On targetplus bet proteins sirnas

Manufactured by Horizon Discovery

ON-TARGETplus BET proteins siRNAs are a set of small interfering RNA (siRNA) designed to target specific BET (Bromodomain and Extra-Terminal) proteins. BET proteins play a role in regulating gene expression and are implicated in various disease processes. The ON-TARGETplus siRNAs are designed to provide efficient knockdown of BET protein expression, enabling researchers to study their biological functions.

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Lab products found in correlation

Lipofectamine 2000 reagent, by Thermo Fisher Scientific (4 mentions) Preceiver m01, by GeneCopoeia (2 mentions)

Spelling variants of this product

ON-TARGETplus (235 citations) ON-TARGETplus siRNAs (227 citations) SiRNAs (161 citations)

2 protocols using on targetplus bet proteins sirnas

BET Protein Expression and Knockdown

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Plasmids coding for His-tagged BET proteins, His-Jagged1 or control vector pReceiver-M01 were purchased from GeneCopoeia. ON-TARGETplus BET proteins siRNAs were obtained from Dharmacon and Jagged1 siRNA (sc-3702) from Santa Cruz Biotechnology. Cells were transfected with plasmids and siRNAs by Lipofectamine 2000 reagent (Thermo Fischer Scientific).

Andrieu G., Tran A.H., Strissel K.J, & Denis G.V. (2016). BRD4 regulates breast cancer dissemination through Jagged1/Notch1 signaling. Cancer research, 76(22), 6555-6567.

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BET Protein Modulation Protocol

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Plasmids coding for His-tagged BET proteins or control vector pReceiver-M01 were purchased from GeneCopoeia. ON-TARGETplus BET proteins siRNAs were obtained from Dharmacon. Cells were transfected with plasmids and siRNAs by Lipofectamine 2000 reagent (Thermo Fisher Scientific) as previously validated (16 (link)). Efficient depletions or overexpressions were obtained three days post-transfection.

Andrieu G.P, & Denis G.V. (2018). BET Proteins Exhibit Transcriptional and Functional Opposition in the Epithelial-to-mesenchymal Transition. Molecular cancer research : MCR, 16(4), 580-586.

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