Elecsys hgh

Blood concentrations of lactate, catecholamines (including adrenaline, noradrenaline, and dopamine), cortisol, GH, IGF-1, and BDNF in the collected blood samples were analyzed. Biochemical analyses for all the indices except lactate were blindly conducted by SRL Inc. (Tokyo, Japan). Specifically, concentrations of catecholamines were measured using high-performance liquid chromatography (CA Test TOSOH; Tosoh Corporation, Tokyo, Japan), those of cortisol, GH, and IGF-1 were measured using electrochemiluminescence immunoassay (cortisol: Elecsys^® Cortisol; Roche Diagnostic Systems, Basel, Switzerland, GH: Elecsys^® hGH; Roche Diagnostic Systems, Basel, Switzerland, IGF-1: IGF-I IRMA DAIICHI; Fujirebio Inc., Tokyo, Japan), and the concentration of BDNF was measured using an enzyme-linked immunosorbent assay (Quantikine^TM ELISA Human Free BDNF Immunoassay; R&D Systems Inc., Minneapolis, MN, USA). As mentioned above, LA was measured at each blood collection using an enzyme electrode method (Lactate Pro™2 LT-1730; ARKRAY Inc., Kyoto, Japan).

Glucose was measured in the centralized accredited laboratories of the University of Palermo with the standard methods. HbA1c levels were determined by HPLC with an ion-exchange resin (BioRad D10, BioRad). Serum insulin was measured by electrochemiluminescence (ECLIA, Elecsys Insulin, Roche). The sensitivity of the method was 0.4 µU/mL. The normal range (µU/mL) was 2.6–24.9. Serum GH levels were measured by Immunoassay in electrochemiluminescence (ECLIA, Elecsys hGH, Roche). The lower limit of detection of the assay was 0.030 µg/L. The intra- and inter-assay coefficients of variation (CV) were 0.6–5.0 and 3.8–5.0%, respectively. We reported GH concentrations in µg/L of IS 98/574. Serum IGF-I levels were measured by means of a chemiluminescent immunometric assay (Immulite 2000; Diagnostic Products Corp., Los Angeles, CA, USA) using murine monoclonal anti-IGF-I antibodies. The standards were calibrated against the World Health Organization second IS 87/518. The sensitivity was 1.9 µg/L. The intra- and inter-assay CVs were 2.3–3.9% and 3.7–8.1%, respectively.

Plasma GDF15 was measured using a commercially available ELISA assay (R&D systems, human: cat no.: SGD150, and mouse: cat no.: DY6385). Intraplate CV was 2.8% and interplate CV was 5.6%. All samples were analyzed in duplicates. Plasma cortisol and growth hormone were measured using an immunological assays, Elecsys Cortisol II (Roche, Basal, Switzerland) and Elecsys hGH (Roche, Basal, Switzerland) automated on a cobas e801. The assay CV were 8% and 6% respectively.