The hybridoma of mAb MHM23 (β2 integrin heterodimer specific) [15] (link) was a gift from Prof. AJ McMichael (John Radcliffe Hospital, UK). The hybridoma of conformation reporter mAb KIM127 [16] (link) was kindly provided by Dr. MK Robinson (UCB, CellTech, UK). mAbs were purified from respective hybridoma supernatant using Hi-Trap protein G/A columns (GE Healthcare Life Sciences). mAb 7E3 (β3 functional blocker) was obtained from Prof. Barry Coller (Rockefeller University, NY, USA). mAb HIP8 (anti-αIIb mAb) was purchased from eBioscience. FITC-conjugated sheep anti-mouse IgG and fibrinogen from human plasma were both purchased from Sigma.
Fitc conjugated sheep anti mouse igg
Manufactured by Merck Group
The FITC-conjugated sheep anti-mouse IgG is a laboratory reagent used for the detection and visualization of mouse immunoglobulin G (IgG) molecules. It consists of a fluorescein isothiocyanate (FITC) dye conjugated to sheep-derived antibodies that specifically bind to mouse IgG. This product is commonly used in various immunological techniques, such as immunofluorescence assays and flow cytometry, to facilitate the identification and localization of target proteins or cells expressing mouse IgG.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using fitc conjugated sheep anti mouse igg
1
Purification and Characterization of Integrin-Specific Monoclonal Antibodies
2
Immunofluorescence Assay for CXCR4 Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were plated onto Millicell EZ SLIDE 8-well glass (Millipore, Darmstadt, Germany) for 12 h and then fixed with 4% paraformaldehyde followed by permeabilization with phosphate buffered saline (PBS) containing 0.1% Triton X-100 (PBST). After blocking with normal goat serum, the cells were incubated with anti-CXCR4 antibodies (R&D Systems) at 4 °C overnight. After three washes with 0.1% PBST, the cells were incubated with secondary FITC-conjugated sheep anti-mouse IgG (Sigma) and mounted with DAPI (Sigma) after wash with PBST. The cellular localization and expression of CXCR4 were examined under a confocal microscope (Olympus, Tokyo, Japan).
3
Immunofluorescence Microscopy of Cytoskeleton
4
Porcine Ileum Cryosection Immunofluorescence
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cryosections (8 m) of porcine ileum obtained from 3-week-old piglets or from the gut loops were cut with a Leica CM3050 S cryostat, mounted on APES-coated glass slides, dried (30 min, 40°C) and fixed in acetone during 10 min at -20°C. Subsequently, the cryosections were incubated during 30 min in ammonium chloride buffer (50 mM, pH 8) and washed thoroughly. All washing steps were carried out at RT in PBS. Fc receptors were blocked for 30 min at 37°C with PBS containing 10% sheep or goat serum. Binding of the VHH-MG or IMM013 was detected by incubation for 1 h at 37°C with a FITC-conjugated sheep anti-mouse IgG (10 µg/ml; Sigma; F2883) or goat antimouse IgG2a. Nuclei were counterstained with Hoechst (10 µg/ml). To assess endocytosis sections were stained with a rabbit anti-pan-cytokeratin antibody (Abcam, ab9377) and detected with a TexasRed-conjugated anti-rabbit IgG. The sections were washed in ultrapure water and mounted in mounting solution (DABCO). Tissues were imaged with a Leica DC 100 fluorescence microscope mounted with a Scion Corporation camera or a Leica confocal microscope. Images were analyzed and processed using Fiji.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!