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Mm00439616 m1

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Mm00439616_m1 is a TaqMan Gene Expression Assay designed for the detection and quantification of a target gene in mouse samples. The assay includes a FAM-labeled probe and gene-specific primers that enable sensitive and specific detection of the target transcript. This product is intended for research use only and not for use in diagnostic procedures.

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Lab products found in correlation

Abi 7900ht, by Thermo Fisher Scientific (2 mentions) Qs3 real time pcr system, by Thermo Fisher Scientific (2 mentions) Rnaeasy micro kit, by Qiagen (2 mentions) High capacity reverse transcription kit, by Thermo Fisher Scientific (2 mentions) Rnase h, by Promega (2 mentions) Tbx21 mm00450960 m1, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

IL-10 Mm00439616_m1 (3 citations)

3 protocols using mm00439616 m1

1

Mouse Gene Expression Analysis

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RNA was extracted using RNAeasy microkit (Qiagen) and reverse transcribed into cDNA using as High Capacity Reverse Transcription kit (Applied Biosystems) according to manufacturer’s instructions, followed by RNaseH (Promega) treatment for 30 min at 37°C. cDNA was analyzed for the expression of the following factors on the 7900HT ABI or QS3 real-time PCR systems (Applied Biosystems) using TaqMan primer probes to mouse Maf, Mm 02581355_s1; Il10, Mm00439616_m1; Tbx21, Mm00450960_m1; Ifng, Mm01168134_m1; Gata3, Mm00484683_m1; Il4, Mm00445260_m1; Rorc, Mm01261019_g1 and Il17a, Mm00439619_m1, Foxp3, Mm 00475162_m1, Il2, Mm 00434256_m1 and Il2ra, Mm 01340213_m1 all from Applied Biosystems. The comparative threshold cycle method with Hrpt1, Mm03024075_m1 as an internal control was used for the normalization of target gene expression.
Gabryšová L., Alvarez-Martinez M., Luisier R., Cox L.S., Sodenkamp J., Hosking C., Pérez-Mazliah D., Whicher C., Kannan Y., Potempa K., Wu X., Bhaw L., Wende H., Sieweke M.H., Elgar G., Wilson M., Briscoe J., Metzis V., Langhorne J., Luscombe N.M, & O’Garra A. (2018). c-Maf controls immune responses by regulating disease-specific gene networks and repressing IL-2 in CD4+ T cells. Nature immunology, 19(5), 497-507.
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2

Mouse Gene Expression Analysis

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RNA was extracted using RNAeasy microkit (Qiagen) and reverse transcribed into cDNA using as High Capacity Reverse Transcription kit (Applied Biosystems) according to manufacturer’s instructions, followed by RNaseH (Promega) treatment for 30 min at 37°C. cDNA was analyzed for the expression of the following factors on the 7900HT ABI or QS3 real-time PCR systems (Applied Biosystems) using TaqMan primer probes to mouse Maf, Mm 02581355_s1; Il10, Mm00439616_m1; Tbx21, Mm00450960_m1; Ifng, Mm01168134_m1; Gata3, Mm00484683_m1; Il4, Mm00445260_m1; Rorc, Mm01261019_g1 and Il17a, Mm00439619_m1, Foxp3, Mm 00475162_m1, Il2, Mm 00434256_m1 and Il2ra, Mm 01340213_m1 all from Applied Biosystems. The comparative threshold cycle method with Hrpt1, Mm03024075_m1 as an internal control was used for the normalization of target gene expression.
Gabryšová L., Alvarez-Martinez M., Luisier R., Cox L.S., Sodenkamp J., Hosking C., Pérez-Mazliah D., Whicher C., Kannan Y., Potempa K., Wu X., Bhaw L., Wende H., Sieweke M.H., Elgar G., Wilson M., Briscoe J., Metzis V., Langhorne J., Luscombe N.M, & O’Garra A. (2018). c-Maf controls immune responses by regulating disease-specific gene networks and repressing IL-2 in CD4+ T cells. Nature immunology, 19(5), 497-507.
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3

Quantitative RNA Expression Analysis

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qRT-PCR was performed using an ABI prism 7000 Sequence Detection System with TaqMan gene expression and premix assays for tumor necrosis factor (Tnf), Mm00443258_m1; Tfam, Mm0114667_m1; Ogg1, Mm01184571_g1; Nrf-1, Mm01135607_m1; NRF-2 (Gabpa) Mm00484597_m1, Neutrophil gelatinase-associated lipocalin (Ngal) Mm01324469_g1, Interleukin-10 (Il-10) Mm 00439616_m1, Peroxisome proliferator-activated receptor coactivator 1-a (Ppargc1a) Mm 00447187_g1 from Applied Biosystems, Foster City, Ca. 18S rRNA was used as an endogenous control. Quantification of gene expression was determined by the comparative threshold cycle CT and relative quantification (RQ) method.
Bartz R.R., Fu P., Suliman H.B., Crowley S.D., MacGarvey N.C., Welty-Wolf K, & Piantadosi C.A. (2014). Staphylococcus aureus Sepsis Induces Early Renal Mitochondrial DNA Repair and Mitochondrial Biogenesis in Mice. PLoS ONE, 9(7), e100912.
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