Anti-Mo CD3e (2074540; 1:100 dilution) and anti-Mo CD8a (2075802; 1:100 dilution) antibodies were purchased from Invitrogen (CA, USA). Alexa Fluor® 647 (B319823; 1:100 dilution) was purchased from BioLegend (CA, USA). Anti-CD8 (ab4055; 1:500 dilution) was purchased from Abcam (Illinois, USA). Anti-RNF125 (A15166; 1:1,000 dilution) was purchased from ABclonal (Wuhan, China). Anti-α-tubulin antibody (T6074; 1:5000 dilution) and anti-Flag (A8592; 1:5000 dilution) antibody were purchased from Sigma–Aldrich (Missouri, USA). Anti-PD-L1 (17952-1-AP; 1:1,000 dilution) and anti-Myc (16286-1-AP; 1:1,000 dilution) antibodies were purchased from Proteintech (Wuhan, China). Anti-HA (14031S; 1:1,000 dilution) was purchased from Cell Signaling Technology (Danvers, USA). Anti-rabbit HRP-IgG (ZB-2301; 1:5000 dilution) and anti-mouse HRP-IgG (ZB-2305; 1:5000 dilution) secondary antibodies were purchased from ZSGB-BIO (Beijing, China). CD4 (GB11064; 1:1000 dilution), CD8 (GB114196; 1:1000 dilution) and F4/80 (GB113373; 1:1000 dilution) antibodies were purchased from Servicebio (WuHan, China).
Cd8 gb114196
Manufactured by Wuhan Servicebio Technology
Sourced in China
CD8 (GB114196) is a lab equipment product designed for detecting and analyzing CD8 T cells. It is a tool used in research and clinical applications. The product's core function is to facilitate the identification and quantification of CD8 T cells, which play a crucial role in the immune system.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor 647, by BioLegend (1 mentions)
Anti flag a8592, by Merck Group (1 mentions)
Anti myc 16 286 1 ap, by Proteintech (1 mentions)
Anti cd8 ab4055, by Abcam (1 mentions)
F4 80 gb113373, by Wuhan Servicebio Technology (1 mentions)
Anti α tubulin antibody t6074, by Merck Group (1 mentions)
Fluorescence microscope, by Leica (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions)
2 protocols using cd8 gb114196
1
Multiparameter Immune Cell Profiling
2
Immunohistochemical Phenotyping of Tumor-Associated Immune Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The tissue sections were placed in a pressure cooker filled with citrate antigen retrieval buffer (Servicebio, China). After natural cooling, the slides were washed 3 times with PBS for 3 min each time. After serum blocking, sections were incubated with the following primary antibodies overnight at 4 °C: CD4 (GB13064-2, Servicebio) and CD8 (GB114196, Servicebio) to detect T cells; CD86 (19589S, CST) and CD206 (24595S, CST) to detect TAMs, washed 3 times with PBS, incubated with HRP-conjugated or fluorescently conjugated secondary antibodies at room temperature for 50 min, and labelled with DAPI (Beyotime, China). Finally, the slides were mounted with neutral gum and observed, and pictures were taken under a fluorescence microscope (Leica, Germany).
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