Reflectance spectroscopy measurements were performed under sample direct illumination in a dual-beam spectrophotometer (Agilent Technologies Cary 5000 UV-Vis-NIR) equipped with a diffuse reflectance accessory. Fluorescence spectra for samples in air and in aqueous 3-(N-morpholino)propane sulfonic acid (MOPS) buffer in a quartz cuvette were measured under 355 nm irradiation of the samples by a passively Q-switched powerchip laser (Teem Photonics PNV-M02510) operating in pulsed regime (350 ps pulses, 1 kHz repetition rate). The fluorescence was spectrally resolved using a single-grating spectrometer (Princeton Instruments Acton SpectraPro 2300i) and acquired by a thermoelectrically cooled Vis CCD camera (AndorNewtonEM). Fluorescence lifetime were measured in air under sample irradiation at 350 nm by an optical parametric amplifier (Light Conversion TOPAS-C) pumped by a regenerative Ti:Sapphire amplifier (Coherent Libra-HE), delivering 200 fs-long pulses at 1 kHz repetition rate. The fluorescence was spectrally dispersed using a single-grating spectrometer (Princeton Instruments Acton SpectraPro2300i) and acquired by a Vis streak camera (Hamamatsu C1091). Pump fluence was kept below 50 μJ cm−2 per pulse in all fluorescence experiments to prevent sample degradation.
Acton spectrapro 2300i
Manufactured by Teledyne
Sourced in United States, Germany
The Acton SpectraPro 2300i is a high-performance spectrometer designed for advanced spectroscopy applications. It features a focal length of 300 mm and provides a wavelength range from 190 nm to 3000 nm. The spectrometer is equipped with a precision-ruled grating and offers multiple grating options to suit various spectroscopic needs.
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Lab products found in correlation
Acton spectrapro 2300i, by Hamamatsu Photonics (2 mentions)
Cary 5000 uv vis nir, by Agilent Technologies (2 mentions)
Spc 130 em, by Teledyne (2 mentions)
Streak camera, by Hamamatsu Photonics (1 mentions)
Bdl 377 smc laser diode, by Becker & Hickl (1 mentions)
F 4500 spectrofluorometer, by Hitachi (1 mentions)
V 670 spectrophotometer, by Jasco (1 mentions)
4 protocols using acton spectrapro 2300i
1
Reflectance and Fluorescence Spectroscopy
2
Optical Characterization of Materials
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Reflectance (R) and transmittance (T) measurements were performed at 8° angle of incidence using a dual-beam spectrophotometer with an integrating sphere accessory (Agilent Cary 5000 UV-Vis-NIR). Absorptance (A) was calculated as 1-R-T.
Time-resolved photoluminescence. Samples were mounted excited by a regenerative amplifier laser (Coherent Libra) delivering 100-fs-long pulses at a repetition rate of 1 KHz. Photoluminescence was dispersed with a grating spectrometer (Princeton Instruments Acton SpectraPro 2300i equipped with a 50 gr/mm grating blazed at 600 nm), dispersed and detected by a streak camera (Hamamatsu).
CW photoluminescence. Samples were excited by a diode-pumped Nd:YVO4 CW laser at 532 nm (Spectra Physics Millennia). Photoluminescence was dispersed by a grating spectrometer (Princeton Instruments Acton SpectraPro 2300i) and detected by a LN-cooled CCD camera (Princeton Instruments PIXIS). At the highest excitation intensities, laser beam was chopped to reduce the overall thermal loading.
Time-resolved photoluminescence. Samples were mounted excited by a regenerative amplifier laser (Coherent Libra) delivering 100-fs-long pulses at a repetition rate of 1 KHz. Photoluminescence was dispersed with a grating spectrometer (Princeton Instruments Acton SpectraPro 2300i equipped with a 50 gr/mm grating blazed at 600 nm), dispersed and detected by a streak camera (Hamamatsu).
CW photoluminescence. Samples were excited by a diode-pumped Nd:YVO4 CW laser at 532 nm (Spectra Physics Millennia). Photoluminescence was dispersed by a grating spectrometer (Princeton Instruments Acton SpectraPro 2300i) and detected by a LN-cooled CCD camera (Princeton Instruments PIXIS). At the highest excitation intensities, laser beam was chopped to reduce the overall thermal loading.
3
Time-Resolved Fluorescence Spectroscopy
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Fluorescence spectra were recorded with a fiber-coupled Ocean Optics QE-Pro FL spectrograph (Dunedin, FL, USA), upon excitation with a picosecond 377 nm BDL-377-SMC laser diode (Becker&Hickl, Berlin, Germany). The fluorescence kinetics was investigated with the use of the time-correlated single-photon counting (TCSPC) method. For TCSPC measurements, we used a Becker&Hickl system (Berlin, Germany) constructed from a TCSPC Module (SPC-130-EM) and a hybrid PMT detector (HPM-100-06) with detector control card (DCC 100) mounted to a spectrograph (Acton SpectraPro-2300i, Princeton Instruments, Trenton, NJ, USA). The sample was excited with a 377 nm BDL-377-SMC picosecond laser diode in the 20 MHz repetition mode (which corresponds to 50 ns collection time window). The fluorescence lifetime values were calculated, after deconvolution procedure of the instrument response function (IRF) which is ca. 200 ps.
4
Characterization of CdS-Au Hybrid Nanoparticles
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The morphology of the synthesized CdS-Au NPs was examined with transmission electron microscopy (TEM) using two electron microscopes: a FEI Tecnai G2 20 X-TWIN microscope with EDX and a PhilipsCM-20 SuperTwin instrument operating at 160 kV. The diluted samples for TEM measurements were ultrasonicated, and a droplet of the suspension was deposited on a TEM dedicated copper grid coated with carbon film.
Initial optical characterization included absorbance and luminescence spectra measurements of the obtained CdS-Au hybrid systems, as well as as-synthesized penicillamine-stabilized CdS NPs along with time-resolved luminescence decay traces. Absorbance measurements were performed with a JASCO V670 spectrophotometer. Luminescence spectra were obtained using a Hitachi F-4500 spectrofluorometer, after excitation at λ = 375 nm. Luminescence lifetimes were measured with a self-constructed time-correlated single-photon counting (TCSPC) Becker & Hickl system (Berlin, Germany), constructed from a TCSPC Module (SPC-130-EM) and a hybrid PMT detector (HPM-100-06) with a detector control card (DCC 100) mounted onto a Princeton Instruments spectrograph (ActonSpectraPro-2300i) under excitation with a picosecond 375 nm laser diode (BDL-375-SMC). The luminescence lifetime values were calculated based on the exponential decay model, with the use of the dedicated Becker & Hickl SPCImage software.
Initial optical characterization included absorbance and luminescence spectra measurements of the obtained CdS-Au hybrid systems, as well as as-synthesized penicillamine-stabilized CdS NPs along with time-resolved luminescence decay traces. Absorbance measurements were performed with a JASCO V670 spectrophotometer. Luminescence spectra were obtained using a Hitachi F-4500 spectrofluorometer, after excitation at λ = 375 nm. Luminescence lifetimes were measured with a self-constructed time-correlated single-photon counting (TCSPC) Becker & Hickl system (Berlin, Germany), constructed from a TCSPC Module (SPC-130-EM) and a hybrid PMT detector (HPM-100-06) with a detector control card (DCC 100) mounted onto a Princeton Instruments spectrograph (ActonSpectraPro-2300i) under excitation with a picosecond 375 nm laser diode (BDL-375-SMC). The luminescence lifetime values were calculated based on the exponential decay model, with the use of the dedicated Becker & Hickl SPCImage software.
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