S 6 methoxy 2 5 7 8 tetramethylchromane 2 carboxylic acid trolox

Grasshopper samples were taken from maize fields in Coronango, Puebla, Mexico. The geographical coordinates are the parallels 19^° 06′36″ and 19^° 10′42″ of north latitude and the meridians 98^° 14′54″ and 98^° 19′40″ of western longitude to 2,180 m above sea level. The recollection took place in September 2020 for early grasshopper (EG) and November 2020 for adult grasshopper (AG). Grasshoppers were collected and transported alive, and later, they were cleaned, not purged, and washed with distilled water, and frozen at −80^°C. The samples were freeze-dried, blended (NutriBullet NBR-0601), and stored at room temperature until use.
Folin–Ciocalteu reagent, gallic acid, 2,2-diphenyl-1-picrylhydrazyl (DPPH•), 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS•+), (S)-6-methoxy-2,5,7,8-tetramethylchromane-2-carboxylic acid trolox (Sigma Aldrich), serine endoprotease from Bacillus licheniformis 2.4L E.C.3.4.21.14 (Sigma Aldrich), Luria Agar, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Enterobacter aerogenes ATCC 13048 Salmonella sp., and Pseudomonas aeruginosa ATCC 77853 were used in this study. Samples of EG, AG, early grasshopper extract (EGE), adult grasshopper extract (AGE), early grasshopper hydrolysate (EGH), adult grasshopper hydrolysate (AGH), and hydrolyzed fractions were tested in this research.

Hydrochloric acid, bromocresol green and methyl red indicators, boric acid, Folin–Ciocalteu’s reagent and sodium carbonate were supplied by Kemika (Zagreb, Croatia). Kjeldahl tablets were purchased from CarlRoth (Karsruhe, Germany). An integrated total dietary fibre assay kit was purchased from Megazyme (Wicklow, Ireland). Gallic acid (>97%), rosmarinic acid (97%), caffeic acid (HPLC standard), cryptochlorogenic (>98%), chlorogenic acid (95%), rutin trihydrate (>97%), luteolin (>98%), apigenin (>97%), (S)-6-Methoxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Methanol was supplied from Panreac (Barcelona, Spain) and sulfuric acid, hexane, ethanol, formic acid and acetonitrile from Carlo Erba (Val de Reuil, France). Acetone was supplied by Gram-mol d.o.o (Zagreb, Croatia). All chemicals used for experimental procedures were of analytical grade or HPLC grade.

Petroleum ether was supplied from Kemika (Zagreb, Croatia). Folin–Ciocalteu reagent and sodium carbonate were supplied from Lach-ner (Neratovice, Czech Republic). Lead(II) acetate trihydrate, (S)-6-Methoxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,20-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), gallic acid (>97%), chlorogenic acid/3-O-caffeoylquinic acid (3-CQA; 95%), cryptochlorogenic acid/4-O-caffeoylquinic acid (4-CQA; >98%) and neochlorogenic acid/5-O-caffeoylquinic acid (5-CQA; >98%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Standards of sucrose and caffeine were purchased from Fluka (Taufkirchen, Germany). Ethanol and sodium oxalate were supplied from Gram-mol d.o.o. (Zagreb, Croatia), and acetonitrile, methanol and formic acid from Fisher Scientific (Hampton, NH, SAD). Caramel was supplied by Frutarom (Haifa, Israel). All chemicals used for experimental procedures were of analytical or HPLC grade.