Proteins were extracted with radioimmunoprecipitation (RIPA) lysis buffer with PMSF and quantified using a BCA protein assay kit (Beyotime). Western blotting was performed in triplicates according to a previously described protocol [23 (link)]. The following exosomal protein antibodies were used: CD63 (1:500, rabbit IgG; Proteintech, 25682-1-AP), HSP90 (1:1000, rabbit IgG; Proteintech, 13171-1-AP), calnexin (1:2000, rabbit IgG; Cell Signaling Technology, 2433s), horseradish peroxidase (HRP)-conjugated anti-rabbit-IgG, and HRP-conjugated anti-mouse-IgG (NeoBioscience).
Hrp conjugated anti mouse igg
Manufactured by Neobioscience
HRP-conjugated anti-mouse-IgG is a secondary antibody that binds to mouse immunoglobulin G (IgG) and is conjugated with horseradish peroxidase (HRP). It is commonly used in various immunoassays and detection techniques to identify the presence and/or quantity of mouse IgG in samples.
Automatically generated - may contain errors
Lab products found in correlation
Bca protein assay kit, by Beyotime (3 mentions)
Horseradish peroxidase hrp conjugated anti rabbit igg, by Neobioscience (2 mentions)
Hsp90, by Proteintech (1 mentions)
Calnexin, by Cell Signaling Technology (1 mentions)
Phenyl methyl sulfonyl fluoride (pmsf), by Beyotime (1 mentions)
Lmnb1, by Proteintech (1 mentions)
Ab32043, by Proteintech (1 mentions)
Ab76299, by Abcam (1 mentions)
3 protocols using hrp conjugated anti mouse igg
1
Exosomal Protein Characterization by Western Blot
2
Western Blot Analysis of Cell Cycle Regulators
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Protein was extracted using RIPA buffer with PMSF (1:100, Beyotime, China) and was quantified with BCA Protein Assay kit (Beyotime, China). Western blotting was performed in triplicates as previously described [33 (link)]. The following antibodies were used: P16 (1:1000, rabbit IgG; Proteintech, 10883-1-AP), P21 (1:1000, rabbit IgG; Proteintech, 10355-1-AP), P53 (1:1000, rabbit IgG; Proteintech, 10442-1-AP), LMNB1 (1:1000, mouse IgG; Proteintech, 66095-1-Ig). Horseradish peroxidase (HRP)-conjugated anti-rabbit-IgG, and HRP-conjugated anti-mouse-IgG (NeoBioscience).
3
Comprehensive Protein Analysis by Western Blot
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Proteins were extracted with radioimmunoprecipitation (RIPA) lysis buffer with PMSF, quantified by BCA Protein Assay Kit (Beyotime). Western blot was performed in triplicates according to the procedures reported previously [24 (link)]. GAPDH was used as an internal control. We used the following antibodies: p-AKT (1:2000; rabbit IgG, CST, 4060T), p-ERK1/2 (1:5000; rabbit IgG, Abcam, ab76299), p-STAT5α (1:1000; rabbit IgG, Abcam, ab30648), p-GSK (1:5000; rabbit IgG, Abcam, ab75814), AKT (1:1000; mouse IgG, proteintech, 60203-2-Ig), ERK1/2 (1:1000; rabbit IgG, proteintech, 16443-1-AP), STAT5α (1:1000; rabbit IgG, Abcam, ab32043), GSK3β (1:1000, rabbit IgG, proteintech, 22104-1-AP), CD63 (1:500; rabbit IgG, proteintech, 25682-1-AP), TSG101 (1:500; rabbit IgG, Abcam, ab83), HSP70 (1:100; rabbit IgG, SBI, EXOAB-KIT-1), calnexin (1:2000; rabbit IgG, CST, 2433s), GAPDH (1:10000; rabbit IgG, proteintech, 10494-1-AP) (1:10000; mouse IgG, proteintech, 60004-1-Ig), HRP-conjugated anti-rabbit-IgG (NeoBioscience), HRP-conjugated anti-goat-IgG (NeoBioscience), and HRP-conjugated anti-mouse-IgG (NeoBioscience).
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