The oral treatment with a recombinant CCL11 (R&D Systems, Minneapolis, MN, USA) was performed as previously described with modifications (27 (link)). Briefly, groups of sensitized mice were deprived of food for 2 h, followed by intragastric administration of an isotonic bicarbonate solution (8 parts HBSS and 2 parts 7.5% sodium bicarbonate) to neutralize stomach acidity. Individual mice were gavaged with a 0.1 ml PBS solution (pH 7.2) containing 1 µg of recombinant CCL11 and 30 min later, were gavaged with 50 mg of OVA in 0.5 ml of PBS.
Recombinant ccl11
Manufactured by R&D Systems
Sourced in United States
Recombinant CCL11 is a protein produced in a laboratory setting. It functions as a chemokine, which is a type of signaling molecule that helps coordinate the movement and activation of immune cells. The core function of Recombinant CCL11 is to facilitate these cellular processes, though its specific applications and intended uses are not included in this description.
Automatically generated - may contain errors
2 protocols using recombinant ccl11
1
Oral CCL11 Sensitization and OVA Challenge
2
C2C12 Myoblast Differentiation Assay
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Mouse C2C12 myoblasts were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) and maintained in Dulbecco’s Modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum, 20 mM hydroxyethyl piperazine ethane sulfonic acid, 2 mM L-glutamine, 100 U/mL penicillin, and 0.1 mg/mL streptomycin (Life Technologies Corp., Carlsbad, CA, USA) at 37°C in a humidified atmosphere containing 5% CO2. To induce myogenesis, cells were grown to 90% confluency in maintenance media and switched to differentiation media (DMEM with 2% horse serum) for 3 or 4 days of culture. Recombinant CCL11 was purchased from R&D Systems (cat. no. 420-ME-100; Minneapolis, MN, USA).
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