Control probe

Manufactured by Thermo Fisher Scientific

The Control Probe is a laboratory instrument designed to provide a reliable reference signal for calibration and quality control purposes. It functions as a stable and consistent source of a specific signal or response, allowing users to validate the performance and accuracy of their measurement equipment. The Control Probe enables researchers and technicians to ensure the proper functioning of their analytical instruments, helping to maintain the integrity of their experimental data and research findings.

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Lab products found in correlation

Dynabead, by Thermo Fisher Scientific (1 mentions) Wash buffer, by Thermo Fisher Scientific (1 mentions) Control probe, by Genechem (1 mentions) Streptavidin coated magnetic beads, by Promega (1 mentions) Cell lysates, by Promega (1 mentions)

Spelling variants of this product

TaqMan Ribosomal RNA Control Reagent VIC probe (5 citations) 18S rRNA endogenous control probe (4 citations) Eukaryotic 18S rRNA endogenous control probe (4 citations) 18S rRNA endogenous control primer/probe set (2 citations) TaqMan Ribosomal RNA Control Reagents VIC Probe (2 citations) Eukaryotic 18 S rRNA Endogenous Control (VIC™/MGB probe, (2 citations) Ribosomal RNA Control Reagents (VIC probe) (2 citations) Mouse GAPDH Endogenous Control VIC Probe (2 citations) Human GAPDH Endogenous Control FAM™ Dye/MGB Probe (2 citations) VIC-labeled TaqMan genomic control probe (RNAseP; (2 citations)

2 protocols using control probe

Biotinylated LINC00461 Pulldown Assay

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Biotinylated LINC00461 and a control probe were obtained from Genechem Biotech (Shanghai, China). Cell lysates were incubated with LINC00461 or the control probe and streptavidin-coupled Dynabeads (Invitrogen) to generate probe-bound Dynabeads. After washing using wash buffer (Invitrogen), RNA complexes were isolated and purified using lysis buffer and proteinase K. Purified RNA was analyzed by qRT-PCR.

Zhang C., Wang J., Zhang J., Qu H, & Tang X. (2020). LINC00461 Overexpression Can Induce Docetaxel Resistance in Breast Cancer by Interacting with miR-411-5p. OncoTargets and therapy, 13, 5551-5562.

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Identification of miR-1245b-5p Targets

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Biotinylated probes with wild-type or mutated miR-1245b-5p (Bio-miR-1245b-5p-WT/Mut) or control probe (Bio-NC) were synthesized (Invitrogen, Shanghai). The biotin-labeled RNAs were incubated with streptavidin-coated magnetic beads and the cell lysates (Promega, Madison, WI, USA) at 4°C for 4 h. The bound RNAs were purified through the washing of beads and extracted with TRIzol reagent. Finally, the RNAs were detected through qRT-PCR analysis.

Zhang W., Liu Q., Zhao J., Wang T, & Wang J. (2021). Long Noncoding RNA AATBC Promotes the Proliferation and Migration of Prostate Cancer Cell Through miR-1245b-5p/CASK Axis. Cancer Management and Research, 13, 5091-5100.

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