A complete list of commercially available primary and secondary antibodies used in this study is provided in Supplemental Table 2 . Rabbit anti-MLC1 antibody used for western blot (1:500) and immunochemistry (1:100) was purchased from Novus Biologicals (NBP1–81555). Human-specific goat anti-Vimentin was used for immunohistochemistry (1:40) and was purchased from R&D (AF2105). Rabbit anti-phosphotyrosine pY99 was used for immunofluorescence (1:50) and was purchased from Santa Cruz (sc-7020). Rabbit anti-myelin basic protein (MBP) was used for immunofluorescence (1:200) and was purchased from Abcam (ab40390). Chicken anti-GFP was used for immunofluorescence (1:1000) and was purchased from Aves (GFP-1020). Mouse anti-Myc antibody was used for immunoprecipitation (1μg per 2mg lysate) and western blot (1:2000) and was purchased from Invitrogen (46–0603). Mouse anti-α-Actinin antibody was used for western blot (1:2000) and was purchased from Abcam (ab18061). Mouse anti-GAPDH was used for western blot (1:2000) and was purchased from Sigma (G8796).
Rabbit anti phosphotyrosine py99
Manufactured by Santa Cruz Biotechnology
Rabbit anti-phosphotyrosine pY99 is a primary antibody that specifically recognizes phosphorylated tyrosine residues in proteins. It is commonly used in research applications to detect and study the presence and distribution of tyrosine phosphorylation in biological samples.
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2 protocols using rabbit anti phosphotyrosine py99
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Comprehensive Antibody Usage in Research
2
Comprehensive Antibody Usage in Research
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A complete list of commercially available primary and secondary antibodies used in this study is provided in Supplemental Table 2 . Rabbit anti-MLC1 antibody used for western blot (1:500) and immunochemistry (1:100) was purchased from Novus Biologicals (NBP1–81555). Human-specific goat anti-Vimentin was used for immunohistochemistry (1:40) and was purchased from R&D (AF2105). Rabbit anti-phosphotyrosine pY99 was used for immunofluorescence (1:50) and was purchased from Santa Cruz (sc-7020). Rabbit anti-myelin basic protein (MBP) was used for immunofluorescence (1:200) and was purchased from Abcam (ab40390). Chicken anti-GFP was used for immunofluorescence (1:1000) and was purchased from Aves (GFP-1020). Mouse anti-Myc antibody was used for immunoprecipitation (1μg per 2mg lysate) and western blot (1:2000) and was purchased from Invitrogen (46–0603). Mouse anti-α-Actinin antibody was used for western blot (1:2000) and was purchased from Abcam (ab18061). Mouse anti-GAPDH was used for western blot (1:2000) and was purchased from Sigma (G8796).
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