For each printing run, three individual SOFs were dissolved in 100 mL of distilled water. Samples of the solutions were then diluted, and the drug concentration was determined through ultra-high performance liquid chromatography (UHPLC) using a UHPLC-DAD system. It consisted of a Thermo Scientific™ Dionex™ UltiMate™ 3000 BioRS equipped with a WPS-3000TBRS autosampler, and a TCC-3000RS column compartment set at 35 °C (Thermofisher Scientific, Waltham, MA, USA). The system was operated using Chromeleon 7 software. An Accucore C18 column (2.6 µm, 100 × 2.1 mm) combined with a security guard ultra-cartridge (Phenomenex Inc., Torrance, CA, USA) was used. An isocratic binary solvent system was utilized, consisting of water/formic acid (0.1%, v/v) as solvent A, and acetonitrile/formic acid (0.1%, v/v) as solvent B (90%A, 10%B). The flow rate of the mobile phase was 1.5 mL/minute, and the injection volume was 50 μL. Quantitative analysis of paracetamol in the SOFs was carried out using an external standard method. The calibration curve was constructed using 5 different standard levels in the concentration range 1–20 mg/L. The peak of paracetamol was monitored at 244 nm.
Dionex ultimate 3000 biors
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Dionex™ UltiMate™ 3000 BioRS is a high-performance liquid chromatography (HPLC) system designed for biopharmaceutical analysis. It features a modular design, precise flow control, and advanced detection capabilities to support a wide range of analytical applications.
Automatically generated - may contain errors
Lab products found in correlation
Accucore c18 column, by Phenomenex (3 mentions)
Security guard ultra cartridge, by Phenomenex (3 mentions)
Tcc 3000rs column, by Thermo Fisher Scientific (2 mentions)
Wps 3000tbrs, by Thermo Fisher Scientific (2 mentions)
Chromeleon 7, by Thermo Fisher Scientific (1 mentions)
Tcc 3000rs, by Thermo Fisher Scientific (1 mentions)
Strata x c18 column, by Phenomenex (1 mentions)
5 protocols using dionex ultimate 3000 biors
1
Paracetamol Quantification in Solid Oral Formulations
2
Paracetamol Quantification in SODF
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For each formulation, three individual SODFs were dissolved in 100 mL of distilled water. Samples of the solutions were then diluted and the drug concentration was determined by ultra-high performance liquid chromatography (UHPLC, Thermofisher Scientific, Waltham, MA, USA) using a UHPLC-DAD system. It consisted of a Thermo Scientific™ Dionex™ UltiMate™ 3000 BioRS equipped with a WPS-3000TBRS autosampler and a TCC-3000RS column compartment set at 35 °C. The system was operated using Chromeleon 7 software (Thermofisher Scientific, Waltham, MA, USA). An Accucore C18 column (2.6 µm, 100 × 2.1 mm2) combined with a security guard ultra-cartridge (Phenomenex Inc., Torrance, CA, USA) was used. An isocratic binary solvent system was utilized, consisting of water/formic acid (1%, v/v) as solvent A and acetonitrile/formic acid (1%, v/v) as solvent B (90%A, 10%B). The flow rate of the mobile phase was 1.5 mL/minute, and the injection volume was 50 μL. Quantitative analysis of paracetamol in the SODFs was carried out using an external standard method. The calibration curve was constructed using 5 different standard levels in the concentration range 1–20 mg/L. The peak of paracetamol was monitored at 244 nm.
3
Fractionation and Desalting of iTRAQ-Labeled Peptides
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The iTRAQ labeled peptides were mixed in equal amounts and fractioned by an HPLC system (Thermo Scientific Dionex UltiMate 3000 BioRS, Thermo Scientific, Shanghai, China) using the Durashell C18 column (5 μm, 100 Å, 4.6 mm × 250 mm). The dried peptides were dissolved in 100 μL buffer A (20 mM NH4HCO3 in 3% acetonitrile, pH 10), and were then loaded onto the HPLC device at a flow rate of 1 mL/min with buffer B (20 mM NH4HCO3, pH 10) at gradients of 0–8% for 5 min; 8–45% for 25 min; 45–80% for 15 min; and 80–100% for 5 min. The eluted fractions were collected at 1-min intervals and pooled into 12 fractions, then desalted with a Strata X C18 column (Phenomenex, Guangzhou, China) and vacuum-dried.
4
Quantification of Ibuprofen Formulations by UHPLC
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For each formulation, three SOFs were dissolved in 100 mL of a mixture (40%/60%) of solvent A (distilled water/formic acid (1%, v/v)) and solvent B (acetonitrile). Samples of the solutions were then diluted and the concentration of the drug was determined by ultra-high performance liquid chromatography (UHPLC) using a UHPLC-DAD system. This consisted of a Thermo Scientific™ Dionex™ UltiMate™ 3000 BioRS equipped with a WPS-3000TBRS autosampler and a TCC-3000RS column compartment set at 35 °C (Thermofisher Scientific, Waltham MA, USA). The system was operated using Chromeleon 7 software. An Accucore C18 column (2.6 µm, 100 mm × 2.1 mm) combined with a security guard ultra-cartridge (Phenomenex Inc., Torrance CA, USA) was used. An isocratic binary solvent system was utilized, consisting of solvent A and solvent B (40%A, 60%B). The flow rate of the mobile phase was 1.5 mL/minute, and the injection volume was 50 μL. Quantitative analysis of IbuAc and IbuNa in the SOFs was carried out using an external standard method. The calibration curve for each form of the drug was constructed using 5 different standard levels of the corresponding form in the concentration range 1–20 mg/L. The peak of ibuprofen was monitored at 258 nm.
5
Isobaric Peptide Labeling and Fractionation
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Samples were labeled with iTRAQ 8-plex Multiplex Reagent Kit (AB Sciex U.K.) by following the manufacturer's instructions. All labeled samples were mixed in equal volume and fractionated using a high-performance liquid chromatography (HPLC) system (Thermo Dionex Ultimate 3000 BioRS).
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