which can accumulate in acidic compartments such as lysosomes due to proton trapping. For this experiment, LysoTracker media was made with a final concentration of 50 nM in normal cell culture media prior to staining and kept at 37°C in the dark until use. After removal of the original media at the end point of designated treatment, LysoTracker media was added, the cells were incubated for 20 min. Once the Lysotracker media was removed, cells were washed twice with PBS and fixed with 3% glutaraldehyde (Aladdin Biochemical Technology, China) in H2O followed by nuclear staining with DAPI. Images were obtained with a Nikon A1 scanning confocal microscope. The number of Lysotracker+ dots was determined by randomly selecting 3 areas for each sample using Image J software.
Wang X., Wu R., Zhai P., Liu Z., Xia R., Zhang Z., Qin X., Li C., Chen W., Li J, & Zhang J. (2023). Hypoxia promotes EV secretion by impairing lysosomal homeostasis in HNSCC through negative regulation of ATP6V1A by HIF‐1α. Journal of Extracellular Vesicles, 12(2), e12310.
+ Open protocol
+ Expand
5

Immunofluorescent Staining of Larval Gut Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
For whole mount immunofluorescent staining, larvae were fixed in 4% paraformaldehyde at 4°C overnight. Following fixation, the whole gut region was dissected out and blocked for 1 hour with PBS with 0.2% Triton (PBST) and 10% fetal bovine serum. For cryosections, fish were similarly fixed and the gut-intestine system was dissected. Tissues were then immersed in 30% sucrose/PBS, embedded in optimal cutting temperature (OCT) compound, frozen in liquid nitrogen, and sectioned in 10 µm thickness using a cryostat. Primary antibodies used in this study included: mouse anti-Nkx6.1 (Developmental Studies Hybridoma Bank, F55A12), 1: 100. Guinea pig anti-Insulin (Dako, A0564), 1: 500. Rabbit anti-Glucagon (Dako, A0565), 1:400. Rabbit anti-Somatostatin (Dako, A0566), 1:500. Rabbit anti-DsRed (Clontech, 642496), 1:100. Mouse anti-2F11 (Abcam, ab71286), 1:200. Primary antibodies were incubated at 4°C overnight. After washing with PBST, samples were incubated with secondary antibodies (Jackson Immunoresearch, 1:300) in blocking buffer. Fluorescent images were acquired with Nikon A1 scanning confocal microscope. Cell counting was carried out manually. Briefly, whole mount tissues were scanned by confocal microscope and maximum projections were assembled from Z-stacks. Cell numbers were counted from reconstructed images. Students t-test was implemented for statistical analysis.
Wang Y.J., Park J.T., Parsons M.J, & Leach S.D. (2015). Fate mapping of ptf1a-expressing cells during pancreatic organogenesis and regeneration in zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists, 244(6), 724-735.
+ Open protocol
+ Expand
6

Imaging Migrating Border Cells in Drosophila Ovaries

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Male flies containing slbo-GAL4, UAS-lifeact-GFP and UAS-LacZ were crossed to UAS-raskol-RNAi females. 1–2 day old F1 females were incubated at 29°C for 1–2 days in vials with fresh yeast paste and slbo-GAL4, UAS-lifeact-GFP males. Ovaries were dissected for live imaging in imaging media (Schneiders’s medium, 15% fetal bovine serum (FBS) and 0.2mg/ml Insulin; Thermo Fisher Scientific) according to published protocols [22 (link), 98 (link)]. 100 μl of imaging media containing egg chambers was transferred to poly-D-lysine coated Mattek dishes for imaging. 20 μm z-stacks (1 μm step size) covering the whole migrating border cell cluster were acquired every 2 minutes using on a Nikon A1 scanning confocal microscope.
Raza Q., Choi J.Y., Li Y., O’Dowd R.M., Watkins S.C., Chikina M., Hong Y., Clark N.L, & Kwiatkowski A.V. (2019). Evolutionary rate covariation analysis of E-cadherin identifies Raskol as a regulator of cell adhesion and actin dynamics in Drosophila. PLoS Genetics, 15(2), e1007720.
+ Open protocol
+ Expand
7

RNAi Analysis of Border Cell Migration

Check if the same lab product or an alternative is used in the 5 most similar protocols
For BC migration analysis, RNAi-expressing female flies under the control of slbo-GAL4 were collected and transferred to vials containing fresh yeast paste and males. Flies were raised at 29°C for 1–2 days. UAS-GFP was used as a reporter for RNAi expression. Dissected ovaries were fixed in 4% paraformaldehyde in PBS for 20 mins and washed 5 times with PBS. Ovaries were mounted on microscope slides in 70% glycerol and 20 μm z-stacks were acquired on a Nikon A1 scanning confocal microscope.
Raza Q., Choi J.Y., Li Y., O’Dowd R.M., Watkins S.C., Chikina M., Hong Y., Clark N.L, & Kwiatkowski A.V. (2019). Evolutionary rate covariation analysis of E-cadherin identifies Raskol as a regulator of cell adhesion and actin dynamics in Drosophila. PLoS Genetics, 15(2), e1007720.
+ Open protocol
+ Expand
8

Glyoxal-Induced Stress on Endothelial Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were plated on 0.17 mm glass coverslips (no. 1.5) pre-coated with gelatin and allowed to adhere to a monolayer. BAOECs were pre-treated with RAP [5µg/ml] for 2h and then glyoxal 500µM was added to the medium for 24h. Cells were washed in phosphate-buffered saline (PBS), fixed in 4% para-formaldehyde (PFA), permeabilized in 0.1% Triton PBS 1X for 2 min on ice, incubated with different primary Abs and revealed by appropriate Alexa-Fluor-tagged secondary Abs (Thermo Fisher Scientific). Cells were analyzed by using a Nikon Ti Eclipse inverted microscope with A1 scanning confocal microscope at the Confocal and Specialized Microscopy Shared Resource (CSMSR). 60x/1.49 Apo TIRF Oil immersion objective was employed. 1024x1024 pixel images were acquired using NIS Elements software. The acquisition was performed by adopting a laser power, gain, and offset settings that allowed maintaining pixel intensities (gray scale) within the 0-255 range and hence avoid saturation. Analysis of confocal images was performed using the ImageJ software
Camillo C., Abramov A., Allen P.J., Castillero E., Roberts E., Xue Y., Frasca A., Moreno V., Kurade M., Robinson K., Spiegel D.A., LaPar D.J., Grau J.B., Assoian R.K., Bavaria J.E., Takayama H., & Ferrari G. (2021). RAGE antagonist peptide mitigates AGE-mediated endothelial hyperpermeability and accumulation of glycoxidation products in human ascending aortas and in a murine model of aortic aneurysm.
+ Open protocol
+ Expand
9

Multimarker Imaging of Glioblastoma Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
To label active mitochondria, live glioblastoma cells were incubated with MitoTracker Green FM (Molecular Probes #M7514) using on Invitrogen Molecular Probes protocol. Cells were fixed with 4% paraformaldehyde in PBS for 30 min. Then immunochemical staining was performed using standard protocols. Glioblastoma cells were stained with α-Tubulin (mAb #T5168 from Sigma) and phospho-Histone H2A.X Ser139 (mAb #05-636 from Millipore), as well with anti-CD133 (mAb #64326 from Cell Signaling Technology). The secondary Abs were Alexa Fluor 594 goat anti-mouse IgG from Molecular Probes/Life Technologies (Carlsbad, CA, USA). A Nikon A1 scanning confocal microscope on an Eclipse TiE microscope stand (Nikon Instruments, Melville, NY) was used for immunofluorescence image acquisition (as Z-series projection) and analysis. Images were further analyzed and quantified using ImageJ software (NIH).
Ivanov V.N., Wu J., Wang T.J, & Hei T.K. (2019). Inhibition of ATM kinase upregulates levels of cell death induced by cannabidiol and γ-irradiation in human glioblastoma cells. Oncotarget, 10(8), 825-846.
+ Open protocol
+ Expand
10

Live Imaging of Drosophila Embryos

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Raskol-YFP [55 (link)] homozygous female flies were crossed to DE-cad-mCherry [49 (link)] homozygous males. Embryos were collected overnight on grape juice agar plates and transferred to microscope slides coated with double-sided tape. Embryos were manually dechorionated and immediately transferred to halocarbon oil on coverslips with the dorsal side facing down. Coverslips were then attached to imaging chambers using double sided tape and imaged on a Nikon A1 scanning confocal microscope.
Raza Q., Choi J.Y., Li Y., O’Dowd R.M., Watkins S.C., Chikina M., Hong Y., Clark N.L, & Kwiatkowski A.V. (2019). Evolutionary rate covariation analysis of E-cadherin identifies Raskol as a regulator of cell adhesion and actin dynamics in Drosophila. PLoS Genetics, 15(2), e1007720.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!