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Potassium ferrocyanide 3

Manufactured by Merck Group
Sourced in Australia

Potassium ferrocyanide(III) is a chemical compound with the formula K4[Fe(CN)6]. It is a crystalline, yellow-colored salt that is soluble in water. Potassium ferrocyanide(III) serves as a common source of the ferrocyanide ion, which is used in various chemical applications.

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5 protocols using potassium ferrocyanide 3

1

Electrochemical Characterization of Organic Compounds

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All reagents and solvents were
of commercial reagent grade and were used without further purification,
except where noted. Reagents not listed were purchased from Sigma-Aldrich.
4-(2-Aminomethyl) pyridine (98%), 4-methylpyridine (99%), 4-propylpyridine,
4-aminopyridine (98%), potassium ferrocyanide(III) (99%), sodium nitrite
(97%), silver nitrite (99%), deuterium oxide (D2O), (>99.8%
D), and potassium bicarbonate (99.7%) were purchased from Sigma-Aldrich
Company. All aqueous solutions were prepared using Millipore water
(18.2 MΩ cm). The glassy carbon surface was polished with 1,
0.3, and 0.05 μm alumina slurries. The electrodes were then
ultrasonicated in acetone, ethanol, and water.
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2

Characterization of Botanical Extracts

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Sigma-Aldrich Aldrich (Castle Hill, NSW, Australia) provided most of the chemicals utilized for extraction and characterization. Gallic acid, Folin–Ciocalteu’s phenol reagent, L-ascorbic acid, sodium phosphate, vanillin, aluminum chloride hexahydrate, iron(III) chloride hexahydrate (FeCl3·6H2O), sodium phosphate monobasic monohydrate, sodium phosphate dibasic heptahydrate, trichloroacetic acid, hydrochloric acid, ethylenediaminetetraacetic acid (EDTA), ferrozine, ammonium molybdate, 3-hydroxybenzoic acid, catechin, iron (II) sulfate heptahydrate, potassium ferrocyanide(III), DPPH, 2,4,6 tripyridyl-s-triazine (TPTZ), and ABTS were purchased from Sigma Aldrich (Castle Hill, NSW, Australia). From Chem-Supply Pty Ltd. (Adelaide, SA, Australia), we purchased sodium carbonate anhydrous and hydrogen peroxide (30%), and we also purchased 98% H2SO4 from RCI Labscan (Rongmuang, Thailand). Thermo Fisher Scientific Inc. supplied methanol, ethanol, acetonitrile, formic acid, glacial acetic acid, iron (III) chloride anhydrous, and HPLC and LC-MS-grade chemicals (Scoresby, VIC, Australia). Thermo Fisher Scientific provided 96-well plates for various in vitro bioactivities and antioxidant tests (Scoresby, VIC, Australia). HPLC vials (1 mL) were also purchased from Agilent equipment (Melbourne, VIC, Australia).
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3

Analytical Characterization of Polyphenols

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Analytical-grade chemicals were the only ones utilized in all the tests. Chemicals for identifying and characterizing compounds were supplied by Sigma Aldrich, located in Darmstadt, Germany. To estimate polyphenols and antioxidant capacity, the following materials were purchased from Sigma Aldrich (Castle Hill, NSW, Australia): potassium ferrocyanide (III), hydrated sodium acetate, trichloroacetic acid, vanillin, ammonium molybdate, sodium phosphate dibasic heptahydrate, Folin–Ciocalteu’s reagent, 2,2′-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS), ethylenediaminetetraacetic acid (EDTA), catechin, 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), 2,4,6 tripyridyl-s-triazine (TPTZ), and 3-hydrobenzoic acid, as well as quercetin.
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4

Fabrication and Characterization of AuIDEs

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Gold (Au) deposited onto glass substrate electrodes (AuEs) were lithographically fabricated at the National Synchroton Laboratory facilities (Campinas, Brazil) and generously facilitated by the GNano research group of the São Carlos Institute of Physics (IFSC)-USP (Brazil). Thin-film Gold InterDigitated Electrode (AuIDEs) with 180 pairs of interdigitated gold electrodes (5/5 µm, electrode/gap) were obtained from Micrux Technologies, Spain.
Etilic alcohol, acetone, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), N-hydroxysuccinimide (NHS), phosphate-buffered saline (PBS), 30% Hydrogen Peroxide, 30% Ammonium Hydroxide, and potassium ferrocyanide (III) powder <10 um, 99% (702587) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Potassium hydroxide (KOH) and cysteamine >98.0% (30070) were purchased from Sigma-Aldrich (São Paulo, Brazil). Potassium chloride (P217500) ACS 99.0 a 100% was obtained from Fisher (Hampton, NH, USA). All reagents used were of analytical grade. Deionized water (MiliQ®, Merck-Millipore, Molsheim, France) was employed to prepare all solutions. Recombinant Anti-S100 beta antibody [EP1576Y]-Astrocyte Marker, Recombinant Human S100 beta protein (ab55570), and Recombinant Human nNOS (neuronal) protein (ab159005) were acquired from Abcam (Cambridge, MA, USA).
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5

Polyphenols and Antioxidant Evaluation

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In all experiments, only analytical-grade chemicals were used. Sigma Aldrich (Darmstadt, Germany) provided the chemicals for the characterization and identification of compounds. Folin–Ciocalteu’s reagent, hydrated sodium acetate, vanillin, sodium phosphate dibasic heptahydrate, trichloroacetic acid, ethylenediaminetetraacetic acid (EDTA), ammonium molybdate, catechin, 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), potassium ferrocyanide (III), 2,4,6 tripyridyl-s-triazine (TPTZ), 2,2’-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 3-hydrobenzoic acid, and quercetin were purchased from the Sigma Aldrich (Castle Hill, NSW, Australia) for the estimation of polyphenols and antioxidant capability.
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