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Fructose is a type of sugar that is commonly found in fruits and honey. It is a monosaccharide, which means it is the simplest form of sugar that can be directly absorbed and used by the body. Fructose is used in various applications, such as in the food and pharmaceutical industries.

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7 protocols using fructose

1

Extraction and Analysis of Chinese Honey

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We ordered formic acid and acetonitrile from Fisher Scientific Inc. (Pittsburgh, PA, USA). We bought fructose, glucose, and sucrose from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Entirely capped chaste (Vitex agnus-castus L.) monofloral honey samples were obtained from Apis mellifera L. colonies in the Miyun District, Beijing, China (latitude, 117.044996°; longitude, 40.440968°) at the end of July 2020. Honey samples were stored at −20 °C until use.
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2

Glucose Oxidase Immobilization Protocol

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Glucose oxidase (GOD, 99%) was procured from Aladdin (Shanghai, China). 3-Aminopropyltriethoxysilane (APTES, 98%) was provided from Beyotime (Shanghai, China). Glucose (99%), fructose (99%), Nacl (99.5%), Kcl (99.5%), cholesterol (98%), and anhydrous ethanol (99.5) were all purchased from Macklin (Shanghai, China). Saccharose (99%), urea (99%), H2SO4 (99.9%), and H2O2 (30%) were offered by Guangzhou Chemical Reagent Factory (Guangzhou, China). PBS (99%) was offered by Xiamen Haibiao Technology Co., Ltd. (Xiamen, China). SMF (G.652.D) was received from YOFC (Wuhan, China). Fetal Bovine Serum (100%) and Dulbecco’s Modified Eagle Medium (100%) were provided by Gibco.
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3

Catalytic Conversion of Biomass

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Microcrystalline cellulose (average particle size of 20 µm, polymerization degree of 250) was purchased from Sigma-Aldrich Co., LLC (St. Louis, MO, USA). Formic acid (FA, 99%), 1,3-dihydroxyacetone (DHA) (98%), glyceraldehyde (Gly, 99%), and acetic acid (AA, 98%) were commercially obtained from the Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Zeolite H-β (Si/Al = 40) was purchased from the Nankai University Catalyst Plant (Tianjin, China). 5-hydroxymethylfurfural (HMF, 98%), erythrose (98%), glucose (99%), fructose (98%), and Bi(NO3)3·5H2O (99%) were purchased from Shanghai Macklin Biochemical Co., Ltd (Shanghai, China). Glycolic acid (GA) (98%), lactic acid (LA) (98%), levulinic acid (LeA) (98%), and glyceric acid (GlyA) (98%) were bought from Shanghai Aladdin Bio-Chem Technology Co., Ltd (Shanghai, China). Other reagents were all in analytical grade and used without further purification.
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4

Formulation of Cellulose-Based Hydrogels

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Commercially available food-grade hydroxypropyl methylcellulose (HPMC) (HT-E15), XG (Mw = 500,000–600,000), GG (Mw = 400,000–600,000), potassium citrate, and calcium chloride were purchased from Shandong Rutocel Co., Ltd. (Taian, China). Sodium alginate (Mw = 98,000; G/M = 1), fructose (Mw = 180.15) and sorbitol were provided by Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, China). Glycerol, beeswax, and PEG were supplied by Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).
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5

Biomass Conversion Chemical Reagents

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Glucose (99.5%), levoglucosan (99.0%), and furfural (99.5%) were purchased from Aladdin Reagent Co. Ltd. (Shanghai, China). HMF (99%, GR), ZrOCl2·8H2O (99.9%), levulinic acid (99.0%), formic acid (99.0%), and fructose (99.0%) were purchased from Macklin Reagent Co. Ltd. (Shanghai, China). NaOH (98%) was purchased from Fuchen Chemical Reagent Co. Ltd. (Tianjin, China). Boric acid (99.5%) was provided by Chinasun Specialty Products Co. Ltd. (Changshu, China). Sulfuric acid (98%) was supplied from Chemical Reagent Co. (Guangzhou, China).
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6

Amino Acid Isolation and Purification

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BSA (fraction V, heat shock isolation) was purchased from Shanghai Sangon Biotech Co., Ltd. (Shanghai, China). AA was obtained from Tianjin Kermel Chem reagent Co., Ltd. (Tianjin, China). Phosphate buffer saline (PBS) was prepared in this experiment. Phe was obtained from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Pro was purchased from the Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Glu, Ser, Asp, Val, Leu, Thr, glucose, sucrose, fructose and GSH were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). All amino acids are L-type. All other chemicals were of analytical grade and used without further purification. The ultrapure water (18.2 MΩ cm) was obtained by the Milli-Q purification system (Milipore, Germany).
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7

Goat Semen Collection and Analysis

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In this experiment, an artificial vagina was used to collect goat semen, and sperm motility analysis was conducted on the collected semen. Six goat semen with sperm motility higher than 80% were mixed, and the mixed semen was diluted eight times. The diluted semen was stored at a constant temperature of 4°C and used for detection and analysis.
The components of semen diluent were as follows: SS 0.16 to 0.8 mg or CC 0.04 to 0.80 mg, vitamin E 4 mL, penicillin sodium 15,000 IU, streptomycin sulfate15,000 IU, which were all bought from Shanghai yuanye Bio-Technology Co., Ltd (Shanghai, China). Fructose 1.26 g and citric acid 1.72 g, which were all bought from Shanghai Macklin Biochemical Co., Ltd (Shanghai, China). Trimethyl aminomethane 3.53 g, vitamin C 0.5 g, bovine serum albumin 0.5 g, which were all bought from Beijing Solarbio Science & Technology Co., Ltd (Beijing, China). Fresh egg yolk 20 mL, double-distilled water up to 100 mL. Diluent components are classified as analytical or guaranteed grade reagents.
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