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Antibodies to mouse tlr7

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Antibodies to mouse TLR7 are laboratory reagents used for the detection and study of the toll-like receptor 7 (TLR7) protein in mouse biological samples. TLR7 is an important component of the innate immune system, involved in the recognition of viral RNA. These antibodies can be used for techniques such as Western blotting, immunohistochemistry, and flow cytometry to investigate the expression and localization of TLR7 in mouse models.

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Lab products found in correlation

Lamp1, by Thermo Fisher Scientific (2 mentions) Myd88, by Thermo Fisher Scientific (2 mentions) Alexfluor550, by Thermo Fisher Scientific (2 mentions) Confocal microscope, by Nikon (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Confocal dishes, by MatTek (2 mentions) Alexa fluor 488, by Thermo Fisher Scientific (2 mentions)

2 protocols using antibodies to mouse tlr7

1

Intracellular Localization of TLR7 Signaling Components in BMDCs

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BMDCs were seeded in confocal dishes (Mattek) at a final concentration of 1×105 cells/mL in RPMI-complete media. The following day, cells were treated with 10 μg/mL GRD for 5 minutes and 15 minutes at 37C. Cells were carefully washed with PBS and fixed with 2% PFA for 10 minutes at room temperature in the dark. Fixed cells were permeabilized with SAP buffer (2% saponin in PBS) for 1 hour at 37C. Following permeabilization, BMDCs were intracellularly stained with antibodies to mouse TLR7, MyD88, EEA1, or LAMP1 (Thermo Scientific), followed by staining with secondary antibodies fluorescently labeled with AlexaFluor488 or AlexFluor550 (Invitrogen). Cells were washed 3 times with PBS. Finally, DNA was stained with 1:1000 dilution of DAPI (Invitrogen) for 10 minutes at RT. Cells were visualized using a Nikon confocal microscope and analysis performed using ZEN software. Data analysis and processing of images was conducted using ImageJ software (NIH).
Ramirez-Ortiz Z.G., Prasad A., Griffith J.W., Pendergraft WF I.I.I., Cowley G.S., Root D.E., Tai M., Luster A.D., Khoury J.E., Hacohen N, & Means T.K. (2015). TREML4 amplifies TLR7-mediated signaling during antiviral responses and autoimmunity. Nature immunology, 16(5), 495-504.
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2

Intracellular Localization of TLR7 Signaling Components in BMDCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
BMDCs were seeded in confocal dishes (Mattek) at a final concentration of 1×105 cells/mL in RPMI-complete media. The following day, cells were treated with 10 μg/mL GRD for 5 minutes and 15 minutes at 37C. Cells were carefully washed with PBS and fixed with 2% PFA for 10 minutes at room temperature in the dark. Fixed cells were permeabilized with SAP buffer (2% saponin in PBS) for 1 hour at 37C. Following permeabilization, BMDCs were intracellularly stained with antibodies to mouse TLR7, MyD88, EEA1, or LAMP1 (Thermo Scientific), followed by staining with secondary antibodies fluorescently labeled with AlexaFluor488 or AlexFluor550 (Invitrogen). Cells were washed 3 times with PBS. Finally, DNA was stained with 1:1000 dilution of DAPI (Invitrogen) for 10 minutes at RT. Cells were visualized using a Nikon confocal microscope and analysis performed using ZEN software. Data analysis and processing of images was conducted using ImageJ software (NIH).
Ramirez-Ortiz Z.G., Prasad A., Griffith J.W., Pendergraft WF I.I.I., Cowley G.S., Root D.E., Tai M., Luster A.D., Khoury J.E., Hacohen N, & Means T.K. (2015). TREML4 amplifies TLR7-mediated signaling during antiviral responses and autoimmunity. Nature immunology, 16(5), 495-504.
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