Analytical HPLC was carried out on Dionex UltiMate HPLC system (Thermo-Fisher, Waltham, MA, USA) consisting of LPG-3400A pump, WPS-3000SL autosampler, and DAD-3000D diode array detector using Gemini-NX C18 column (4.6 × 250 mm, 3 µm, 110 Å).
Preparative HPLC was carried out on an Dionex UltiMate HPLC system consisting of HPG-3200BX pump, Rheodyne 9725i injector, 10 mL loop, MWD-3000 detector, and FCA_Multi automated fraction collector using Gemini-NX C18 (21.2 × 250 mm, 5 µm, 110 Å).
Both analytical (1 mL/min) and preparative (21.2 mL/min) HPLC were performed with gradient elution, 0–100% solvent B (ACN-H2O-TFA 90:10:0.1) in solvent A (H2O-TFA 100:0.1) over 15 min. Data were acquired and processed using the Chromeleon Software version 6.80.
Preparative HPLC was carried out on an Dionex UltiMate HPLC system consisting of HPG-3200BX pump, Rheodyne 9725i injector, 10 mL loop, MWD-3000 detector, and FCA_Multi automated fraction collector using Gemini-NX C18 (21.2 × 250 mm, 5 µm, 110 Å).
Both analytical (1 mL/min) and preparative (21.2 mL/min) HPLC were performed with gradient elution, 0–100% solvent B (ACN-H2O-TFA 90:10:0.1) in solvent A (H2O-TFA 100:0.1) over 15 min. Data were acquired and processed using the Chromeleon Software version 6.80.