TiO2-NP diameters were obtained from the analysis of transmission electron microscopy (TEM) images acquired with a FEI Tecnai G2 F20 S-TWIN HR(S) TEM equipped with an energy-dispersive X-ray spectroscopy (EDX) detector, operated at an accelerated voltage of 200 kV. Microliters of the samples were prepared by drop-casting 10 μL of the sample on a carbon-coated copper TEM grid and leaving to dry at room temperature. In addition, scanning electron microscopy was done with a FEI Magellan 400L XHR SEM, in scanning mode, operated at 1 kV, and in transmission mode, operated at 20 kV/STEM, for bigger sizes. The average size and size distribution of the samples were measured by using ImageJ software, by counting at least 300 particles from different regions of the grid. TEM images of Ag- and ZnO-NPs were acquired, but the size distribution was not measured.
Magellan 400l xhr sem
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Magellan 400L XHR SEM is a high-resolution scanning electron microscope designed for advanced materials analysis. It provides high-quality imaging and analytical capabilities for a wide range of samples.
Automatically generated - may contain errors
Lab products found in correlation
Tecnai g2 f20 s twin hr s tem, by Thermo Fisher Scientific (1 mentions)
Formvar coated 200 mesh copper grid, by Ted Pella (1 mentions)
Quanta 650 feg esem, by Thermo Fisher Scientific (1 mentions)
Glutaraldehyde, by Thermo Fisher Scientific (1 mentions)
Magnesium chloride mgcl2, by Merck Group (1 mentions)
Potassium hexacyanoferrate 2 trihydrate k4fe cn 6, by Merck Group (1 mentions)
Mgcl2, by Merck Group (1 mentions)
Sucrose, by Merck Group (1 mentions)
7 protocols using magellan 400l xhr sem
1
Characterization of TiO2 Nanoparticles
2
Visualizing Gold Nanoparticle-Antibody Complexes
3
Characterization of Nanostructured Films
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For the
morphological and elemental characterization of the generated nanostructured
films, a Magellan 400L XHR SEM (FEI, Hillsboro, OR) instrument and
a Quanta 650 FEG ESEM (FEI, Hillsboro, OR) instrument were used for
scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy
(EDX) analysis. The MNP size distribution (calculated on an average
number of 200 MNPs) was evaluated with “ImageJ, v.1.49.p”.
X-ray photoelectron spectroscopy (XPS) measurements were performed
using a SPECS PHOIBOS 150 hemispherical analyzer (SPECS GmbH, Berlin,
Germany), with a base pressure of 5 × 10–10 mbar, using a monochromatic Al K-alpha radiation (1486.74 eV) as
the excitation source. The XPS spectral deconvolution and the identification
of the peaks were run—following the technique described by
Sen et al.31 (link)—through the Gaussian–Lorentzian
fitting method,
after smoothing and subtraction of the Shirley-shaped background.
A confocal Raman spectrometer alpha300r (WITec, Ulm, German) equipped
with a 488 nm laser, using a 1.5 mW laser power, a grating 600 g/nm,
and objective 50×, was employed, using an exposure time of 10
s and three accumulations for each spectrum.
morphological and elemental characterization of the generated nanostructured
films, a Magellan 400L XHR SEM (FEI, Hillsboro, OR) instrument and
a Quanta 650 FEG ESEM (FEI, Hillsboro, OR) instrument were used for
scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy
(EDX) analysis. The MNP size distribution (calculated on an average
number of 200 MNPs) was evaluated with “ImageJ, v.1.49.p”.
X-ray photoelectron spectroscopy (XPS) measurements were performed
using a SPECS PHOIBOS 150 hemispherical analyzer (SPECS GmbH, Berlin,
Germany), with a base pressure of 5 × 10–10 mbar, using a monochromatic Al K-alpha radiation (1486.74 eV) as
the excitation source. The XPS spectral deconvolution and the identification
of the peaks were run—following the technique described by
Sen et al.31 (link)—through the Gaussian–Lorentzian
fitting method,
after smoothing and subtraction of the Shirley-shaped background.
A confocal Raman spectrometer alpha300r (WITec, Ulm, German) equipped
with a 488 nm laser, using a 1.5 mW laser power, a grating 600 g/nm,
and objective 50×, was employed, using an exposure time of 10
s and three accumulations for each spectrum.
4
Imaging and Analysis of Bacterial Nanocellulose Films
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Fragments of dry BNC films were placed flat on aluminium SEM sample holders with adhesive carbon tape. Metalized samples were sputtered with 5 nm of platinum before imaging. A high-resolution scanning electron microscope (FEI Magellan 400L XHR SEM) was used under a high vacuum and with an acceleration voltage of 2 kV to obtain images at 5.000, 20.000 and 100.000X magnifications.
Working distance was 4 mm and the current was 0.1 nA. Energy-dispersive X-ray (EDX) spectroscopy was performed with the same equipment at 1 KeV. At least two areas from four independent BNC samples were imaged on both sides of the films.
Working distance was 4 mm and the current was 0.1 nA. Energy-dispersive X-ray (EDX) spectroscopy was performed with the same equipment at 1 KeV. At least two areas from four independent BNC samples were imaged on both sides of the films.
5
Transcytosis of Fluorescent Nanoparticles Across BBB
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To perform this assay, AuNPs@POM@PEG and AuNPs@PEG were previously labelled fluorescently with Alexa647. Before the injection of the nanoparticles, time 0 for the BBB-oC with the cell medium into channels was set, with the corresponding fluorescent settings. Each nanoparticle sample was then resuspended in cell media with a final 2.5 nM concentration (to achieve a good fluorescent signal) and added into the blood channel via independent devices. Then, the BBB-oC was placed into the fluorescent microscope (Nikon Ti2) and fluorescent images were captured until the end point of 1h. The recorded pictures were analyzed using Fiji/ImageJ® software, and the permeability coefficients (cm/s) were determined following the equation from Campisi et al. [77 (link)].
For TEM visualization, AuNPs@POM@PEG were administered in the blood channel and incubated for 24 h at 37 °C and 5% CO2. Next, the channels were washed twice with PBS 1× to remove any nanoparticles that did not cross the BBB. The hydrogel was dissolved adding TRIzol reagent in both channels and incubated at 60 °C for 15 min. After that, the liquid in the main chamber could be collected. Holey carbon grids were prepared via depositing a droplet of the dissolved hydrogel onto them and letting them dry. Finally, the grids were observed with an FEI Magellan 400L XHR SEM, and EDX was performed on the region of interest.
For TEM visualization, AuNPs@POM@PEG were administered in the blood channel and incubated for 24 h at 37 °C and 5% CO2. Next, the channels were washed twice with PBS 1× to remove any nanoparticles that did not cross the BBB. The hydrogel was dissolved adding TRIzol reagent in both channels and incubated at 60 °C for 15 min. After that, the liquid in the main chamber could be collected. Holey carbon grids were prepared via depositing a droplet of the dissolved hydrogel onto them and letting them dry. Finally, the grids were observed with an FEI Magellan 400L XHR SEM, and EDX was performed on the region of interest.
6
SEM Imaging of Blood Coagulation Samples
7
Transcytosis of Fluorescent Nanoparticles Across BBB
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To perform this assay, AuNPs@POM@PEG and AuNPs@PEG were previously labelled fluorescently with Alexa647. Before the injection of the nanoparticles, time 0 for the BBB-oC with the cell medium into channels was set, with the corresponding fluorescent settings. Each nanoparticle sample was then resuspended in cell media with a final 2.5 nM concentration (to achieve a good fluorescent signal) and added into the blood channel via independent devices. Then, the BBB-oC was placed into the fluorescent microscope (Nikon Ti2) and fluorescent images were captured until the end point of 1h. The recorded pictures were analyzed using Fiji/ImageJ® software, and the permeability coefficients (cm/s) were determined following the equation from Campisi et al. [77 (link)].
For TEM visualization, AuNPs@POM@PEG were administered in the blood channel and incubated for 24 h at 37 °C and 5% CO2. Next, the channels were washed twice with PBS 1× to remove any nanoparticles that did not cross the BBB. The hydrogel was dissolved adding TRIzol reagent in both channels and incubated at 60 °C for 15 min. After that, the liquid in the main chamber could be collected. Holey carbon grids were prepared via depositing a droplet of the dissolved hydrogel onto them and letting them dry. Finally, the grids were observed with an FEI Magellan 400L XHR SEM, and EDX was performed on the region of interest.
For TEM visualization, AuNPs@POM@PEG were administered in the blood channel and incubated for 24 h at 37 °C and 5% CO2. Next, the channels were washed twice with PBS 1× to remove any nanoparticles that did not cross the BBB. The hydrogel was dissolved adding TRIzol reagent in both channels and incubated at 60 °C for 15 min. After that, the liquid in the main chamber could be collected. Holey carbon grids were prepared via depositing a droplet of the dissolved hydrogel onto them and letting them dry. Finally, the grids were observed with an FEI Magellan 400L XHR SEM, and EDX was performed on the region of interest.
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