The cells were fixed with 3.7% formaldehyde for 10 minutes and then permeabilized and blocked with 5% FBS in PBST (0.1% Tween 20 in PBS) for 1 hour at room temperature. The cells were then stained with primary antibodies (anti-albumin, Abcam; anti-EpCAM, Abcam; anti-OC2, LSBio, Seattle, WA, USA; anti-cytokeratin 18, Sigma-Aldrich) for overnight, followed by staining with secondary antibodies for 2 hours at room temperature. DAPI (Sigma-Aldrich) was used for staining of cell nuclei. Image J was used for quantification.
Anti cytokeratin 18
Manufactured by Merck Group
Sourced in United States, United Kingdom
Anti-cytokeratin 18 is a laboratory-grade antibody used to detect the presence of cytokeratin 18, a protein found in epithelial cells. This antibody can be used in various immunoassay techniques, such as immunohistochemistry and Western blotting, to identify and quantify cytokeratin 18 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using anti cytokeratin 18
1
Immunofluorescence Profiling of Liver Cells
2
Characterization of Primary Fibroblasts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Primary cultured fibroblasts were examined by immunofluorescence using anti-cytokeratin-18 (Sigma), human specific anti-vimentin (V9; Novocastra laboratories, Ltd., UK), anti-fibroblast surface protein (1B10; Sigma), anti-human CD31 (Santa Cruz, California), anti-α–SMA (1A4; Dako, Denmark), anti-PDGFRα (Dako), anti-human CD68 (1/100, BD Pharmingen™), anti-human CD163 (1/100, Bio-Rad), and anti-human F4/80 (1/50, Invitrogen) antibodies. The antibodies anti-AM, anti-CLR, anti-RAMP2, and anti-RAMP3 were developed and characterized in the laboratory.
3
Antibody Panel for Cell Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Antibodies against mouse antigens were purchased from BD Pharmingen unless otherwise specified, and included CD24-PE, CD49f-PE-cy5, CD29-PE-cy5 (Biolegend, San Diego, CA), CD29-FITC (Serotec, Oxford, UK). Rat anti-CD49f and purified rat anti-CD29 antibodies were purchased from BD Pharmingen. We also used anti-keratin 14 (covance, Princeton, NJ), anti-cytokeratin 18 (sigma, St. Louis, MO) and polyclonal rabbit anticytokeratin (Dako, Santa Clara, CA) antibodies.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!