Analyzer 3.1 luminex 200 machine

Manufactured by Merck Group

Sourced in United States

The Analyzer 3.1 Luminex 200 machine is a multiplex assay platform that utilizes fluorescent-coded magnetic microspheres to detect and quantify multiple analytes simultaneously in a single sample. The core function of this instrument is to perform highly multiplexed bioassays, enabling the measurement of a wide range of biomolecules such as proteins, peptides, nucleic acids, and other targets.

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Lab products found in correlation

Milliplex mouse cytokine immunoassay kit, by Merck Group (2 mentions) Map mouse cytokine chemokine magnetic bead panel, by Merck Group (1 mentions)

3 protocols using analyzer 3.1 luminex 200 machine

Multiplex Cytokine Quantification

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Protein levels of IL-1β, IL-6, IL-10, MCP-1, MIP-2, and TNF-α in the serum were measured using a customized Milliplex Mouse Cytokine Immunoassay Kit (Millipore, 2620525) with Analyzer 3.1 Luminex 200 machine (Millipore, USA). Data were analyzed using corresponding software according to the manufacturer’s instructions.

Liu Y., Chu J.M., Yan T., Zhang Y., Chen Y., Chang R.C, & Wong G.T. (2020). Short-term resistance exercise inhibits neuroinflammation and attenuates neuropathological changes in 3xTg Alzheimer’s disease mice. Journal of Neuroinflammation, 17, 4.

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Cytokine Profile in Hippocampus

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The protein levels of pro- and anti-inflammatory cytokine including IL-1β, IL-10, MCP-1, and TNF-α in the hippocampus were measured using a customized Milliplex Mouse Cytokine Immunoassay Kit (Millipore, 2620525) with Analyzer 3.1 Luminex 200 machine (Millipore, USA). Data were analyzed using corresponding software according to the manufacturer’s instructions.

Liu Y., Chu J.M., Ran Y., Zhang Y., Chang R.C, & Wong G.T. (2022). Prehabilitative resistance exercise reduces neuroinflammation and improves mitochondrial health in aged mice with perioperative neurocognitive disorders. Journal of Neuroinflammation, 19, 150.

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Multiplex Cytokine Analysis of Murine Samples

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Protein levels of IL-1β, IL-6, MIP-2, TNF-α, and IL-10 in whole protein lysates or plasma were measured using a customized Milliplex Mouse Cytokine Immunoassay Kit (2620525)/MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel, MCYTOMAG-70K with Analyzer 3.1 Luminex 200 machine (Millipore, USA). Data were analyzed on corresponding software according to the manufacturer’s instructions.

Huang C., Irwin M.G., Wong G.T, & Chang R.C. (2018). Evidence of the impact of systemic inflammation on neuroinflammation from a non-bacterial endotoxin animal model. Journal of Neuroinflammation, 15, 147.

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