An undifferentiated human iPSC line (Thermo Fisher Scientific) was routinely maintained in complete Essential 8 (E8) cell culture medium (Thermo Fisher Scientific) on 6-well plates coated with 1 : 100 growth factor–reduced Matrigel (R&D Systems). The cells were passaged every 3–4 days using 0.5 mM EDTA in D-PBS (Thermo Fisher Scientific) and re-plated in E8 medium supplemented with 2 μM of the ROCK inhibitor Thiazovivin (Stratech Scientific) for the first 24 h following passaging. Human cardiac microvascular endothelial cells (hCMEC; PromoCell) were grown in PromoCell Cell Growth Medium and passaged using PromoCell DetachKit. The media was changed every two to three days. Human cardiac fibroblasts (hCFib; kindly provided by Prof. Terracciano) were cultured in DMEM high glucose (Thermo Fisher Scientific) supplement with 10% (v/v) FBS (Thermo Fisher Scientific) and dissociated using 0.25% trypsin (Sigma-Aldrich).
Complete essential 8 cell culture medium
Manufactured by Thermo Fisher Scientific
The Complete Essential 8 (E8) cell culture medium is a defined, serum-free medium designed for the culture of human embryonic stem cells and induced pluripotent stem cells. It provides the essential nutrients and growth factors required to maintain the undifferentiated state of these cells in culture.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco phosphate buffered saline (dpbs), by Thermo Fisher Scientific (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Hcmec, by PromoCell (2 mentions)
Human ipsc line, by Thermo Fisher Scientific (2 mentions)
Growth factor reduced matrigel, by R&D Systems (2 mentions)
High glucose dmem, by Thermo Fisher Scientific (2 mentions)
Trypsin, by Merck Group (2 mentions)
Detachkit, by PromoCell (2 mentions)
2 protocols using complete essential 8 cell culture medium
1
Culturing iPSCs, hCMECs, and hCFibs
2
Culturing iPSCs, hCMECs, and hCFibs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
An undifferentiated human iPSC line (Thermo Fisher Scientific) was routinely maintained in complete Essential 8 (E8) cell culture medium (Thermo Fisher Scientific) on 6-well plates coated with 1 : 100 growth factor–reduced Matrigel (R&D Systems). The cells were passaged every 3–4 days using 0.5 mM EDTA in D-PBS (Thermo Fisher Scientific) and re-plated in E8 medium supplemented with 2 μM of the ROCK inhibitor Thiazovivin (Stratech Scientific) for the first 24 h following passaging. Human cardiac microvascular endothelial cells (hCMEC; PromoCell) were grown in PromoCell Cell Growth Medium and passaged using PromoCell DetachKit. The media was changed every two to three days. Human cardiac fibroblasts (hCFib; kindly provided by Prof. Terracciano) were cultured in DMEM high glucose (Thermo Fisher Scientific) supplement with 10% (v/v) FBS (Thermo Fisher Scientific) and dissociated using 0.25% trypsin (Sigma-Aldrich).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!