Rabbit anti kif2a

Primary antibodies used included rabbit anti-Kif2a (1: 1000, Abcam), rabbit anti-Tbr2 (1:500, Abcam), rabbit anti-Pax6 (1:200, Covance), mouse anti-beta III tubulin (TU20) (1:500, Abcam), mouse anti-nestin (1:500, Abcam), mouse anti-α tubulin (1:5000, CST), rat anti-Brdu (1:500, Abcam), rabbit and chicken anti-green fluorescent protein (1:500, GFP) (Invitrogen and Aves, respectively), rabbit anti-Ki67 (1:500, Thermo), rabbit anti-PH3 (1:300, Millipore), rabbit anti-cleaved caspase3 (1:300, Cell signaling), rabbit anti-AKT (1:500, CST), rabbit anti-p-AKT (1:500, CST), rabbit anti-β-catenin (1:200, Santa Cruz), mouse anti-GSK3β (1:200, Santa Cruz), and mouse anti-p-GSK3β (1:200, Santa Cruz). All the corresponding conjugated secondary antibodies were purchased from Invitrogen. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (Roche).