Log In Sign up
The largest database of trusted experimental protocols

Tris 2 carboxyethyl phosphine tcep

Manufactured by GoldBio
Visit Supplier
Sourced in United States

Tris (2-carboxyethyl) phosphine (TCEP) is a reducing agent used in biochemical applications. It is a water-soluble phosphine compound that reduces disulfide bonds in proteins and peptides.

Automatically generated - may contain errors

Lab products found in correlation

Octyl glucoside, by GoldBio (2 mentions) Isopropyl β d 1 thiogalactopyranoside (iptg), by GoldBio (2 mentions) Adenosine triphosphate (atp), by Merck Group (2 mentions) Adocbl, by Merck Group (2 mentions) Dithiothreitol (dtt), by GoldBio (2 mentions) Adenosine monophosphate, by Merck Group (1 mentions) Adenosine diphosphate (adp), by Merck Group (1 mentions) Guanosine diphosphate, by Merck Group (1 mentions) Sodium triphosphate pentabasic, by Merck Group (1 mentions) Uridine monophosphate, by Merck Group (1 mentions) Guanosine triphosphate, by Merck Group (1 mentions) Guanosine monophosphate, by Merck Group (1 mentions) Coenzyme a, by Merck Group (1 mentions) Cocl2 6h2o, by Merck Group (1 mentions) Guanidine hcl, by Avantor (1 mentions) Pet his6 sumo tev lic cloning vector, by Addgene (1 mentions) Tris hcl, by Avantor (1 mentions) Glutamine gln, by Thermo Fisher Scientific (1 mentions) Sephadex g 25 resin, by Cytiva (1 mentions) Phusion site directed mutagenesis kit, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Tris(2-carboxyethyl)phosphine (3 citations) Tris (2‐carboxyethyl) phosphine hydrochloride (TCEP) (3 citations)

8 protocols using tris 2 carboxyethyl phosphine tcep

1

Biotin-PEG2-maleimide Ligand Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
All reagents were purchased from Fisher Scientific unless otherwise stated. Biotin-PEG2-maleimide ligand (PEG: polyethylene glycol) and anti-biotin antibody clone BTN.4 were purchased from ThermoFisher Scientific. Urea was purchased from Boston Bioproducts. Tris (2-carboxyethyl) phosphine (TCEP) and octyl-glucoside (OG) were purchased from Gold Biotechnology. Mutagenic oligonucleotides were purchased from Eurofins MWG Operon. 1,2-diphytanoyl-sn-glycerol-3-phosphocholine (DPhPC) lipid was purchased from Avanti Polar Lipids. StreptAvidin was purchased from Promega. Avidin was from Sigma-Aldrich and anti-biotin antibody SB58C clone from Southern Biotech.
Fahie M.A., Candido J., Andree G, & Chen M. (2021). Tuning protein discrimination through altering the sampling interface formed between the analyte and OmpG nanopore. ACS sensors, 6(3), 1286-1294.
+ Open protocol
+ Expand
2

Purification and Analysis of Enzymes

Check if the same lab product or an alternative is used in the 5 most similar protocols
adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), Coenzyme A (CoA), guanosine triphosphate (GTP), guanosine diphosphate (GDP), guanosine monophosphate (GTP), uridine monophosphate (UMP), β,γ-Methyleneguanosine 5′-triphosphate (GMPPCP), sodium triphosphate pentabasic (PPPi), and AdoCbl were from Sigma-Aldrich. Isopropyl β-D-1-thiogalactopyranoside (IPTG) and Tris (2-carboxyethyl) phosphine (TCEP) were from gold biotechnology. Ni(II)-NTA resin was from Qiagen.
Gouda H., Mascarenhas R., Ruetz M., Yaw M, & Banerjee R. (2023). Bivalent molecular mimicry by ADP protects metal redox state and promotes coenzyme B12 repair. Proceedings of the National Academy of Sciences of the United States of America, 120(11), e2220677120.
+ Open protocol
+ Expand
3

Synthesis and Purification of Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Co(Cl)2·6H2O, CH3I, and iPrBr were obtained from Sigma-Aldrich; Co(OAc)2·3H2O was obtained from Mallinckrodt; 4-methylpyridine (pyMe) and 4-vinylpyridine (pyv) were obtained from Oakwood Chemicals; and all deuterated solvents were purchased from Cambridge Isotope Laboratory. Dry solvents were obtained from a Jorg. C. Meyer solvent purification system. Ni-NTA resin was obtained from Cube BioTech and Thermo Fisher Scientific, while Sephadex G25 resin was obtained from Cytiva. Guanidine HCl and Tris HCl were purchased from VWR. Dithiothreitol (DTT) and tris(2-carboxyethyl)phosphine (TCEP) were purchased from Gold-Bio. Isopropylthio-β-D-galactoside (IPTG), 5,5-dithio-bis(2-nitrobenzoic acid) (DTNB), and glutamine (Gln) were purchased from Thermo Fisher Scientific. All chemicals were used as ordered without further purification. All buffers were prepared using 18.2 MΩ cm resistivity water from a Milli-Q water purification system. The pET His6 SUMO TEV LIC cloning vector was purchased from Addgene, and E. coli BL21 (DE3) cells were purchased from Novagen. TEV protease was expressed and purified according to literature protocols.
Neuwirth C., Madec S., Siebor E., Pechinot A., Duez J.M., Pruneaux M., Fouchereau-Peron M., Kazmierczak A, & Labia R. (2001). TEM-89 β-Lactamase Produced by a Proteus mirabilis Clinical Isolate: New Complex Mutant (CMT 3) with Mutations in both TEM-59 (IRT-17) and TEM-3. Antimicrobial Agents and Chemotherapy, 45(12), 3591-3594.
+ Open protocol
+ Expand
4

Protein Expression and Purification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Yeast extract, tryptone, NaCl, BisTris, Tris-base, glycine, β-mercaptoethanol (BME), agar, ammonium persulfate, sodium acetate, acetic acid, EDTA, and TEMED were purchased from Thermo Fisher Scientific (Hampton, NH, USA). DTT, isopropyl 1-thio--d-galactopyranoside, and tris(2-carboxyethyl)phosphine (TCEP) were purchased from GoldBio (St. Louis, MO, USA). Imidazole, Zn2SO4, and Cu(II)2SO4, Imidazole, monobasic and dibasic sodium phosphate, and acetonitrile were purchased from Sigma-Aldrich (St. Louis, MO, USA). Trichloroethanol was purchased from Acros Organics. Primers for mutagenesis were purchased from Sigma-Aldrich, and the Phusion site-directed mutagenesis kit was from Thermo Fisher Scientific. TPEN was purchased from Tocris Bioscience (Bristol, United Kingdom). Alexa-488 fluorescent dye for labeling was purchased from Life Technologies (Carlsbad, CA, USA). Bacterial strains used were DH5alpha (Invitrogen, St. Louis, MO, USA), BL21 pLysS (DE3) E. coli (Promega, Madison, WI, USA), and XL1-Blue (Stratagene, St. Louis, MO, USA). Chromatography columns were purchased from GE Healthcare (Marlborough, MA, USA).
Boyd S.D., Ullrich M.S., Calvo J.S., Behnia F., Meloni G, & Winkler D.D. (2020). Mutations in Superoxide Dismutase 1 (Sod1) Linked to Familial Amyotrophic Lateral Sclerosis Can Disrupt High-Affinity Zinc-Binding Promoted by the Copper Chaperone for Sod1 (Ccs). Molecules, 25(5), 1086.
+ Open protocol
+ Expand
5

Homocysteine Metabolism Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Amicon Ultra 15 centrifugal filters, D,L-homocysteine, L-serine, sodium phosphate, trifluoroacetic acid (TFA), tris(hydroxymethyl)aminomethane (Tris), and 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (HEPES) were all purchased from Sigma. Ammonium hydroxide, hydrochloric acid, and sodium dodecyl sulfate were purchased from Fisher. Ethylenediaminetetraacetic acid (EDTA), imidazole, and lead acetate were purchased from Acros Organics. [14C]-serine was from PerkinElmer. Tris (2-carboxyethyl) phosphine (TCEP) was from GoldBio. S-adenosyl-L-methionine (the disulfate tosylate salt) was purchased from BioVision. Trypsin was obtained from the Hampton Research Proti-ACE kit. EcoLite scintillation cocktail was obtained from MP Biomedicals. AG-50W-X8 anion exchange packing material was obtained from Bio-Rad. The Oligos for site directed mutagenesis were purchased from IDT. Luria Bertani (LB) broth was purchased from Research Products International (RPI).
Roman J.V., Mascarenhas R., Ceric K., Ballou D.P, & Banerjee R. (2023). Disease-causing cystathionine β-synthase linker mutations impair allosteric regulation. The Journal of Biological Chemistry, 299(12), 105449.
+ Open protocol
+ Expand
6

Purification and Characterization of Recombinant Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
AdoCbl, ATP, GTP, PPPi, and dithionitrobenzoic acid were from Sigma-Aldrich. The primers used for mutagenesis were purchased from Integrated DNA Technologies. IPTG and Tris (2-carboxyethyl) phosphine (TCEP) were from gold biotechnology. Nickel-nitrilotriacetic acid (Ni(II)-NTA) resin was from Qiagen.
Gouda H., Mascarenhas R., Pillay S., Ruetz M., Koutmos M, & Banerjee R. (2021). Patient mutations in human ATP:cob(I)alamin adenosyltransferase differentially affect its catalytic versus chaperone functions. The Journal of Biological Chemistry, 297(6), 101373.
+ Open protocol
+ Expand
7

Droxinostat Purification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The HDAC inhibitor 4-(4-chloro-2-methylphenoxy)-N-hydroxybutanamide (Droxinostat) was obtained from Sigma Aldrich and was used without further purification. Tris(2-carboxyethyl)phosphine (TCEP) was purchased from GoldBio. All other reagents were purchased from Fisher unless otherwise specified.
Leng K.R., Castañeda C.A., Decroos C., Islam B., Haider S.M., Christianson D.W, & Fierke C.A. (2019). Phosphorylation of Histone Deacetylase 8: Structural and Mechanistic Analysis of Phosphomimetic S39E Mutant. Biochemistry, 58(45), 4480-4493.
+ Open protocol
+ Expand
8

Membrane Protein Functionalization Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
All reagents and materials were purchased from Fisher Sci. or Boston Bioproducts unless otherwise noted. Mouse anti-biotin monoclonal antibody BTN.4 clone (MS-1048-P1) was purchased from Thermo Scientific. Avidin (A9275) was purchased from Sigma. Streptavidin (Z7041) was purchased from Promega. Maleimide-PEG45-biotin molecule was purchased from Creative PEG works (Biotin-PEG-MAL, MW 2k (PJK-1930)). Maleimide-PEG2-biotin and maleimide-PEG11-biotin were purchased from (ThermoFisher Scientific). Diphytanoylphosphatidylcholine (DPhPC) were from Avanti polar lipids. Tris(2-carboxyethyl)phosphine (TCEP) and octyl-glucoside (OG) and were purchased from GoldBio Technology.
Fahie M.A., Yang B., Pham B, & Chen M. (2016). Tuning the selectivity and sensitivity of an OmpG nanopore sensor by adjusting ligand tether length. ACS sensors, 1(5), 614-622.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!