Rabbit anti blbp

Manufactured by Merck Group

Sourced in United Kingdom

Rabbit anti-BLBP is a laboratory reagent used in various research applications. It is an antibody that specifically binds to the Brain Lipid-Binding Protein (BLBP), a protein expressed in the central nervous system.

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Rabbit anti- (2 citations)

4 protocols using rabbit anti blbp

Comprehensive Immunostaining and In Situ Hybridization

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Immunostaining and in situ hybridization were performed as previously described [47 (link)]. The Fezf2 cRNA probe corresponds to nucleotides 644 to 1,374 of mouse Fezf2 (GenBank: NC_000080). LacZ staining was preformed according to standard protocols. Primary antibodies used: Chicken anti β-gal (Abcam, 1:500), Rabbit anti BLBP (Millipore, 1:500), Rat anti CTIP2 (Abcam, 1:1,000), Guinea Pig anti GFAP (Advaned Immuno Chemical, 1:100), Rabbit anti PAX6 (Covance, 1:100), PHH3 (Cell Signaling, 1:100), Rabbit anti SATB2 (Abcam, 1:1,000), Goat anti SOX2 (Santa Cruz Biotech, 1:500), Rabbit anti SOX5 (Abcam, 1:500), Rabbit anti TBR1 (Abcam, 1:1,000), Mouse anti Tuj1 (Covance, 1:1,000). Primary antibodies were detected using AlexaFluor-conjugated secondary antibodies (Invitrogen, 1:1,000). DNA was visualized with DAPI (1:50,000).

Eckler M.J., Larkin K.A., McKenna W.L., Katzman S., Guo C., Roque R., Visel A., Rubenstein J.L, & Chen B. (2014). Multiple conserved regulatory domains promote Fezf2 expression in the developing cerebral cortex. Neural Development, 9, 6.

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Immunofluorescence Staining of Embryonic Tissue

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Embryos were fixed in 4% formaldehyde in phosphate buffer (PB) for 2 h at room temperature or overnight at 4°C. For interneuron and sensory neuron staining, embryos were fixed in a solution of 4% formaldehyde, 0.05% glutaraldehyde, 5 mM EGTA, 5 mM MgSO₄, and 0.1% Triton-X in PB for 1 h at room temperature (Dekens et al., 2003 (link)). Whole mount or section immunofluorescence was conducted as previously described (Johnson et al., 2014 (link)). Tissue sections were collected at 0.14 μm with a Leica cryostat. Primary antibodies used include rabbit anti-Gfap (1:500, Dako), mouse anti-Zrf1 (Gfap; 1:100, ZIRC), rabbit anti-Sox2 (1:500, Abcam), rabbit anti-Blbp (1:300, Millipore, ABN14), rabbit anti-GFP (1:300, Invitrogen), rabbit anti-activated caspase-3 (1:500, BD Pharmingen), mouse anti-acetylated tubulin (AT; 1:800, Sigma), rat anti-BrdU (1:100, AbD Serotec), rabbit anti-GABA (1:1000, Sigma), and mouse anti-Islet-1 (39.4D5, 1:200, DSHB). Nuclei were visualized in sectioned tissue with Hoechst stain (1:30,000, Invitrogen).

Johnson K., Barragan J., Bashiruddin S., Smith C.J., Tyrrell C., Parsons M.J., Doris R., Kucenas S., Downes G.B., Velez C.M., Schneider C., Sakai C., Pathak N., Anderson K., Stein R., Devoto S.H., Mumm J.S, & Barresi M.J. (2016). Gfap-Positive Radial Glial Cells Are an Essential Progenitor Population for Later-Born Neurons and Glia in the Zebrafish Spinal Cord. Glia, 64(7), 1170-1189.

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Immunohistochemical Analysis of Cerebral Organoids

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Cerebral organoids were fixed in 4% paraformaldehyde and incubated with sucrose gradient solutions (10, 20 and 30% in PBS) for 15 min each. They were then embedded in optimal cutting temperature compound (OCT) and frozen in liquid nitrogen. Twenty-micron-thick sections were permeabilized with 0.3% Triton-X (Vetec Química Fina Ltda, Rio de Janeiro, Rio de Janeiro, BR) for 5 min at room temperature. Subsequently, the tissue sections were blocked with 3% bovine serum albumin (BSA, Sigma) and 5% normal goat serum (NGS, Invitrogen) in PBS (block solution) for 1 h and incubated overnight at 4 °C with the specified primary antibody diluted in blocking solution. The primary antibodies used were mouse anti-Nestin (1:200; Merck), rabbit anti-BLBP (1:200; Millipore), and rabbit anti-FOXG1 (1:00; Santa Cruz Biotechnology, Inc.). Secondary antibodies were Alexa Fluor 546 goat anti-mouse (1:1,000; Molecular Probes) and Alexa Fluor 488 goat anti-rabbit (1:400; Molecular Probes). Negative controls were obtained by omitting the primary antibodies; in all cases, no reactivity was observed. DAPI (4′,6-diamidino-2-phenylindole, 1 mg/mL, Sigma) was used to stain nuclei. Images were acquired using an Operetta Imaging System (Perkin Elmer Inc.).

Dezonne R.S., Sartore R.C., Nascimento J.M., Saia-Cereda V.M., Romão L.F., Alves-Leon S.V., de Souza J.M., Martins-de-Souza D., Rehen S.K, & Gomes F.C. (2017). Derivation of Functional Human Astrocytes from Cerebral Organoids. Scientific Reports, 7, 45091.

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Immunofluorescent Analysis of Neurogenesis in Coronal Brain Sections

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Coronal sections (thickness 60 μm) were prepared from the fixed brain tissues of the offspring using a vibratome (VT 1000S, Leica, Germany). Immunofluorescence analysis was carried out as described previously (Zhu et al., 2020 (link)). For immunofluorescent detection of BrdU, brain sections were denatured for 30 minutes in 2 M HCl at 37°C, rinsed 3 times (8 minutes per wash) in 0.1 M PB, followed by a 30-minute wash in 0.1 M borate buffer (pH 8.5), and then rinsed with 0.1 M PB before incubation with the primary detection antibodies.
The following primary detection antibodies were used in this study: rat anti-BrdU (1:500, Millipore, Temecula, CA); rabbit anti-Prox1 (1:500, Abcam, Cambridge, UK); rabbit anti-Tbr2 (1:500, Invitrogen); rabbit anti-BLBP (1:500, Millipore); mouse anti-NeuN (1:500, Millipore); rabbit anti-Reelin (1:500, Invitrogen); rabbit anti-parvalbumin (PV; 1:500, Invitrogen); rabbit anti-GAD (1:500, Invitrogen); and rabbit anti-Iba1 (1:500, Abcam). The secondary detection antibodies were as follows: Alexa Fluor 488 goat anti-mouse IgG (1:500, Abcam); Alexa Fluor 488 donkey anti-rabbit IgG (1:500, Invitrogen); Alexa Fluor 568 donkey anti-rabbit IgG (1:500, Cell Signaling Technology, Boston, MA); Alexa Fluor 647 goat anti-rat IgG (1:500, Chemicon, Nürnberg, Germany); and Alexa Fluor 647 donkey anti-rabbit IgG (1:500, Invitrogen).

Zhu X., Wu Y., Pan J., Li C., Huang J., Cui E., Chen Z., Zhou W., Chai X, & Zhao S. (2020). Neuroinflammation Induction and Alteration of Hippocampal Neurogenesis in Mice Following Developmental Exposure to Gossypol. International Journal of Neuropsychopharmacology, 24(5), 419-433.

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