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Primary antibodies against pdgfrα

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Primary antibodies against PDGFRα are laboratory reagents used to detect the presence and quantify the levels of the Platelet-Derived Growth Factor Receptor Alpha protein in biological samples. These antibodies specifically bind to the PDGFRα protein and can be used in various immunoassay techniques.

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Lab products found in correlation

Tyramide superboost kit, by Thermo Fisher Scientific (2 mentions)

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PDGFRα (13 citations)

2 protocols using primary antibodies against pdgfrα

1

Immunofluorescence Staining of PDGFRα

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For the immunofluorescence staining of PDGFRα (Figure 3H), slides were blocked with 3% H2O2 for 15 minutes following fixation to inhibit endogenous peroxidases. Sections were the blocked for 1 hour with blocking buffer at RT. Tissues were incubated with primary antibodies against PDGFRα (1:300, eBioscience) for 3 hours, washed with 1X PBS, and stained with secondary antibodies for 3 hours (1:200) A Tyramide SuperBoost kit (Invitrogen) was used for the detection of PDGFRα per manufacturer instructions. For PDGFRα staining in Figure 4A, sections were stained with antibodies against PDGFRα (1:200, R and D systems) diluted in blocking buffer for 3 hours at RT, followed by staining with corresponding secondary antibody for 1 hour at RT.
Kastenschmidt J.M., Coulis G., Farahat P.K., Pham P., Rios R., Cristal T.T., Mannaa A.H., Ayer R.E., Yahia R., Deshpande A.A., Hughes B.S., Savage A.K., Giesige C.R., Harper S.Q., Locksley R.M., Mozaffar T, & Villalta S.A. (2021). A stromal progenitor and ILC2 niche promotes muscle eosinophilia and fibrosis-associated gene expression. Cell reports, 35(2), 108997.
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2

Immunofluorescence Staining of PDGFRα

Check if the same lab product or an alternative is used in the 5 most similar protocols
For the immunofluorescence staining of PDGFRα (Figure 3H), slides were blocked with 3% H2O2 for 15 minutes following fixation to inhibit endogenous peroxidases. Sections were the blocked for 1 hour with blocking buffer at RT. Tissues were incubated with primary antibodies against PDGFRα (1:300, eBioscience) for 3 hours, washed with 1X PBS, and stained with secondary antibodies for 3 hours (1:200) A Tyramide SuperBoost kit (Invitrogen) was used for the detection of PDGFRα per manufacturer instructions. For PDGFRα staining in Figure 4A, sections were stained with antibodies against PDGFRα (1:200, R and D systems) diluted in blocking buffer for 3 hours at RT, followed by staining with corresponding secondary antibody for 1 hour at RT.
Kastenschmidt J.M., Coulis G., Farahat P.K., Pham P., Rios R., Cristal T.T., Mannaa A.H., Ayer R.E., Yahia R., Deshpande A.A., Hughes B.S., Savage A.K., Giesige C.R., Harper S.Q., Locksley R.M., Mozaffar T, & Villalta S.A. (2021). A stromal progenitor and ILC2 niche promotes muscle eosinophilia and fibrosis-associated gene expression. Cell reports, 35(2), 108997.
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