Authentic standards of (E)-2-hexenal, p-xylene, α-pinene, β-pinene, trans-isolimonene, β-myrcene, 2-carene, α-phellandrene, 3-carene, α-terpinene, p-cymene, β-phellandrene, (Z)-β-ocimene, (E)-β-ocimene, γ-terpinene, terpinolene, n-nonanal, allo-ocimene, methyl salicylate, β-elemene, (Z)-jasmone, (E)-β-caryophyllene, and α-humulene (>95% purity) were purchased from Sigma-Aldrich (St. Louis, MO, United States). Dichloromethane (99.9% purity) was purchased from Merck (Germany).
2 carene
Manufactured by Merck Group
Sourced in United States
2-carene is a laboratory chemical compound used in research and development applications. It is a bicyclic monoterpene hydrocarbon that can be utilized in various analytical and experimental procedures. The core function of 2-carene is to serve as a reference standard or starting material for scientific investigations, however a detailed description of its intended use is not available while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
α pinene, by Merck Group (3 mentions)
Methyl salicylate, by Merck Group (2 mentions)
β myrcene, by Merck Group (2 mentions)
3 carene, by Merck Group (2 mentions)
β pinene, by Merck Group (2 mentions)
α phellandrene, by Merck Group (2 mentions)
γ terpinene, by Merck Group (2 mentions)
Linalool, by Merck Group (2 mentions)
β elemene, by Merck Group (1 mentions)
E 2 hexenal, by Merck Group (1 mentions)
α humulene, by Merck Group (1 mentions)
E β ocimene, by Merck Group (1 mentions)
Z β ocimene, by Merck Group (1 mentions)
Allo ocimene, by Merck Group (1 mentions)
E β caryophyllene, by Merck Group (1 mentions)
β phellandrene, by Merck Group (1 mentions)
P xylene, by Merck Group (1 mentions)
P cymene, by Merck Group (1 mentions)
α terpinene, by Merck Group (1 mentions)
Terpinolene, by Merck Group (1 mentions)
5 protocols using 2 carene
1
Authentic Standards for Volatile Compounds
2
Evaluating Antioxidant and Cytotoxic Potential
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The essential oil standards (α-pinene, β-pinene, 2-carene, 3-carene, limonene, linalool, and caryophyllene), BHT, PG, Vc, pyrogallol, thiobarbituric acid (TBA), trichloroacetic acid (TCA), dimethyl sulfoxide (DMSO), and 2,2-diphenyl-1-picrylhydrazyl (DPPH), etc. were purchased from Sigma and Sigma-Aldrich Company, United States. Other chemicals used were of analytical reagent grade. The fetal bovine serum (FBS) and Dulbecco’s modified eagle’s medium (DMEM) were obtained from Gibco Co., United States. Double-antibody (penicillin-streptomycin) was purchased from HyClone Co., United States. Methyl thiazolyl tetrazolium (MTT) was purchased from Solarbio Biotechnology Co., Beijing, China. 2′,7′-Dichlorofluorescein diacetate (DCFH-DA) assay kit was bought from Beyotime Institute of Biotechnology, Shanghai, China. Distilled water was used throughout.
3
Essential Oil Compounds Procurement
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Thymol methyl ether, Carvacrol methyl ether, (+)-2-carene, 1-octen-3-ol, myrcene, trans-anethole, α-terpineol were purchased from Sigma-Aldrich (St. Louis, MO, USA). Carvacrol, γ-terpinene, 3-carene, p-cymene, camphene, α-terpinene, terpinolene, (+/−)-α-pinene, (−/−)-β-pinene, R-(+)-limonene, S-(−)-limonene, were purchased from Extrasynthese (Genay, France). Thymol, linalool, α-phellandrene were purchased from Merck Millipore (Boston, MA, USA). All reagents and solvents have European Pharmacopoeia quality.
4
Terpene Biosynthesis Protocols
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Standards used include cis-3,7-dimethyl-2,6-octadien-1-ol (Aldrich, 268909-5ML), α-pinene (Aldrich, P-7408), β-myrcene (Sigma, M-0382), 1,8-cineole (Aldrich, C8,060-1), γ-terpinene (Aldrich, T2134), limonene (Sigma-Aldrich, 62118), camphene (Sigma-Aldrich, 456055), alpha-phellandrene (Sigma-Aldrich, W285611), 2-carene (Sigma-Aldrich, 232386), beta-phellandrene (Carbosynth, FB158830), linalool (Aldrich, L2602), and a 70% sabinene solution (kindly donated by VIORYL S.A., Athens, Greece). Phusion High-Fidelity DNA Polymerase (New England BioLabs, M0530S) and MyTaq DNA polymerase (BIO-21105, Bioline) were used in PCR amplifications. The QIAquick Gel Extraction Kit (#28704, Qiagen) was used for gel extraction and DNA purification. The NucleoSpin Plasmid Kit (740588.250, Macherey-Nagel) was used for plasmid DNA purification. Restriction enzymes used were from New England BioLabs.
5
Analytical Standards for Volatiles
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(≥97%), (R)-(+)-α-pinene (99%), (1S)-(-)-β-pinene (99%), 3-isopropyl-2-methoxypyrazine (≥97%), nonanal (95%), tridecane (˃95%), and methyl dihydrojasmonate (mixture of cis and trans) were purchased from Sigma Aldrich (St, Louis, MO, USA). Methyl salicylate (97% purity) was purchased from Sigma Aldrich (Steinhelm, Germany), (+)-(2)-carene (97% purity) from Sigma Aldrich (Switzerland) and (-)-trans caryophyllene (99%) from Fluka. For βphellandrene, we used Angelica seed oil containing 89% (S)-(+)-β-phellandrene (SigmaAldrich, Gillingham, Dorset, UK).
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