The human monoclonal antibodies (mAbs) used for flow cytometry in this article included FITC-anti-CD3, FITC-anti-CD80, FITC-anti-CD163, FITC-anti-CD8α, FITC-anti-CD14, PE-anti-CD40, APC-anti-IFN-γ (all from BioLegend), BB515-anti-TLR2, and PE-anti-CD206 (all from BD Biosciences). Cells were scrapped from culture plates and incubated with human AB serum to saturate non-specific mAb binding before staining with the indicated fluorophore-labeled mAbs. For intracellular IFN-γ staining, cells were stimulated with Cell Activation Cocktail (with Brefeldin A; BioLegend) for 6 h according to the manufacturer's protocol. All data were collected using Novocyte flow cytometer (ACEA) and analyzed with FlowJo software.
Fitc anti cd8α
Manufactured by BioLegend
Sourced in United States
FITC-anti-CD8α is a fluorochrome-conjugated antibody that targets the CD8α protein, which is expressed on the surface of cytotoxic T cells. This product can be used for the identification and enumeration of CD8+ T cells in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti ifn γ apc, by BioLegend (2 mentions)
Anti cd3 fitc, by BioLegend (1 mentions)
Anti cd40 pe, by BioLegend (1 mentions)
Anti cd14 fitc, by BioLegend (1 mentions)
Fitc anti cd80, by BioLegend (1 mentions)
Anti cd206 pe, by BD (1 mentions)
Novocyte flow cytometer, by Agilent Technologies (1 mentions)
Cell activation cocktail with brefeldin a, by BioLegend (1 mentions)
Accuri c6, by BD (1 mentions)
Anti cd4 fitc, by BioLegend (1 mentions)
Anti cd11b pe, by BioLegend (1 mentions)
Brefeldin a, by BioLegend (1 mentions)
Apc anti gr1, by BioLegend (1 mentions)
Pe anti il 4, by BioLegend (1 mentions)
2 protocols using fitc anti cd8α
1
Flow Cytometry Immunophenotyping Protocol
2
Flow Cytometric Analysis of Cytokine Production
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Briefly, 1×106 splenocytes for each mouse were plated into 96-well plates and stimulated with 5 μg/mL E744-62 for 6 h, and 5 μg/mL Brefeldin A (Biolegend, San Diego, CA, USA) was added to allow the accumulation of intracellular cytokines. The cells were first stained with FITC-anti-CD4 (Biolegend, USA), FITC-anti-CD8α (Biolegend, USA), or PE-anti-CD11b (Biolegend, USA) and APC-anti-Gr1 (Biolegend, USA). Subsequently, fixation/permeabilization and intracellular staining for APC-anti-IFNγ (Biolegend, USA) or PE-anti-IL4 (Biolegend, USA) was performed. The samples were analysed by BD Accuri C6 (BD Biosciences, Germany), and the data were analysed using Flowjo software 7.6.
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