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Calibration solutions

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Calibration solutions are reference materials used to verify and validate the accuracy and precision of analytical instruments and equipment in laboratory settings. These solutions are designed to provide a known concentration of specific analytes, allowing for the calibration and performance assessment of a wide range of laboratory instruments, such as pH meters, spectrophotometers, and ion-selective electrodes.

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Lab products found in correlation

Q exactive, by Thermo Fisher Scientific (1 mentions) Ultimate 3000 rs, by Thermo Fisher Scientific (1 mentions) Kinetex hilic, by Phenomenex (1 mentions) Formic acid, by Thermo Fisher Scientific (1 mentions) Methanol, by Thermo Fisher Scientific (1 mentions) Acetonitrile, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Pierce™ LTQ ESI Positive Ion Calibration Solution (9 citations) Pierce Positive and Negative Ion Calibration Solutions (7 citations) Positive and negative ion calibration solutions (5 citations) Pierce Negative Ion Calibration Solution (4 citations) Premixed calibration solutions (3 citations) Negative ion calibration solution (3 citations) Pierce LTQ ESI Positive Calibrations solution (2 citations) Pierce LTQ ESI Negative Calibrations solution (2 citations) PierceTM FlexMix Ion Calibration Solutions (2 citations) Pierce™ FlexMix™ Calibration solution (2 citations)

3 protocols using calibration solutions

1

High-Resolution LC-MS Analysis of Ascarosides

Cited 1 time
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High-resolution LC-MS analysis was performed on a Dionex 3000 UPLC system coupled with a Thermo Q Exactive high-resolution mass spectrometer as described previously10 (link),46 (link). Metabolite extracts were separated using a water-acetonitrile gradient using Agilent ZORBOX Eclispse XDB-C18 rapid resolution column (2.1 × 150 mm, particle size 1.8-micron) maintained at 40 °C. Solvent A: 0.1% formic acid in water; Solvent B: 0.1% formic acid in acetonitrile. A/B gradient started at 5% B for 1.5 min after injection and increased linearly to 100% B at 12.5 min, using a flow rate of 0.5 mL/min. Mass spectrometer parameters: spray voltage 2.9 kV; capillary temperature 320 °C; prober heater temperature 300 °C; sheath, auxiliary, and spare gas 70, 2, and 0, respectively; S-lens RF level S5, resolution 140,000 at m/z 200, AGC target 1 × 106. The instrument was calibrated in negative and positive modes with m/z range from 200 to 1000 using calibration solutions (Thermo-Fisher). Ascarosides were detected in negative ionization mode as [M-H]- and MS/MS spectra and retention times confirmed by comparison with known standards.
Manohar M., Tenjo-Castano F., Chen S., Zhang Y.K., Kumari A., Williamson V.M., Wang X., Klessig D.F, & Schroeder F.C. (2020). Plant metabolism of nematode pheromones mediates plant-nematode interactions. Nature Communications, 11, 208.
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2

UHPLC-MS for Lipid Profiling

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Samples and standards were stored at − 20 °C until they could be analyzed. A Dionex UltiMate 3000RS ultra high performance liquid chromatography (UHPLC) (Sunnyvale, CA) system was used in conjunction with a Kinetex HILIC column (100 Å 2.6 μm 150 mm × 2.1 mm, Phenomenex, Torrance, CA) to separate lipid compounds for both the targeted and untargeted analysis. A 10 μL injection of sample was injected using a Dionex UltiMate 3000 RS autosampler kept at 4 °C. The syringe and fluidics were washed twice with 150 μL of 50:50 (v/v) water:2-propanol. All chromatographic conditions were controlled by Chromeleon Xpress version 6.80 and all mass spectrometric conditions were controlled by Xcalibur version 4.0. Mass calibration was performed every 24 h in positive and negative mode using calibration solutions from Thermo Scientific (Waltham, MA).
Tague E.D., Woodall B.M., Harp J.R., Farmer A.T., Fozo E.M, & Campagna S.R. (2019). Expanding lipidomics coverage: effective ultra performance liquid chromatography-high resolution mass spectrometer methods for detection and quantitation of cardiolipin, phosphatidylglycerol, and lysyl-phosphatidylglycerol. Metabolomics : Official journal of the Metabolomic Society, 15(4), 53.
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3

Identification of Tryptophan Metabolites

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Ultrapure grade water was purchased from EMD Millipore, and analytical grade formic acid, methanol, acetonitrile, and dimethylsulfoxid were from Fisher Scientific. Calibration solutions were from Thermo Scientific. Deuterated standards were purchased from Cambridge Isotope Laboratories (CIL), Buchem, or Acros Organics as follows: 3-hydroxy-DL-kynurenine (5,8,8-D3, 98% purity, Buchem) and 3-hydroxyanthranilic acid (4,6-D2, 98% purity, Buchem), anthranilic acid (3,4,5,6-D4, 98% purity, Buchem), Dl-glutamic acid (2,4,4-D3, 98% purity, CIL), fumaric acid (2,3-D2, 98% purity, CIL), kynurenic acid (3,4,6,7,8-D5, 98% purity, Buchem), kynurenine (D4, 98% purity, Buchem), L-tryptophan (indole-D5, 98% purity, CIL) nicotinic acid (D4, 98% purity, CIL), picolinic acid (99% purity, Acros), and quinolinic acid (4,5,6-D3, 99% purity, Buchem).
Blazeck J., Karamitros C.S., Ford K., Somody C., Qerqez A., Murray K., Burkholder N.T., Marshall N., Sivakumar A., Lu W.C., Tan B., Lamb C., Tanno Y., Siddiqui M.Y., Ashoura N., Coma S., Zhang X.M., McGovern K., Kumada Y., Zhang Y.J., Manfredi M., Johnson K.A., D’Arcy S., Stone E, & Georgiou G. (2022). Bypassing evolutionary dead ends and switching the rate-limiting step of a human immunotherapeutic enzyme. Nature catalysis, 5(10), 952-967.
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