Anti anxa1

The following primary antibodies were used in this study: anti-TNFR1 (1:2,000, rabbit, Abcam), anti-ANXA1 (1:1,000, goat, Santa Cruz), anti-Ki-67 (1:50, mouse, Abcam), anti-P65 (1:2,000, rabbit, Cell Signaling Technology), anti-p-P65 (1:1,000, rabbit, Cell Signaling Technology), anti-Akt (1:2,000, rabbit, Cell Signaling Technology), anti-p-Akt (S473) (1:1,000, rabbit, Cell Signaling Technology), anti-p-Akt (T308) (1:1,000, rabbit, Cell Signaling Technology), anti-GAPDH (1:5,000, mouse, Abcam), anti-α-tubulin = (1:5,000, mouse, Abcam), anti-Lamin B (1:2,000, mouse, Santa Cruz), anti-HA (1:5,000, mouse, Cell Signaling Technology), and anti-Flag (1:5,000, rabbit, Cell Signaling Technology).

Db-cAMP, H89, staurosporine, CFSE and LPS (from Escherichia coli serotype O:111:B4) were from Sigma-Aldrich (San Luis, MO, USA); IFN-γ and IL-4 were from Biolegend (San Diego, CA, USA); RS504393 (Tocris, Bristol, England, UK); western blot antibodies were from Sigma (β-actin), Cell Signaling Technology (Danvers, MA, USA; STAT1, p-STAT1, p-STAT3, secondary anti-rabbit peroxidase conjugate antibody) or Santa Cruz Biotechnology (Dallas, TX, USA; secondary anti-mouse peroxidase conjugate antibody); ELISA kits for measurement of IL-10, TGF-β, CCL2, IL-6 and TNF-α were from R&D Systems (Minneapolis, MN, USA). The fluorescent monoclonal antibodies were anti-F4/80 (PE-Cy7 or APC, eBioscience, San Diego, CA, USA), anti-GR1 (PE, eBioscience), anti-CD11b (alexa fluor 488, Biolegend, San Diego, CA, USA and V500, Pharmingen), anti-rabbit secondary (Alexa fluor 488 Cell Signaling, Danvers, MA, USA), anti-AnxA1 (Santa Cruz Biotechnology), anti-Ly6C (PeCy7, Biolegend), anti-Ly6G (APCCy7 or BV421, Biolegend), anti-CD36 (APC, BD biosciences) and anti-CD3 (FITC, Pharmingen).