Mouse anti myc clone 9e10

Manufactured by Thermo Fisher Scientific

The Mouse anti-MYC (clone 9E10) is a monoclonal antibody that specifically recognizes the c-Myc protein. It is a useful tool for the detection and analysis of c-Myc expression in various biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Alexa fluor dye, by Thermo Fisher Scientific (1 mentions) Rabbit anti v5, by Abcam (1 mentions) Chicken anti ha, by Abcam (1 mentions) Rabbit anti h3k4me3, by Active Motif (1 mentions)

Spelling variants of this product

Anti-Myc (70 citations) Mouse anti-myc (12 citations) Anti-9E10 anti-myc (2 citations) Mouse monoclonal anti c-Myc (clone 9E10) (2 citations)

2 protocols using mouse anti myc clone 9e10

Immunofluorescence Imaging of Meiotic Nuclei

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Meiotic nuclei were surface spread on glass slides and imaged as described in [13 (link)]. The following antibodies were used: rabbit anti-Zip1 (created as described in [10 (link)]), rabbit anti-Red1 [61 (link)], guinea pig anti-SUMO [11 (link)], chicken anti-HA (Abcam), mouse anti-MYC (clone 9E10, Invitrogen), rabbit anti-V5 (Abcam). Secondary antibodies conjugated with Alexa Fluor dyes were purchased from Life Technologies and used at a 1:200 dilution.

Voelkel-Meiman K., Johnston C., Thappeta Y., Subramanian V.V., Hochwagen A, & MacQueen A.J. (2015). Separable Crossover-Promoting and Crossover-Constraining Aspects of Zip1 Activity during Budding Yeast Meiosis. PLoS Genetics, 11(6), e1005335.

+ Open protocol

+ Expand

Yeast Lysate Preparation and Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols

Yeast lysates were prepared by 0.2 M NaOH treatment of harvested cells (Kushnirov 2000 (link)), followed by SDS-PAGE and transferred to PVDF. The following primary antibodies were used: rabbit anti-H3K4me3 (catalog no. 39159; Active Motif), rabbit anti-H3K4me2 (catalog no. 39141; Active Motif), rabbit anti-H3 (catalog no. 39163; Active Motif), mouse anti-MYC (clone 9E10, catalog no. MA1980; Invitrogen). A Licor C-DiGit Chemiluminescent Western Blot Scanner was used for imaging.

Jaiswal D., Jezek M., Quijote J., Lum J., Choi G., Kulkarni R., Park D, & Green E.M. (2017). Repression of Middle Sporulation Genes in Saccharomyces cerevisiae by the Sum1-Rfm1-Hst1 Complex Is Maintained by Set1 and H3K4 Methylation. G3: Genes|Genomes|Genetics, 7(12), 3971-3982.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!