Vimentin rabbit mab

Manufactured by Cell Signaling Technology

Sourced in United States

Vimentin rabbit mAb is a monoclonal antibody that specifically recognizes the vimentin protein. Vimentin is a type III intermediate filament protein that is expressed in various cell types, including mesenchymal cells and some epithelial cells. This antibody can be used for the detection and analysis of vimentin in various applications, such as Western blotting, immunohistochemistry, and immunocytochemistry.

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Lab products found in correlation

E cadherin rabbit mab, by Cell Signaling Technology (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) N cadherin rabbit mab, by Cell Signaling Technology (1 mentions) Facscalibur system, by BD (1 mentions) Hrp linked anti mouse igg, by Cell Signaling Technology (1 mentions) Yap rabbit mab, by Cell Signaling Technology (1 mentions) Gapdh mouse mab, by Abmart (1 mentions)

Close spelling variants of this product

Vimentin XP Rabbit mAb Vimentin (D21H3) XP® Rabbit mAb Anti-Vimentin rabbit mAb Rabbit mABs Vimentin

4 protocols using vimentin rabbit mab

Malignant Glioma Cell Lines Protocol

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Two human malignant glioma cell lines (U87‐MG, SNB19) were purchased from the Institute of Biochemistry and Cell Biology (Shanghai, China) and were cultured in Dulbecco modified Eagle medium (Gibco, Invitrogen Inc., Carlsbad, CA) supplemented with 10% fetal bovine serum (Gibco) and incubated at 37℃ in 5% CO₂. NKCC1 rabbit mAb (No.ab59791 WB 1:1,000, IHC 1:100), NKCC1 goat mAb (No.ab99558 IF 1:100), vinculin mouse mAb (No.ab129002 IF 1:100), MMP‐2 rabbit mAb (No.ab37150 WB 1:1000) were purchased from Abcam (UK), E‐cadherin ribbit mAb (No.3195P WB 1:1,000), N‐cadherin rabbit mAb (No.13116S WB 1:1,000 IF 1:100), snail rabbit mAb (No.3879P WB 1:1,000 IF 1:100), vimentin rabbit mAb (No.5741P WB 1:1,000 IF 1:100) were obtained from Cell Signaling Technology (Danvers, MA). B‐actin mouse mAb (15V70207 WB 1:200‐1,000), GADPH mouse mAb (TA309157 WB 1:2,000) was obtained from ZSGB‐BIO (China).
NKCC1 inhibitor bumetanide (BMT), Rac1 inhibitor NSC25766, RhoA inhibitor Y27632 from Selleck (China). Epidermal growth factor (EGF) cytokines for cell activated were purchased from Gibco.

Ma H., Li T., Tao Z., Hai L., Tong L., Yi L., Abeysekera I.R., Liu P., Xie Y., Li J., Yuan F., Zhang C., Yang Y., Ming H., Yu S, & Yang X. (2018). NKCC1 promotes EMT‐like process in GBM via RhoA and Rac1 signaling pathways. Journal of Cellular Physiology, 234(2), 1630-1642.

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Flow Cytometric Analysis of EMT in NCCs

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EMT in NCCs was analyzed by flow cytometry. NCCs were harvested, fixed with 4% paraformaldehyde, and permeabilized with 0.1% Triton X-100 in phosphate-buffered saline (PBS) for 20 min at room temperature. After that, the cells were blocked by 5% goat serum in PBS for 30 min and then incubated with E-cadherin Rabbit mAb (PE conjugate) (Cell Signaling, Beverly, MA, USA), Vimentin Rabbit mAb (Cell Signaling, Beverly, MA, USA) for 30 min at 37°C, and then incubated with fluorescein isothiocyanate (FITC)-labeled goat anti-rabbit IgG secondary antibody (Abcam, Cambridge, MA, USA) for 30 min at 37°C. Flow cytometry analysis was performed on a BD FACSCalibur system. The average fluorescence intensity was analyzed using FlowJo 7.6.1 software.

Fan H., Li Y., Yuan F., Lu L., Liu J., Feng W., Zhang H.G, & Chen S.Y. (2022). Up-regulation of microRNA-34a mediates ethanol-induced impairment of neural crest cell migration in vitro and in zebrafish embryos through modulating epithelial-mesenchymal transition by targeting Snail1. Toxicology letters, 358, 17-26.

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Peptide-Hydrogel Scaffold for Cell Culture

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4-arm poly(ethylene glycol)-amine (20 kDa) was purchased from JenKem Technology USA. Reagents and chemicals for peptide synthesis were were acquired from Anaspec or Chempep. Bovine type I collagen was purchased from Amsbio. AlamarBlue reagents were purchased from AbD Serotec. Live/Dead staining kit for mammalian cells and DAPI stain were obtained from Invitrogen. Gemcitabine was purchased from TSZ CHEM. YAP rabbit mAb, E-cadherin rabbit mAb, vimentin rabbit mAb, anti-rabbit IgG, anti-mouse IgG HRP-linked, and Alexa Fluor® 488-labeled anti-mouse IgG F(ab’)₂ antibodies were obtained from Cell Signaling Technology. hVEGF ELISA kit was purchased from PeproTech. HPLC grade acetonitrile and water were acquired from Fisher Scientific and VWR International, respectively. All other chemicals were purchased from Sigma-Aldrich unless noted otherwise.

Ki C.S., Lin T.Y., Korc M, & Lin C.C. (2014). Thiol-ene hydrogels as desmoplasia-mimetic matrices for modeling pancreatic cancer cell growth, invasion, and drug resistance. Biomaterials, 35(36), 9668-9677.

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Investigating miR-7 Regulation of EGFR Signaling

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MiR-7 plasmid and negative control (NC) were synthesized by Shanghai IBS Company. The sequence of plasmid and NC are as follows: 5′-UGGAAGACUAGUGAUUUUGUUGU-3′; Negative Control: 5′-GAAATCTACTGCGCGTGGAGAC-3′ (IBS company). TaqMan kit (Applied Biosystems) specified for quantification of miRNA was used to assess the expression of miR-7 and U6. EGF receptor Rabbit mAb (# 4267), Phospho-EGF Receptor (Tyr992) Rabbit mAb (#2235), Phospho-p44/42 MAPK (Thr202/Tyr204) Rabbit mAb (#4370), Phospho-Akt (Ser473/Thr308), Rabbit mAb (#4060/#2965), Akt (pan) Rabbit mAb (#4691), LY294002 (PI3K Kinase Inhibitor) (#9901), U0126 (MEK1/2 Inhibitor) (#9903), Vimentin Rabbit mAb (# 5741), and β-catenin rabbit mAb and E-cadherin Rabbit mAb (# 3195) were purchased from Cell Signaling Technology (CST). cytokeratin-18 (CK-18) mAb (T410) pAb (BS1204) was purchased from Bioworld Technology. P44/42 MAPK Rabbit mAb was obtained from Santa Cruz ((sc-33746)). GAPDH mouse mAb was purchased from ABmart (#M20006). 800CW conjugated goat anti-Rabbit IgG((926-32210), highly cross adsorbed and 800CW conjugated goat anti-mouse IgG(926-32211), highly cross adsorbed were purchased from Li-Cor Biosciences. EGFR and control short interfering RNAs (siRNA) were from Sanong Biotech.Has-miR-7-LNA detection probe were purchased from Exiqon (38485-15).

Zhou X., Hu Y., Dai L., Wang Y., Zhou J., Wang W., Di W, & Qiu L. (2014). MicroRNA-7 Inhibits Tumor Metastasis and Reverses Epithelial-Mesenchymal Transition through AKT/ERK1/2 Inactivation by Targeting EGFR in Epithelial Ovarian Cancer. PLoS ONE, 9(5), e96718.

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