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2 protocols using perchloric acid

1

Bodenone Decontate and Tramadol Assay

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Bodenone decontate was purchased from Equi-gan® (Lab Tornel, Co., Mexico) and tramadol was obtained from Ministry of Justice, Egypt. Ethanol (90%) (El-Nasr Pharmaceutical chemical company, Egypt), thiobarbituric acid and trichloroacetic acid (Sigma-Aldrich), perchloric acid (Sd fine-chem limited, India),5,5′-Dithiobis(2-nitrobenzoic acid) (Sigma-Aldrich), naphthyl)ethylenediamine dihydrochloride (Sigma), and sulfanilamide (Sigma) were used in the preparation of reagents.
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2

Chitosan-Titanium Composite Preparation

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Titanium metal foil 1 cm × 1 cm with a purity of 99.95% and a thickness of 0.25 mm was purchased from Alfa Aesar, USA. Chitosan (Cs) with medium molecular weight-deacetylated chitin, poly (d-glucosamine), PVA with molecular weight = 89 000–98 000, 99+% hydrolyzed, acetic acid, and platinum foil were purchased from Sigma Aldrich, USA. Perchloric acid (70%) was purchased from SD Fine-Chem Limited, India. Hydrochloric acid (35–38%) was purchased from Alpha Chemika, India. The normal human melanocyte HFB-4 cell line was purchased from American Type Culture Collection. All the biological assessment materials including Dulbecco's Modified Eagle's Medium (DMEM) (purchased from Invitrogen/Life Technologies), Fetal Bovine Serum (FBS), insulin, penicillin streptomycin, MTT, and trypan blue were purchased from Sigma Aldrich and the assay was performed at VACSERA, Egypt.
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