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2 protocols using c19 sc 711

1

Antibody Immunoblotting for Insulin Signaling

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The rabbit polyclonal anti-insulin receptor (IR) β-subunit (C19; sc-711), the mouse monoclonal anti-phosphotyrosine (PY99; sc-7020), the goat polyclonal anti-rabbit IgG conjugated with HRP (sc-2004), the goat anti-mouse IgG-HRP (sc-2005) and rabbit anti-goat IgG-HRP (sc-2768) antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). The rabbit polyclonal antibody anti-phospho-Akt-S473 (4060), the rabbit polyclonal antibody anti-phospho-Akt-T308 (9275), the anti-Akt (pan) rabbit monoclonal antibody (C67E7) that detects endogenous levels of total AKT1, AKT2, and AKT3 protein, the rabbit monoclonal, the rabbit monoclonal antibody anti-phospho-ERK1/2, that detects ERK-1 and −2 (p44 and p42 MAPK respectively) when phosphorylated at T202 and Y204 (4370) and the rabbit polyclonal antibody anti-ERK1/2 (9102) were from Cell Signalling (Danvers, MA, USA). The goat polyclonal anti-adiponectin (AF1119) antibody was from R&D systems (Minneapolis, MN, USA). The rabbit polyclonal antibody anti-UCP-1 (PA1–24894 was from Thermo Fisher (Rockford, IL, USA). Protein loading in the gels was evaluated with a polyclonal anti-actin antibody produced in rabbit (Sigma-Aldrich, USA, Catalog Number A2066).
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2

Antibodies for Molecular Signaling Analysis

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The rabbit polyclonal antiinsulin receptor (IR) β‐subunit (C19; sc‐711), the mouse monoclonal antiphosphotyrosine (PY99; sc‐7020), the goat polyclonal anti‐rabbit IgG conjugated with HRP (sc‐2004), the goat anti‐mouse IgG‐HRP (sc‐2005), and rabbit anti‐goat IgG‐HRP (sc‐2768) antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). The rabbit polyclonal antibody antiphospho‐Akt‐S473 (4060), the rabbit polyclonal antibody antiphospho‐Akt‐T308 (9275), the anti‐Akt (pan) rabbit monoclonal antibody (C67E7) that detects endogenous levels of total AKT1, AKT2, and AKT3 protein, the rabbit monoclonal, the rabbit monoclonal antibody antiphospho‐ERK1/2, that detects ERK‐1 and ‐2 (p44 and p42 MAPK, respectively) when phosphorylated at T202 and Y204 (4370) and the rabbit polyclonal antibody anti‐ERK1/2 (9102) were from Cell Signalling (Danvers, MA). The goat polyclonal anti‐mouse TNF‐α (MAB4101) and antiadiponectin (AF1119) antibodies were from R&D systems (Minneapolis, MN). The rabbit polyclonal antibody antinitrotyrosine (AB5411) was from Millipore Sigma (St. Louis, MO). The rabbit polyclonal antibody anti‐UCP‐1 (PA1‐24894 was from Thermo Fisher (Rockford, IL). Protein loading in the gels was evaluated with a mouse monoclonal anti‐GADPDH (ab9485) from Abcam (San Francisco, CA).
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