A venous blood sample was taken approximately 15 min after the experiment at a pathology service located within walking distance from the University by registered nurses trained in venepuncture techniques; all procedures were conducted taking standard infection control precautions. Serum estradiol concentrations were analyzed by Healthscope Pathology Services using an ADVIA Centaur Enhanced Estradiol assay (Siemens), which is a competitive assay that measures serum estradiol concentrations up to 3000 pg/mL (11,010 pmol/L) with a limit of detection of 11.8 pg/mL (43.6 pmol/L). Progesterone levels were analyzed using an ADVIA Centaur Progesterone assay (Siemens), which is a competitive immunoassay that measures serum progesterone concentration up to 60 ng/mL (190.8 nmol/L) with a minimum detectable concentration of 0.21 ng/mL (0.67 nmol/L).
Advia centaur progesterone assay
Manufactured by Siemens
The ADVIA Centaur Progesterone assay is a laboratory instrument designed to measure the concentration of the hormone progesterone in blood samples. It utilizes chemiluminescent technology to detect and quantify the level of progesterone present.
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Lab products found in correlation
2 protocols using advia centaur progesterone assay
1
Serum Hormone Concentration Analysis
2
Evaluating Menstrual Cycle Hormone Profiles
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A venous blood sample was drawn from each female participant on Day 1 of the experiment at a pathology service located within walking distance from the University.
Serum hormone concentrations were analyzed by Healthscope Pathology Services.
Estradiol levels were analyzed using an ADVIA Centaur Enhanced Estradiol assay (Siemens), which is a competitive assay that measures serum estradiol concentrations up to 3000 pg/mL (11,010 pmol/L) with a limit of detection of 11.8 pg/mL (43.6 pmol/L).
Progesterone levels were analyzed using an ADVIA Centaur Progesterone assay (Siemens), which is a competitive immunoassay that measures serum progesterone concentration up to 60 ng/mL (190.8 nmol/L) with a minimum detectable concentration of 0.21 ng/mL (0.67 nmol/L). Naturally cycling women were invited to participate in the experiment across all phases of the menstrual cycle to achieve wide variance in estradiol and progesterone levels.
The experimenter remained blind to the results of serum analyses for each participant until after the completion of the two-day experimental procedure.
Serum hormone concentrations were analyzed by Healthscope Pathology Services.
Estradiol levels were analyzed using an ADVIA Centaur Enhanced Estradiol assay (Siemens), which is a competitive assay that measures serum estradiol concentrations up to 3000 pg/mL (11,010 pmol/L) with a limit of detection of 11.8 pg/mL (43.6 pmol/L).
Progesterone levels were analyzed using an ADVIA Centaur Progesterone assay (Siemens), which is a competitive immunoassay that measures serum progesterone concentration up to 60 ng/mL (190.8 nmol/L) with a minimum detectable concentration of 0.21 ng/mL (0.67 nmol/L). Naturally cycling women were invited to participate in the experiment across all phases of the menstrual cycle to achieve wide variance in estradiol and progesterone levels.
The experimenter remained blind to the results of serum analyses for each participant until after the completion of the two-day experimental procedure.
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