Naproxen was obtained from Tokyo Chemical Industry Co (Japan). Other excipients used to prepare the liqui-pellet included microcrystalline cellulose (Avicel PH-101), (FMC corp., UK), hydrophilic fumed silica with specific surface area of 300 m2/g (Aerosil 300) (Evonik Industries AG, Hanau, Germany), sodium starch glycolate Type A (Primojel) (DFE Pharma, Goch, Germany), croscarmellose (Primellose) (DFE Pharma., Goch, Germany), 2-propanol (VWR Chemicals, Fontenay Sous Bois France), polysorbate 80 (Tween 80) and PEG with molecular weight of 1500 (Acros Organics, UK). All other reagents and solvent were of analytical grades.
Polysorbate 80 tween 80
Manufactured by Thermo Fisher Scientific
Sourced in Germany, United States, Italy
Polysorbate 80 (Tween® 80) is a non-ionic surfactant commonly used as an emulsifier and stabilizer in various laboratory and industrial applications. It is a viscous, yellow liquid with a mild odor. Polysorbate 80 is known for its ability to solubilize, emulsify, and stabilize a wide range of substances, including aqueous and non-aqueous solutions.
Automatically generated - may contain errors
Lab products found in correlation
Beeswax, by Carl Roth (3 mentions)
Aerosil 300, by Evonik (2 mentions)
Alloxan, by Merck Group (2 mentions)
Glucophage, by Merck Group (2 mentions)
Sorbitan monostearate, by Merck Group (2 mentions)
Poly ε caprolactone pcl mn 45000, by Merck Group (2 mentions)
Water chromasolv plus for hplc, by Honeywell (2 mentions)
2 propanol, by Avantor (1 mentions)
Citrate buffer, by Teknova (1 mentions)
Squalene oil, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Coartem, by Novartis (1 mentions)
Propylene glycol, by Thermo Fisher Scientific (1 mentions)
Naproxen, by Tokyo Chemical Industry (1 mentions)
Macrogolglycerol ricinoleate 35 kolliphor el, by BASF (1 mentions)
Caprylocaproyl macrogol 8 glycerides, by Gattefosse (1 mentions)
Strat m, by Merck Group (1 mentions)
Supor pes membranes, by Pall Corporation (1 mentions)
Squalene, by Merck Group (1 mentions)
Hmgb1, by Merck Group (1 mentions)
11 protocols using polysorbate 80 tween 80
1
Naproxen Liqui-Pellet Formulation
2
Squalene Oil-based Emulsion Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Squalene oil, sorbitane trioleate (Span 85) and phosphate buffered saline (PBS) were obtained from Sigma Aldrich and polysorbate 80 (Tween 80) from Acros Organic. Millipore MilliQ deionized water was used and citrate buffer was acquired from Teknova. Chromatography column was obtained from Waters: Acquity UPLC (R) BEH C18 1.7 µm 2.1 × 50 mm.
3
Metformin-Loaded Topical Formulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The pure sample of metformin used was obtained as a gift from May and Baker PLC (Ikeja, Lagos State, Nigeria). Phospholipon® 90H (P90H) (Phospholipid GmbH, Köln, Germany), sorbitol (Caesar & Loretz, Hilden, Germany), sorbic acid (Foodchem Int. Co., China), polyethylene glycol 4000 (PEG 4000) (Ph. Eur. Carl Roth GmbH + Co. KG Karlsruhe, Germany), beeswax (Carl Roth, Karlsruhe, Germany), Polysorbate 80 (Tween® 80) (Acros Organics, Geel, Belgium), Capryol-PGE 860 (Gattefossé, Saint – Priest Cedex, France), Glucophage® purchased from model pharmacy University of Nigeria, Nsukka (Merck KGaA, Darmstadt, Germany), Alloxan (Merck KGaA, Darmstadt, Germany) and double distilled water (Lion water, University of Nigeria, Nsukka, Nigeria) and other solvents and reagents were used as procured from their manufacturers without further purification. Adult albino Wistar rats of both sexes were procured from the Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Nigeria.
4
Artemether-Lumefantrine Nanoemulsion Formulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Artemether was obtained from Hangzhou Dayang Chemical Co. Ltd. (Zhejiang, China), while lumefantrine was sourced from Guilin Pharmaceutical Co Ltd. (Shanghai, China). Phospholipon® 90H (P90H) (Phospholipid GmbH, Köln,, Germany), sorbitol (Caesar &Loretz, Hilden, Germany), sorbic acid (Foodchem Int. Co., China), beeswax (Carl Roth, Karlsruhe, Germany), Polysorbate 80 (Tween® 80) (Acros Organics, Geel, Belgium), Capryol-PGE 860 (Abitech Corp, USA), Coartem® (Novartis, Basel, Switzerland) and distilled water (Lion water, University of Nigeria, Nsukka, Nigeria) and other solvents and reagents were used as procured from their manufacturers without further purification.
Adult albino Wistar mice of both sexes infected with Plasmodium berghei(NK 65) malaria parasites were employed in the study.
Adult albino Wistar mice of both sexes infected with Plasmodium berghei(NK 65) malaria parasites were employed in the study.
5
Liqui-Pellet Formulation of Naproxen
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Naproxen was obtained from Tokyo Chemical Industry Co (Japan). Other excipients used to prepare the liqui-pellet included microcrystalline cellulose (Avicel PH-101), (FMC Corp., UK); colloidal silicon dioxide (Aerosil 300), (Evonik Industries AG, Hanau, Germany); polyethylene glycol 200 (Fisher Scientific, Leicester, UK); propylene glycol (SAFC, Spain); polysorbate 80 (Tween 80), (Acros, Netherlands); linoleoyl macrogol-6 glycerides (Labrafil), (Gattefosse, Saint Priest, France); caprylocaproyl macrogol-8 glycerides (Labrasol), (Gattefosse, Saint Priest, France); and macrogolglycerol ricinoleate 35 (Kolliphor EL), (BASF SE, Ludwigshafen, Germany). All other reagents and solvent were of analytical grades.
6
Metformin-loaded Lipid Nanoparticle Formulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Metformin hydrochloride pure sample was obtained as a gift from May and Baker PLC (Ikeja, Lagos State, Nigeria). Phospholipon® 90H (P90H) (Phospholipid GmbH, Köln, Germany), sorbitol (Caesar & Loretz, Hilden, Germany), polyethylene glycol 4000 (PEG 4000) (Ph. Eur. Carl Roth GmbH + Co. KG Karlsruhe, Germany), beeswax (Carl Roth, Karlsruhe, Germany), Polysorbate 80 (Tween® 80) (Acros Organics, Geel, Belgium), Alloxan (Merck KGaA, Darmstadt, Germany), Glucophage® (Merck), distilled water (Lion water, University of Nigeria, Nsukka, Nigeria) and other solvents and reagents were used as procured from their manufacturers without further purification. Adult albino Wistar rats of both sexes were procured from the Faculty of Veterinary Medicine, University of Nigeria, Nsukka.
7
PAMPA Permeability Assay of CBD
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CBD (purity 99.46%) was obtained from DB Labs (Las Vegas, NV, USA). Materials used for the PAMPA experiments including PAMPA sandwich, buffers, and reference compounds including warfarin, piroxicam, verapamil, and progesterone were purchased from Pion Inc. (Billerica, MA, USA). Cellulose and Strat-M® membranes were purchased from Millipore Sigma (Burlington, MA, USA). Supor PES membranes (pore size 0.1 and 0.45 μM) were purchased from Pall Corporation (Port Washington, NY, USA). Silky Cream Base (BAS-SLKC-01), natural gel base (BAS-NATGE-01), polyisobutene (PIB), mineral oil, phytosqualane, and propylene glycol were purchased through MakingCosmetics (Redmond, WA, USA). Polysorbate 20 NF (Tween 20) was purchased from Letco Medical (Decatur, AL, USA). Polysorbate 80 (Tween 80) was purchased through Fisher Scientific (Waltham, MA, USA). Sodium lauryl sulfate (SLS) was purchased from Gallipot (St. Paul, MN, USA). Simugel and phytosqualane were kindly provided by Seppic (Fairfield, NJ, USA).
8
Development of Nanocapsules with Essential Oils
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All solvents were of analytic grade. In order to prepare the essential oil-loaded nanocapsules, sorbitan monostearate (SM) and poly(ε-caprolactone) (PCL) (Mn 45000) were obtained from Sigma-Aldrich (Milan, Italy) and polysorbate 80 (Tween 80) was obtained from Fisher Chemical (Fisher Scientific, Geel, Belgium). Water Chromasolv Plus for HPLC (Honeywell Riedel-de-Haën, Seelze, Germany) was used. Commercial essential oils of oregano and thyme were provided by Esperis S.p.A., (Milan, Italy) and by Flora s.r.l. (Lorenzana, Pisa, Italy), respectively. A standard mix of n-alkanes C9–C22 was purchased by Alltech (Italy).
9
Formulation and Characterization of Squalene-Based Adjuvants
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Squalene was obtained from Sigma-Aldrich (St. Louis, MO, USA). 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC) was purchased from Avanti Polar Lipids (Birmingham, AL). Resiquimod (R848) was purchased from MedChem Express (Monmouth Junction, NJ, USA), and SD-101 was synthesized by Alpha DNA (Montreal, QC, Canada). TNFa, IL-6, and IL-12p70 mouse uncoated ELISA kits and high-capacity cDNA reverse transcription kit were purchased from Invitrogen (Waltham, MA, USA). SsoAdvancedTM Universal SYBR® Green Supermix was purchased from Bio-Rad Laboratories (Hercules, CA, USA). Real-time PCR pre-designed primers for murine Pdl1 (Cd274), Calreticulin, Hmgb1, and Actb were obtained from Sigma-Aldrich (St. Louis, MO, USA). Primers for murine Cd3e, CD4, CD8a, Foxp3, and Ifnγ were designed (Table S1 ) and synthesized by ThermoFisher Scientific (Waltham, MA). Polysorbate 80 (Tween 80) and all other chemicals and buffers otherwise stated were purchased from Fisher Scientific (Hampton, NH, USA).
10
Paclitaxel Release and Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PTX was purchased from Biorbyt Ltd, Cambridge, Cambridgeshire, United Kingdom. Slide-A-Lyzer™ cassette (MWCO of 10 kDa) was obtained from Thermo-Fisher Scientific. HEPES was purchased from Sigma Aldrich (Saint Louis, USA). Analytical grade ethanol was purchased from Merck (Darmstadt, Germany) and HPLC-S gradient grade acetonitrile was obtained from Biosolve Chimie SARL (Dieuze, France). Polysorbate 80 (Tween 80®, Fisher Scientific UK, Loughborough, Leicester, UK) and Bovine Serum Albumin (Sigma A-4503) were used as solubilizers of released PTX in the release tests. Spectra/Por® Float-A-Lyzer® G2 dialysis membranes with the MWCO of 100 kDa and 300 kDa were purchased from Spectrum labs (Rancho Dominguez California, USA). All cell labeling reagents and fluorescent dyes were purchased from Thermo Fisher Scientific (Naarden, the Netherlands) unless stated otherwise. Cell medium and supplements were from Gibco BRL Thermo Fisher Scientific (Naarden, the Netherlands).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!