Human carcinoma cells (HeLa, The Russian collection of human, animals and plants cell cultures, Institute of Cytology, St. Petersburg, Russia) were grown in Dulbecco’s modified Eagle’s medium (Paneco, Moscow, Russia) supplemented with 10% heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA), 2 mM L-glutamine (Paneco, Moscow, Russia) and antibiotic-antimycotic solution (Gibco, Waltham, MA, USA). To inhibit RNA transcription, the cells were grown in medium with 0.1 μg/ml actinomycin D (ActD). Cellular transfection was performed using the Lipofectamine 2000 reagent (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions.
Dulbecco s modified eagle s medium
Manufactured by PanEco
Dulbecco's modified Eagle's medium (DMEM) is a cell culture medium used to support the growth of various cell lines in vitro. It provides a balanced salt solution, amino acids, vitamins, and other nutrients required for cell proliferation and maintenance.
Automatically generated - may contain errors
Lab products found in correlation
L glutamine, by PanEco (2 mentions)
Hek293a cell line, by Thermo Fisher Scientific (2 mentions)
Turbofect, by Thermo Fisher Scientific (2 mentions)
Antibiotic antimycotic solution, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions)
Penicillin, by PanEco (1 mentions)
Streptomycin, by PanEco (1 mentions)
2 protocols using dulbecco s modified eagle s medium
1
HeLa Cell Culture with Actinomycin D
2
Efficient Transfection of HEK293A Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The 293A cell line is a subclone of the 293 cell line and has a relatively flat morphology suitable for screening adenoviral constructs. HEK293A cell line (Invitrogen) was cultured at 37 °C in a humidified atmosphere containing 5% CO 2 in Dulbecco's modified Eagle's medium (PanEco, Moscow, Russia) supplemented with 10% fetal bovine serum (HyClone, Logan, UT, USA), 1 × antibiotic mixture of penicillin and streptomycin (PanEco, Russia) and 2 mM L- glutamine (PanEco). Transfection of HEK293A cell line with genetic construct pAd-VEGF165-FuP2A-FGF2 was carried out using the transfection reagent TurboFect (Thermo Scientific, Rockford, IL, USA) according to the procedure recommended by the manufacturer. Conformation of recombinant protein expression was performed 48 h after transfection by immunofluorescent analysis.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!