Quantikine hs high sensitivity elisa kit

Venous blood samples were taken at the time of scanning. Five millilitres were assayed on the same day for high sensitivity C-Reactive Protein (hsCRP), using a standard immunoturbidimetric method on an Abbott c 16 000 automatic chemistry analyzer (Abbott Diagnostics, Maidenhead, UK). Another 5 mL were collected into an EDTA tube, centrifuged for 10 min at 1250×g within 30 min to separate plasma, which was then stored at −30 °C until assay. On study completion, the plasma samples were transferred in a single batch, in unlinked anonymized form, for Human IL-6 Immunoassay using a Quantikine® HS (high sensitivity) ELISA kit (R&D Systems, Abingdon, UK). The assay was performed in duplicates.
Salivary cortisol was assayed in duplicate using a commercial, ELISA-based method according to manufacturer’s instructions (Salimetrics).

Five milliliter blood samples were collected between February 16, 2015 and July 6, 2015 into an EDTA tube 2 h post injection, centrifuged for 10 min at 1,250×g within 30 min. Plasma was then stored at −30 °C until assay on September 23, 2015. On study completion, the plasma samples were transferred in a single batch, in unlinked anonymised form, for Human IL-6 Immunoassay using a Quantikine® HS (high sensitivity) ELISA kit (R&D Systems, Abingdon, UK). The intra-assay and inter-assay coefficients of variation are 7.4 and 7.8 %, respectively, and the limit of detection of the assay is 0.039 pg/mL.