Integrin expression profiling was performed with 4×104 MB-004 cells per reaction. Cells were pelleted and resuspended in 50 μL of a 1:100 dilution of anti-αVβ3 (Abcam), 1:100 dilution of anti-αVβ5 (Millipore), 1:25 dilution of anti-α5-FITC, or 1:25 dilution of anti-β1-AF488 (BioLegend) antibodies. Cells were incubated with primary antibodies for 40 min on ice and were then washed in PBS containing 0.1% bovine serum albumin (PBS/BSA). Cells treated with unlabeled antibodies were then incubated with goat anti-mouse IgG-FITC (Sigma) for 40 min and washed in PBS/BSA. Cells were analyzed on a Guava EasyCyte flow cytometer (EMD Millipore), and the resulting data was analyzed using FlowJo software (TreeStar).
Anti α5 fitc
Manufactured by BioLegend
Anti-α5-FITC is a fluorescently labeled antibody that binds to the α5 subunit of the integrin receptor. It is used in flow cytometry and other applications to detect and quantify the presence of the α5 integrin on cell surfaces.
Automatically generated - may contain errors
2 protocols using anti α5 fitc
1
Integrin Expression Profiling in MB-004 Cells
2
Integrin Expression Profiling in MB-004 Cells
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Integrin expression profiling was performed with 4×104 MB-004 cells per reaction. Cells were pelleted and resuspended in 50 μL of a 1:100 dilution of anti-αVβ3 (Abcam), 1:100 dilution of anti-αVβ5 (Millipore), 1:25 dilution of anti-α5-FITC, or 1:25 dilution of anti-β1-AF488 (BioLegend) antibodies. Cells were incubated with primary antibodies for 40 min on ice and were then washed in PBS containing 0.1% bovine serum albumin (PBS/BSA). Cells treated with unlabeled antibodies were then incubated with goat anti-mouse IgG-FITC (Sigma) for 40 min and washed in PBS/BSA. Cells were analyzed on a Guava EasyCyte flow cytometer (EMD Millipore), and the resulting data was analyzed using FlowJo software (TreeStar).
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