Acetonitrile (CH3CN) and Kryptofix2.2.2 (K222) were obtained from Merck (Darmstadt, Germany), and dry dimethyl sulfoxide (DMSO) was purchased from Sigma Aldrich (St. Louis, MO, USA). Sep-Pak light, Accell Plus QMA and Alumina N cartridges were from Waters, Milford, MA, USA. Phenomenex Luna pre-column (C18/2, 50 × 10 mm; 5 µm), Phenomenex Nucleosil columns (C18, 250 × 10 mm; 5 µm and C18, 250 × 4.6 mm) and 0.22 µm Millex GS and LX filters were from Millipore, Billerica, MA, USA. NCA [18F]fluoride was obtained from a PETtrace 16.5 MeV cyclotron incorporating a high-pressure niobium target (Cyclotek(AUST) Pty. Ltd., Victoria, Australia)) via the 18O(p,n)18F nuclear reaction. F-18 separation cartridges (Waters Accell Plus QMA Sep-Pak light, Kent, UK) were pre-conditioned with 0.5 M K2CO3 and subsequently rinsed with water. Radio-HPLC analyses were performed using a Shimadzu HPLC (SCL-10AVP system controller, SIL-10ADVP auto injector, LC-10ATVP solvent delivery unit, CV-10AL control valve, DGU-14A degasser, and SPD-10AVPV detector) Q6 coupled to a scintillation detector (Ortec 276 Photomultiplier Base with Preamplifier, Ortec 925-SCINT ACE mate Preamplifier, Amplifier, BIAS supply and SCA, and a Bicron 1M 11/2 Photomultiplier Tube).
Sep pak light
Manufactured by Waters Corporation
Sourced in United States
Sep-Pak light is a disposable cartridge used for sample preparation in analytical chemistry. It is designed to simplify the extraction and purification of analytes from complex sample matrices prior to analysis.
Automatically generated - may contain errors
Lab products found in correlation
Rp 18e, by Merck Group (2 mentions)
Scl 10avp, by Shimadzu (1 mentions)
Acetonitrile ch3cn, by Merck Group (1 mentions)
Dry dimethylsulfoxide dmso, by Merck Group (1 mentions)
Kryptofix2.2.2 k222, by Merck Group (1 mentions)
Trifluoroacetic acid, by Tokyo Chemical Industry (1 mentions)
N chlorosuccinimide, by Tokyo Chemical Industry (1 mentions)
Mediterranea sea18 column, by Teknokroma (1 mentions)
Ethanol, by Merck Group (1 mentions)
5 protocols using sep pak light
1
Radiolabeling of F-18 Compounds
2
Radiosynthesis of [18F]11 Compound
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Hands-on reaction: analytical
RCY, 27%
± 6% (n = 3). Automated synthesis: isolated
RCY, 10% ± 2% (n = 7). [18F]11 was obtained with a radiochemical purity of 99% and SA
of 6 GBq/μmol after sterile filtration. Precartridge purification
with a C18 cartridge (Waters Sep-Pak light): the product [18F]11 was released with 1 mL of 20% ethanol in water,
and the resulting fraction was further diluted with 1 mL of water.
HPLC purification was performed on a Chromolith performance column,
RP18-e (100 mm × 10 mm, Merck) using water and methanol containing
0.1% of formic acid as mobile phase (water/methanol 95:5 v/v, flow
rate 5 mL/min, λ = 254 nm). Retention time: 10 min.
Radiochemical
purity and SA of [18F]11 were determined by
analytical HPLC, using a Chromolith performance column, RP18-e (100
mm × 4.6 mm, Merck), and water and methanol containing 0.1% of
formic acid as mobile phase (methanol content from 5% to 20% over
5 min, at 20% for 2 min, and up to 70% over 2 min, flow 3 mL/min,
λ = 265 nm). Retention time: 4.0 min.
RCY, 27%
± 6% (n = 3). Automated synthesis: isolated
RCY, 10% ± 2% (n = 7). [18F]
of 6 GBq/μmol after sterile filtration. Precartridge purification
with a C18 cartridge (Waters Sep-Pak light): the product [18F]
and the resulting fraction was further diluted with 1 mL of water.
HPLC purification was performed on a Chromolith performance column,
RP18-e (100 mm × 10 mm, Merck) using water and methanol containing
0.1% of formic acid as mobile phase (water/methanol 95:5 v/v, flow
rate 5 mL/min, λ = 254 nm). Retention time: 10 min.
Radiochemical
purity and SA of [18F]
analytical HPLC, using a Chromolith performance column, RP18-e (100
mm × 4.6 mm, Merck), and water and methanol containing 0.1% of
formic acid as mobile phase (methanol content from 5% to 20% over
5 min, at 20% for 2 min, and up to 70% over 2 min, flow 3 mL/min,
λ = 265 nm). Retention time: 4.0 min.
3
Automated Synthesis of [18F]13 Tracer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Hands-on reaction: analytical
RCY 19%
± 6% (n = 3). Automated synthesis: isolated
RCY, 6% ± 1.5% (n = 3). [18F]13 was obtained with a radiochemical purity of 99% and SA
of 2 GBq/μmol after sterile filtration. Precartridge purification
with a C18 cartridge (Waters Sep-Pak light): the product [18F]13 was then released with 0.7 mL of pure ethanol,
and the resulting fraction was further diluted with 1.3 mL of water.
HPLC purification was performed on a Chromolith performance column,
RP18-e (100 mm × 10 mm, Merck), using water and methanol containing
0.1% of formic acid as mobile phase (water/methanol 70:30 v/v, flow
rate 5 mL/min, λ = 254 nm). Retention time: 7.1 min.
Radiochemical
purity and SA of [18F]13 were determined by
analytical HPLC, using a Chromolith performance column, RP18-e (100
mm × 4.6 mm, Merck), and water and methanol containing 0.1% of
formic acid as mobile phase (methanol content from 20% to 36% over
6 min, and up to 60% over 3 min, flow 3 mL/min, λ = 265 nm).
Retention time: 5.2 min.
RCY 19%
± 6% (n = 3). Automated synthesis: isolated
RCY, 6% ± 1.5% (n = 3). [18F]
of 2 GBq/μmol after sterile filtration. Precartridge purification
with a C18 cartridge (Waters Sep-Pak light): the product [18F]
and the resulting fraction was further diluted with 1.3 mL of water.
HPLC purification was performed on a Chromolith performance column,
RP18-e (100 mm × 10 mm, Merck), using water and methanol containing
0.1% of formic acid as mobile phase (water/methanol 70:30 v/v, flow
rate 5 mL/min, λ = 254 nm). Retention time: 7.1 min.
Radiochemical
purity and SA of [18F]
analytical HPLC, using a Chromolith performance column, RP18-e (100
mm × 4.6 mm, Merck), and water and methanol containing 0.1% of
formic acid as mobile phase (methanol content from 20% to 36% over
6 min, and up to 60% over 3 min, flow 3 mL/min, λ = 265 nm).
Retention time: 5.2 min.
4
Radiosynthesis of 211At-MABG for Cancer Therapy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The radionuclide 211At was produced by irradiation of a Bi target (New Metals and Chemicals, Essex, UK) with an external vertical beam and an NIRS AVF-930 cyclotron (Sumitomo Heavy Industries, Tokyo, Japan) with recovery through dry distillation, as described previously [20] (link). The 211At-MABG was radiosynthesized under no-carrier-added conditions through radiohalogenation of meta-trimethylsilylbenzylguanidine (ABX Advanced Biochemical Compounds, Radeberg, Germany) with N-chlorosuccinimide (Tokyo Chemical Industries, Tokyo, Japan) in trifluoroacetic acid (Tokyo Chemical Industries, Tokyo, Japan), as described previously [11] (link). Unpurified 211At-MABG was trapped on a tC18 cartridge (Sep-Pak light; Waters, Milford, MA), washed with 1 ml of pure water (Fujifilm Wako Pure Chemical), and then eluted as the final product with 2 ml of 5% EtOH. The radiochemical yield was 57.8% ± 7.6% (decay-uncorrected), and the radiochemical purity was greater than 98.8%.
5
Radiolabeling and Purification of [¹²⁴I]-5⁻
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Dry 124I-[5 ]− obtained from the previous step was dissolved in 250 μL of methanol. Sodium methoxide (2 mg) was added and stirred at room temperature for 6 h. After Complete hydrolysis, confirmed by analytical radio-HPLC, reformulation was carried out by dilution with water, retention on a C-18 cartridge (Sep-Pak® Light, Waters), further elution with ethanol (500 μL, Sigma-Aldrich) and evaporation to dryness. Quality control was performed by HPLC. Analytical conditions were: Stationary phase: Mediterranea Sea18 column (4.6 × 150 mm, 5 μm particle size, Teknokroma); mobile phase A: 0.1 M ammonium formate (AMF) buffer pH = 3.9; B: acetonitrile; flow rate = 1 mL/min; gradient: 0 min: 60% A- 40% B; 2 min: 60% A- 40% B; 6 min: 20% A- 80% B; 14 min: 0% A- 100% B; 16 min: 0% A- 100% B; 18 min: 60% A- 40% B; 20 min: 60% A- 40% B (retention time: 13.1 min).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!