Anti p vegfr2

Manufactured by Abcam

Sourced in United States

Anti-p-VEGFR2 is a protein marker that can be used to detect the phosphorylated form of the Vascular Endothelial Growth Factor Receptor 2 (VEGFR2) protein. VEGFR2 is an important receptor involved in the regulation of angiogenesis and vascular permeability.

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VEGFR2 (57 citations) Anti-VEGFR2 (25 citations) P-VEGFR2 (5 citations)

2 protocols using anti p vegfr2

Western Blot Analysis of Apoptosis and Signaling

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Cells were lysed using RIPA Lysis Buffer (Thermo Fisher Scientific) at 4°C. Proteins (30 μg per lane) were separated on 10% SDS-PAGE gel, and then transferred onto a polyvinylidene fluoride membrane (PVDF; Millipore, Billerica, MA, USA). Following this, the membrane was blocked with 5% non-fat milk for 1 hr at room temperature. Later on, the membrane was incubated with primary antibodies overnight at 4°C. Anti-cleaved caspase 3 (1:1000, Abcam Cambridge, MA, USA), anti-p-EGFR (1:1000, Abcam), anti-EGFR (1:1000, Abcam), anti-p-VEGFR2 (1:1000, Abcam), anti-VEGFR2 (1:1000, Abcam), anti-p-Akt (1:1000, Abcam), anti-Akt (1:1000, Abcam), anti-β-actin (1:1000, Abcam). After that, the membranes were incubated with HRP-goat anti-rabbit secondary antibody (1: 5000, Abcam) for 1 hr at room temperature. Enhanced chemiluminescence detection reagent (Tanon, Shanghai, China) was used to visualize the signal strength of the bands.

Wang R., Luo Z., Zhang H, & Wang T. (2019). Tanshinone IIA Reverses Gefitinib-Resistance In Human Non-Small-Cell Lung Cancer Via Regulation Of VEGFR/Akt Pathway. OncoTargets and therapy, 12, 9355-9365.

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Western Blot Analysis of Angiogenic Factors

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Retinas and cells were lysed in radio immune precipitation assay (RIPA)
buffer with protease inhibitor mixture, PMSF, and sodium orthovanadate (Santa
Cruz Biotechnology, Santa Cruz, CA). Twenty-five µg of proteins were
resolved by SDS-PAGE and then blotted with specific antibodies: anti-Nox4,
anti-VEGF (Santa Cruz Biotechnology, Santa Cruz, CA), anti-VEGFR2,
anti-phospho-p44/42 MAPK (Erk1/2) (Cell Signaling Technology, Boston, MA),
anti-p-VEGFR2, anti-angiopoietin-2, anti-integrin αV (Abcam, Cambridge,
MA). The same membrane was stripped and reblotted with an anti-β-actin
antibody (Abcam, Cambridge, MA) as loading control. After incubation with
HRP-conjugated secondary antibodies, membranes were developed with SuperSignal
West Dura Chemiluminescence Substrate (Thermo Fisher Scientific Inc., Rockford,
IL) using ChemiDoc MP Imaging System (Bio-Rad, Hercules, CA). The bands were
semi-quantified by densitometry.

Tang X., Wang J.J., Wang J., Abboud H.E., Chen Y, & Zhang S.X. (2020). Endothelium-specific Deletion of Nox4 Delays Retinal Vascular Development and Mitigates Pathological Angiogenesis. Angiogenesis, 24(2), 363-377.

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