GFP-22 siRNA was purchased from Qiagen (USA) and scrambled siRNA (custom 22 mer) was purchased from GE Healthcare Dharmacon (USA) (Sense Strand: 5’- GCA AGC TGA CCC TGA AGT TC (dTdT)-3’, anti-sense strand 3’- GAA CUU CAG GGU CAG CUU GC-5’, Mol. Wt. 13,965.6 (g/mol), the oligonucleotide has been converted to the 2’-hydroxyl, and annealed), Inc. MTS reagent was purchased from Promega (USA). Lipofectamine® 2000 (L2K) transfection reagent, SYTOX® Red dead cell stain, LysoTracker® Blue DND-22, and AlexaFluor®680 Wheat Germ Agglutinin- (AlexaFluor® 680 WGA) conjugate were purchased from Life Technologies (USA). Label IT® siRNA Tracker intracellular localization kit (Cy3) was obtained from Mirus (USA). Fetal Bovine Serum (FBS), DMEM and RPMI media, sodium pyruvate, PBS and trypsin, were purchased from Atlanta Biologicals (USA). Mark-tubes made of borosilicate glass #50, (L= 80 OD= 1.50 Wall= 0.01 mm) were purchased from Hilgenberg GmbH (Germany).
Gfp 22 sirna
Manufactured by Qiagen
Sourced in United States, Germany
The GFP-22 siRNA is a laboratory tool designed for gene silencing experiments. It is a small interfering RNA (siRNA) molecule that targets the green fluorescent protein (GFP) gene, allowing for the study of gene function and regulation. The core function of the GFP-22 siRNA is to induce the degradation of GFP mRNA, effectively reducing the expression of the GFP protein in cells.
Automatically generated - may contain errors
Lab products found in correlation
Mts reagent, by Promega (1 mentions)
Sytox red dead cell stain, by Thermo Fisher Scientific (1 mentions)
Lysotracker blue dnd 22, by Thermo Fisher Scientific (1 mentions)
Trypsin, by Atlanta Biologicals (1 mentions)
Sodium pyruvate, by Atlanta Biologicals (1 mentions)
Lipofectamine ltx, by Thermo Fisher Scientific (1 mentions)
Lipofectamin rnaimax, by Thermo Fisher Scientific (1 mentions)
2 protocols using gfp 22 sirna
1
Quantifying Cellular Uptake and Localization
2
Rac1 and Rac1b Expression Modulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For Rac1 expression, H23 and HCC44 cells were transfected with pEGFP, pEGFP-Rac1 and pEGFP-Rac1b (Hage et al., 2009) with Lipofectamine LTX (ThermoFisher Scientific, Langenselbold, Germany) according to manufacturer's instructions. Cell lysates were analyzed 24-48 h post transfection. To generate stable EGFP-Rac1-/EGFP-Rac1bexpressing cell clones, transfected cells were selected with 1.5 mg/ml G418 (Capricorn). Resistant cells were subcloned and EGFP/EGFP-Rac1/EGFP-Rac1b expression was confirmed by western blot analyses and fluorescence microscopy (IX81, Olympus, Hamburg, Germany). Two clones per construct were chosen for further experiments.
For siRNA transfection, H23 and H358 cells were transfected with scrambled, non-targeting siRNA (Dharmafect ON-TARGET plus control pool) (Dharmacon, Lafayette, CO, USA), a mixture of Rac1bsi-1A and Rac1b-si-1B or GFP-22 siRNA (all Qiagen, Hilden, Germany), siESRP1_SMARTpool and siESRP2_SMARTpool (both Dharmacon) using Lipofectamin RNAiMax (ThermoFisher Scientific). The cells were transfected twice with siRNAs and lysed 72 h after the second transfection.
For siRNA transfection, H23 and H358 cells were transfected with scrambled, non-targeting siRNA (Dharmafect ON-TARGET plus control pool) (Dharmacon, Lafayette, CO, USA), a mixture of Rac1bsi-1A and Rac1b-si-1B or GFP-22 siRNA (all Qiagen, Hilden, Germany), siESRP1_SMARTpool and siESRP2_SMARTpool (both Dharmacon) using Lipofectamin RNAiMax (ThermoFisher Scientific). The cells were transfected twice with siRNAs and lysed 72 h after the second transfection.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!