The largest database of trusted experimental protocols

Antibody against β actin and hrp labeled secondary antibodies

Manufactured by Merck Group
Sourced in United States

Antibody against β-Actin and HRP-labeled secondary antibodies are laboratory reagents used for the detection and quantification of β-Actin, a cellular structural protein, in biological samples. The primary antibody specifically binds to β-Actin, while the secondary antibody, labeled with horseradish peroxidase (HRP), binds to the primary antibody, enabling visualization and measurement of the target protein.

Automatically generated - may contain errors

3 protocols using antibody against β actin and hrp labeled secondary antibodies

1

Immunoblotting Analysis of ERK5 Pathway

Check if the same lab product or an alternative is used in the 5 most similar protocols
DCA was from Santa Cruz Technologies. Galactose and glutamine were from GIBCO. Human anti-LDLR-PE and IgG were from BD Biosciences and 7AAD from Beckman. The MEK5 inhibitor BIX02189 and the ERK5 inhibitor XMD8–92 were from Selleck. RIPA buffer to prepare protein extracts was from Euromedex. The complete protease inhibitor cocktail (Complete EDTA-free) and the phosphatase inhibitor cocktail (PhosSTOP) were from Roche. ERK5 and MEF2A/C antibodies were from Cell Signaling Technology and Abcam respectively. The antibody against β-Actin and HRP-labeled secondary antibodies were from Sigma.
+ Open protocol
+ Expand
2

Investigating CD36 and LRP1 Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
DCA was from Santa Cruz Technologies. Galactose and glutamine were from GIBCO. Human anti-CD36-PE, anti-CD36L-PE, anti-LPR1-PE and IgG were from Beckton Dickinson and 7AAD from Beckman (Brea, CA, USA). The MEK5 inhibitor BIX02189 and the ERK5 inhibitor XMD8–92 were from Selleck Chemicals (Radnor, PA, USA). RIPA buffer to prepare protein extracts was from Euromedex (Souffelweyersheim, France). The complete protease inhibitor cocktail (Complete EDTA-free) and the phosphatase inhibitor cocktail (PhosSTOP) were from Roche (Bale, Switzerland). ERK5, ACDVL and MEF2A/C antibodies were from Cell Signaling Technology (Danvers, MA, USA) and Abcam (Cambridge, UK), respectively. The antibody against β-Actin and HRP-labeled secondary antibodies were from Sigma (Burlington, MA, USA).
+ Open protocol
+ Expand
3

Analyzing Cellular Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
DCA was from Santa Cruz Technologies. Galactose and glutamine were from GIBCO. RIPA buffer to prepare protein extracts was from Euromedex. The complete protease inhibitor cocktail (Complete EDTA-free) and the phosphatase inhibitor cocktail (PhosSTOP) were from Roche. H2O2, DMF and MMS were from Sigma. ERK5 and NRF2 antibodies were from Cell Signaling Technology and Santa Cruz respectively. The antibody against β-Actin and HRP-labeled secondary antibodies were from Sigma.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!