Solid stocks were purchased from the indicated suppliers and prepared as concentrated stock solutions in the appropriate solvent: hydroxyurea (100 mM in dH2O) from Acros, 2-deoxygluosce (1 M in dH2O), oxamate (100 mM in dH2O), 6-aminonicotinamide (2.5 mM in DMSO), piperlongumine (20 mM in DMSO), simvastin (20 mM in DMSO), L-buthionine-sulfoxamine (50 mM in dH2O) or chloroquine (20 mM in dH2O) from Sigma; GSK 2837808A (10 mM in DMSO) and metformin (100 mM in DMEM) from TOCRIS bioscience and TH588 (10 mM in DMSO) from Selleckchem. V158411 and VER-246008 were from Vernalis Research and prepared as 20 mM DMSO stocks. Compounds were serially diluted in the appropriate solvent to 500× or 1000× final concentration then to 5× or 10× final concentration in complete media before addition to cells to yield a 1× final concentration.
Th588
Manufactured by Selleck Chemicals
Sourced in United States
The TH588 is a laboratory instrument designed for chemical analysis. It features precise temperature control and monitoring capabilities.
Automatically generated - may contain errors
Lab products found in correlation
Chloroquine, by Merck Group (1 mentions)
Hydroxyurea, by Thermo Fisher Scientific (1 mentions)
Metformin, by Bio-Techne (1 mentions)
Oxamate, by Merck Group (1 mentions)
6 aminonicotinamide, by Merck Group (1 mentions)
Gsk2837808a, by Merck Group (1 mentions)
Piperlongumine, by Merck Group (1 mentions)
Multiskan spectrum spectrophotometer, by Thermo Fisher Scientific (1 mentions)
2 protocols using th588
1
Preparation of Compound Stock Solutions
2
Cell Viability Assay with TH588
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Cell viability was assessed using a Cell Counting Kit-8 assay (Engreen Biosystem Ltd., Beijing, China) according to the manufacturer's instructions. Briefly, cells (5,000 cells/well) were plated in 96-well plates and were allowed to attach for 8 h at 37°C prior to treatment with 2, 4, 6, 8, 10, 16 and 20 µM TH588 (Selleck Chemicals, Houston, TX, USA). The viability was determined following 72 h of treatment at 37°C by measuring the absorbance at 450 nm using a Multiskan Spectrum spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA).
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