Cells were fixed with 4% paraformaldehyde for 30 min and then labeled with 20 μL labeling buffer for 2 h at 37°C in a humidified box according to the TUNEL Apoptosis Detection Kit (Bosterbio, Pleasanton, CA, USA; #MK1023) protocol. After incubation with the blocking reagent from the kit for 30 min, the cells were incubated with anti‐DIG‐biotin for 30 min, incubated with SABC‐FITC and mounted with DAPI Fluoromount‐G (Southern Biotech, Birmingham, AL, USA; #0100‐20). The cells were then observed with an FSX100 microscope (Olympus, Tokyo, Japan).
Tunel apoptosis detection kit
Manufactured by Boster Bio
Sourced in United States
The TUNEL Apoptosis Detection Kit is a laboratory tool used to detect and analyze apoptosis, a form of programmed cell death, in biological samples. It provides a method for identifying DNA fragmentation, a hallmark of apoptosis.
Automatically generated - may contain errors
Lab products found in correlation
Dapi fluoromount g, by Southern Biotech (1 mentions)
Fsx100 microscope, by Olympus (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Solarbio (1 mentions)
Annexin 5 and pi, by Thermo Fisher Scientific (1 mentions)
Cell light apollo stain kit, by RiboBio (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
4 protocols using tunel apoptosis detection kit
1
TUNEL Assay for Apoptosis Detection
2
Apoptosis Detection in Tumor Tissues
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Paraffin-embedded tumor tissues were cut into 4-μm thick slices. Next, the TUNEL Apoptosis Detection Kit (FITC) purchased from BOSTER was used for detecting cell apoptosis in tumor tissues according to the manufacturer's instructions. The nuclei was counterstained with DAPI (Solarbio). Images were then observed under a fluorescence microscope (OLYMPUS).
3
Apoptosis Detection in SGC-7901 Cells
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After treatment with PBS, EVs-Vector, or EVs-L-PGDS, SGC-7901 cells were harvested and stained with Annexin V and PI (Invitrogen) according to the manufacturer's instruction. The apoptotic cells were detected by flow cytometry. The apoptotic cells in tumor tissue were evaluated by TUNEL Apoptosis Detection Kit (Boster, Wuhan, China) according to the manufacturer's instructions. The positive cells were visualized and photographed with a light microscope.
4
Zfp521-siRNA Transfection in IL-1β-Induced Chondrocytes
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Chondrocytes induced by IL-1β were transfected six hours after the DMEM/F-12 medium containing Lipofectamine 3000 (Invitrogen) plus Zfp521-siRNA oligos (RiboBio) was added. Then, DMEM/F-12 medium was replaced with complete media and incubated for up to 48 h. The sequence of the Zfp521-siRNA was 5’-GAACAGACATCGCTTAAGA-3’. The EdU assay was conducted using a Cell-Light Apollo Stain Kit after the siRNA transfection referring to the manufacturer’s protocol (RiboBio). The TUNEL assay was performed using a TUNEL Apoptosis Detection Kit according to the manufacturer’s protocol (Boster). The images were obtained by fluorescence microscopy, and the EdU-positive and TUNEL-positive cells were calculated using Image J.
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