TG, AG, DHTG, and the corresponding deuterated stable-labeled internal standards TG-d3, AG-d4, and DHTG-d3 were purchased from Cerilliant corporation (Round Rock, TX). EtioG standard was purchased from Steraloids (Newport, RI). Membrane vesicle overexpressing the human BCRP, MDR1, MRP2, MRP3, and MRP4 were provided by Solvo Biotechnology (Budapest, Hungary). Adenosine 5’-triphosphate (ATP) disodium salt, adenosine 5’-monophosphate (AMP) monohydrate, glutathione, Tris-Base (Tris[hydroxymethyl]aminomethane), MgCl2, NaCl, Sucrose, MOPS (3-[N-Morpholino]propanesulfonic acid) were purchased from Sigma-Aldrich (St. Louis, MO). Bovine serum albumin (BSA), membrane protein extraction kit and trypsin digestion reagents were obtained from Thermo Fisher Scientific (Rockford, IL). The synthetic light peptide and heavy labeled peptides were purchased from New England Peptides (Boston, MA) and Thermo Fisher Scientific (Rockford, IL), respectively. Multiscreen™ HTS Vacuum Manifold and 96-well filter plates with class B glass fiber filters were purchased from EMD Millipore (Billerica, MA). Estradiol-17β-glucuronide (E2-17β-G), N-Methyl Quinidine (NMQ), dehydroepiandrosterone sulfate (DHEAS), and estrone-3-sulfate (E3S) were purchased from Sigma-Aldrich (St. Louis, MO). All other chemicals and reagents, unless indicated otherwise, were purchased from Sigma-Aldrich (St. Louis, MO).
96 well filter plates with class b glass fiber filters
Manufactured by Merck Group
96-well filter plates with class B glass fiber filters are laboratory equipment used for sample filtration and preparation. They provide a standardized format with 96 individual wells, each containing a glass fiber filter for efficient sample processing. The core function of these filter plates is to facilitate the filtration and separation of samples, preparatory to further analysis or processing.
Automatically generated - may contain errors
Lab products found in correlation
3 n morpholino propanesulfonic acid mops, by Merck Group (2 mentions)
Sodium chloride (nacl), by Merck Group (2 mentions)
Glutathione (gsh), by Merck Group (2 mentions)
Sucrose, by Merck Group (2 mentions)
Mgcl2, by Merck Group (2 mentions)
Adenosine 5 triphosphate atp disodiumsalt, by Merck Group (2 mentions)
Estrone 3 sulfate, by Merck Group (2 mentions)
Multiscreen hts vacuum manifold, by Merck Group (2 mentions)
Trypsin digestion reagents, by Thermo Fisher Scientific (2 mentions)
Adenosine 5 monophosphate amp monohydrate, by Merck Group (2 mentions)
Tris base, by Merck Group (1 mentions)
Hank s balanced salt solution hbss, by Thermo Fisher Scientific (1 mentions)
Formic acid, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Merck Group (1 mentions)
Glutamax 1, by Merck Group (1 mentions)
Diclofenac, by Merck Group (1 mentions)
Rosuvastatin, by Merck Group (1 mentions)
Furosemide, by Merck Group (1 mentions)
Tris hydroxymethyl aminomethane tris base, by Merck Group (1 mentions)
2 protocols using 96 well filter plates with class b glass fiber filters
1
Membrane Transport Protein Assay Protocol
2
Membrane Vesicle Transport Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Membrane vesicles from
HEK293 cells overexpressing the human BCRP, P-gp, MRP2, MRP3, and
MRP4 as well as the stably transfected hOATP2B1-MDCK-II cells were
provided by Solvo Biotechnology (Budapest, Hungary). Cell culture
medium, i.e., Dulbecco’s modified Eagle’s medium (DMEM),
fetal bovine serum, 1% GlutaMAX-1, 100 U/mL penicillin, and 100 μg/mL
streptomycin were obtained from Sigma-Aldrich (St. Louis, MO). Furosemide,
diclofenac, estrone-3-sulfate, rosuvastatin, adenosine 5′-triphosphate
(ATP) disodium salt, adenosine 5′-monophosphate (AMP) monohydrate,
glutathione, tris[hydroxymethyl]aminomethane (tris-base), MgCl2, NaCl, sucrose, and 3-[N-morpholino] propane
sulfonic acid (MOPS) were purchased from Sigma-Aldrich (St. Louis,
MO). Bovine serum albumin (BSA), membrane protein extraction kit,
and trypsin digestion reagents were obtained from Thermo Fisher Scientific
(Rockford, IL). Hanks’ balanced salt solution (HBSS), phosphate
buffer saline (PBS), acetonitrile, and formic acid were purchased
from Thermo Fisher Scientific (Rockford, IL). The synthetic unlabeled
and stable labeled peptides were purchased from New England Peptides
(Boston, MA) and Thermo Fisher Scientific (Rockford, IL), respectively.
Multiscreen HTS Vacuum Manifold and 96-well filter plates with class
B glass fiber filters were purchased from EMD Millipore (Billerica,
MA).
HEK293 cells overexpressing the human BCRP, P-gp, MRP2, MRP3, and
MRP4 as well as the stably transfected hOATP2B1-MDCK-II cells were
provided by Solvo Biotechnology (Budapest, Hungary). Cell culture
medium, i.e., Dulbecco’s modified Eagle’s medium (DMEM),
fetal bovine serum, 1% GlutaMAX-1, 100 U/mL penicillin, and 100 μg/mL
streptomycin were obtained from Sigma-Aldrich (St. Louis, MO). Furosemide,
diclofenac, estrone-3-sulfate, rosuvastatin, adenosine 5′-triphosphate
(ATP) disodium salt, adenosine 5′-monophosphate (AMP) monohydrate,
glutathione, tris[hydroxymethyl]aminomethane (tris-base), MgCl2, NaCl, sucrose, and 3-[N-morpholino] propane
sulfonic acid (MOPS) were purchased from Sigma-Aldrich (St. Louis,
MO). Bovine serum albumin (BSA), membrane protein extraction kit,
and trypsin digestion reagents were obtained from Thermo Fisher Scientific
(Rockford, IL). Hanks’ balanced salt solution (HBSS), phosphate
buffer saline (PBS), acetonitrile, and formic acid were purchased
from Thermo Fisher Scientific (Rockford, IL). The synthetic unlabeled
and stable labeled peptides were purchased from New England Peptides
(Boston, MA) and Thermo Fisher Scientific (Rockford, IL), respectively.
Multiscreen HTS Vacuum Manifold and 96-well filter plates with class
B glass fiber filters were purchased from EMD Millipore (Billerica,
MA).
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