Cells or tissues were collected/homogenized and lysed on ice in an RIPA buffer (25 mM Tris–HCl, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% NP-40 and 5% glycerol) with protease inhibitors (#88660SPCL, Thermo Fisher Scientific) and a phosphatase inhibitor mix (sc-45044, Santa Cruz). Immunoblotting was performed by probing with the following antibodies: anti-Nox4 (sc-30141), anti-Lamin (sc-376248) and anti-HuR (sc-5261) from Santa Cruz; monoclonal anti-GAPDH (G8795) from Sigma; and anti-fibronectin (ab2413) and anti-SMA (ab5694) from Abcam.
Anti hur sc 5261
Manufactured by Santa Cruz Biotechnology
Sourced in Germany
Anti-HuR (sc-5261) is a primary antibody that specifically recognizes the HuR protein. HuR is an RNA-binding protein that plays a role in the regulation of gene expression. This antibody can be used for the detection of HuR in various experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti fibronectin ab2413, by Abcam (1 mentions)
Sc 45044, by Santa Cruz Biotechnology (1 mentions)
Anti gapdh g8795, by Merck Group (1 mentions)
Anti gm130 11308 1 ap, by Proteintech (1 mentions)
Ab18529, by Abcam (1 mentions)
Ab92726, by Abcam (1 mentions)
Anti gapdh, by BBI Lifesciences (1 mentions)
D110016 0100, by BBI Lifesciences (1 mentions)
G 6983, by Merck Group (1 mentions)
U0126, by Merck Group (1 mentions)
Ku55933, by Merck Group (1 mentions)
Ecl system, by GE Healthcare (1 mentions)
Z vad fmk, by R&D Systems (1 mentions)
Anti caspase 2, by BD (1 mentions)
Goat anti rabbit sc 2054, by Santa Cruz Biotechnology (1 mentions)
Radionucleotides, by PerkinElmer (1 mentions)
Anti lamin b1 sc 20682, by Santa Cruz Biotechnology (1 mentions)
Anti β actin, by Merck Group (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Sb203580, by Merck Group (1 mentions)
3 protocols using anti hur sc 5261
1
Immunoblotting Analysis of Cellular Proteins
2
Western Blot Analysis of Exosomal Proteins
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Total protein of indicated cells or isolated exosomes was extracted using RIPA Lysis Buffer (Beijing, China) at 4°C for 30 min. Protein concentration was determined by PierceTM BCA Protein Assay Kit. Proteins were then concentrated on SDS-PAGE (6%) and separated by SDS-PAGE (12%), and transferred to nitrocellulose filter membranes. After blocking with 3% BSA, membranes were subsequently incubated with primary antibody anti-HuR (sc5261, Santa Cruz), anti-GM130 (11308-1-AP, Proteintech), anti-TSG101 (ab83, Abcam), anti-CD9 (ab92726, Abcam), anti-Lamp2b (ab18529, Abcam) and anti-GAPDH (D110016-0100, BBI Life Sciences). After washing three times in TBST, the membranes were incubated with horseradish peroxidase-conjugated secondary antibody at room temperature for 1 h.
3
Apoptotic Signaling Pathways in Cell Culture
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All cell culture media, supplements and modifying enzymes were purchased from Invitrogen (Karlsruhe, Germany) if not otherwise indicated. Doxorubicin was from Biotrend Chemicals (Cologne, Germany), paclitaxel, and propidium iodide were obtained from Sigma-Aldrich (Taufkirchen, Germany). Gö6983, KU55933, SB203580, U0126 and Chk2 inhibitor II were from Merck (Darmstadt, Germany). The caspase inhibitors Z-VDVAD-FMK and Z-VAD-FMK were derived from R & D Systems (Wiesbaden, Germany). The following antibodies were used in this study: anti-HuR (sc-5261) and anti-Lamin B1 (sc-20682) from Santa Cruz (Heidelberg, Germany), anti-caspase-2 (#611022, BD Biosciences, Heidelberg, Germany), anti-caspase-3 (#9662), anti-caspase-7 (#9494) anti-PARP (#9542) and anti-Bid (#2002) antibodies were from Cell Signaling (Frankfurt, Germany), and anti-β-actin (#A2228) from Sigma-Aldrich. The goat anti-rabbit (sc-2054) and goat anti-mouse (sc-20559) HRP-linked antibodies were from Santa Cruz and Alexa Fluor 488 goat anti-mouse was from Life Technologies (Darmstadt, Germany). Radionucleotides were from Perkin Elmer (Rodgau, Germany) and the ECL system and Hyperfilms were from GE Healthcare (Freiburg, Germany).
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